The role of KIF14 in patient-derived primary cultures of high-grade serous ovarian cancer cells
Conclusions:
Although primary high-grade serous OvCa cells may depend on KIF14 for in vitro proliferation we were unable to demonstrate a role for KIF14 on tumorigenicity or develop an in vivo model for assessment. We have, however developed an effective in vitro method to evaluate the effect of target gene manipulation on the proliferative capacity of primary OvCa cultures. (Source: Journal of Ovarian Research)
Source: Journal of Ovarian Research - December 21, 2014 Category: Cancer & Oncology Authors: Brigitte ThériaultPaulina CybulskaPatricia ShawBrenda GallieMarcus Bernardini Source Type: research
Sequential combination therapy with flavopiridol and autocatalytic caspase-3 driven by amplified hTERT promoter synergistically suppresses human ovarian carcinoma growth in vitro and in mice
In this study, we applied it with flavopiridol to investigate their antitumor effect on ovarian cancer in vitro and in vivo.
Methods:
Cell viabilities were determined using Cell Counting Kit 8 and flow cytometry. RT-PCR and immunoblotting assays were used to detect cellular apoptotic activities. Tumor growth and survival of mice bearing tumors were studied.
Results:
Flavopiridol or AdHTVP2G5-rev-casp3 at low dosage alone was mildly cytotoxic in vitro with a viability rate of 86.5???4.7% for 300 nM flavopiridol and 88.9???5.4% for AdHTVP2G5-rev-casp3 (MOI 20). By contrast, significant synergism of their sequential combinati...
Source: Journal of Ovarian Research - December 21, 2014 Category: Cancer & Oncology Authors: Yue SongXing XinXingyue ZhaiZhijun XiaKeng Shen Source Type: research
The role of KIF14 in patient-derived primary cultures of high-grade serous ovarian cancer cells
Conclusions:
Although primary high-grade serous OvCa cells may depend on KIF14 for in vitro proliferation we were unable to demonstrate a role for KIF14 on tumorigenicity or develop an in vivo model for assessment. We have, however developed an effective in vitro method to evaluate the effect of target gene manipulation on the proliferative capacity of primary OvCa cultures. (Source: Journal of Ovarian Research)
Source: Journal of Ovarian Research - December 21, 2014 Category: Cancer & Oncology Authors: Brigitte ThériaultPaulina CybulskaPatricia ShawBrenda GallieMarcus Bernardini Source Type: research
Evaluating influence of the genotypes in the follicle-stimulating hormone receptor (FSHR) Ser680Asn (rs6166) polymorphism on poor and hyper-responders to ovarian stimulation: a meta-analysis
Conclusions:
In summary, this is a meta-analytical confirmation of the FSHR SS genotype role in COH response. Hyper-responder analysis strengths lie on the non-heterogeneity and robustness of its results. Non-robustness and heterogeneity of the poor-responder results compose its limitations. Thus, poor response findings probably require caution as to the interpretation as a susceptibility marker for ovarian response. (Source: Journal of Ovarian Research)
Source: Journal of Ovarian Research - December 20, 2014 Category: Cancer & Oncology Authors: Noel PabalanCamila TrevisanCarla PelusoHamdi JarjanaziDenise ChristofoliniCaio BarbosaBianca Bianco Source Type: research
ESR1 and ESR2 gene polymorphisms are associated with human reproduction outcomes in Brazilian women
Background:
Important candidate genes involved in the ovarian response to exogenous FSH are the estrogen receptor genes (ESRs), since the effects of estrogens on follicle growth, maturation and oocyte release. It is known that some markers of ovarian stimulation can help to personalize the treatment, adjusting the dose of exogenous rFSH, thus preventing excessive wear of the patient. Inspired on this information we aimed to analyze four different polymorphisms in the estrogen receptor genes ESR1: rs2234693/T-397C (PvuII) and rs9340799/A-351G (Xbal) and ESR2: rs4986938/G1082A (RsaI) and rs1256049/A?+?1730G (AluI), and their...
Source: Journal of Ovarian Research - December 20, 2014 Category: Cancer & Oncology Authors: Clarissa de MattosCamila TrevisanCarla PelusoFernando AdamiEmerson CordtsDenise ChristofoliniCaio BarbosaBianca Bianco Source Type: research
Evaluating influence of the genotypes in the follicle-stimulating hormone receptor (FSHR) Ser680Asn (rs6166) polymorphism on poor and hyper-responders to ovarian stimulation: a meta-analysis
Conclusions:
In summary, this is a meta-analytical confirmation of the FSHR SS genotype role in COH response. Hyper-responder analysis strengths lie on the non-heterogeneity and robustness of its results. Non-robustness and heterogeneity of the poor-responder results compose its limitations. Thus, poor response findings probably require caution as to the interpretation as a susceptibility marker for ovarian response. (Source: Journal of Ovarian Research)
Source: Journal of Ovarian Research - December 20, 2014 Category: Cancer & Oncology Authors: Noel PabalanCamila TrevisanCarla PelusoHamdi JarjanaziDenise ChristofoliniCaio BarbosaBianca Bianco Source Type: research
Anti Müllerian Hormone (AMH) level and expression in mural and cumulus cells in relation to age
This study investigates AMH level in the follicular fluid (FF) and mRNA expression pattern in cumulus and mural granulosa cells of human ovarian follicles in relation to age.
Methods:
We conducted a prospective study. Sixty-eight women undergoing In vitro fertilization (IVF) treatment were enrolled in the study. We obtained FF, mural and cumulus granulosa cells from large preovulatory follicles (17-20?mm) of 21?35 years old women (n?=?40) and 40?45 years old women (n?=?28) during oocyte pickup.
Results:
Higher level of AMH mRNA expression in cumulus cells was observed in the older age group compared to the younger (P (Sour...
Source: Journal of Ovarian Research - December 11, 2014 Category: Cancer & Oncology Authors: Alon KedemYuval YungGil YerushalmiJigal HaasEttie MamanMirit HanochiRina HemiRaoul OrvietoJehoshua DorAriel Hourvitz Source Type: research
Succinate dehydrogenase subunit B inhibits the AMPK-HIF-1¿ pathway in human ovarian cancer in vitro
Conclusions:
Our results firstly revealed that SDHB played a key role in cell proliferation, invasion, migration, and apoptosis of human ovarian carcinoma via AMPK-HIF-1α pathway. SDHB-overexpression might be a new approach to inhibit tumor progression in human ovarian carcinoma. (Source: Journal of Ovarian Research)
Source: Journal of Ovarian Research - December 10, 2014 Category: Cancer & Oncology Authors: Lilan ChenTing LiuShu ZhangJinhua ZhouYunfei WangWen Di Source Type: research
Protein kinase C isoforms ¿, ¿ and ¿ are differentially expressed in mouse ovaries at different stages of postnatal development
Conclusions:
This study demonstrates that PKCα, PKCδ and PKCε isoforms are differentially expressed in particular cellular components of pre-pubertal, pubertal and adult mouse ovarian follicles. Therefore, we suggest that each PKC isoform has unique functions that are controlled by gonadotropin dependent mechanisms during follicular growth, oocyte maturation, ovulation and luteinization. (Source: Journal of Ovarian Research)
Source: Journal of Ovarian Research - December 10, 2014 Category: Cancer & Oncology Authors: Filiz TepekoyIsmail UstunelGokhan Akkoyunlu Source Type: research
Succinate dehydrogenase subunit B inhibits the AMPK-HIF-1¿ pathway in human ovarian cancer in vitro
Conclusions:
Our results firstly revealed that SDHB played a key role in cell proliferation, invasion, migration, and apoptosis of human ovarian carcinoma via AMPK-HIF-1? pathway. SDHB-overexpression might be a new approach to inhibit tumor progression in human ovarian carcinoma. (Source: Journal of Ovarian Research)
Source: Journal of Ovarian Research - December 10, 2014 Category: Cancer & Oncology Authors: Lilan ChenTing LiuShu ZhangJinhua ZhouYunfei WangWen Di Source Type: research
Does bariatric surgery improve ovarian stimulation characteristics, oocyte yield, or embryo quality?
Conclusions:
To the best of our knowledge, this preliminary case series is the first comparison of IVF cycle characteristics prior to and following bariatric surgery. The operation seems to reduce treatment costs without affecting oocyte or embryo quality. Further large studies are required to establish the surgery?s effect on IVF outcome among infertile women. (Source: Journal of Ovarian Research)
Source: Journal of Ovarian Research - December 10, 2014 Category: Cancer & Oncology Authors: Abraham TsurRaoul OrvietoJigal HaasAlon KedemRonit Machtinger Source Type: research
Protein kinase C isoforms ¿, ¿ and ¿ are differentially expressed in mouse ovaries at different stages of postnatal development
Conclusions:
This study demonstrates that PKC?, PKC? and PKC? isoforms are differentially expressed in particular cellular components of pre-pubertal, pubertal and adult mouse ovarian follicles. Therefore, we suggest that each PKC isoform has unique functions that are controlled by gonadotropin dependent mechanisms during follicular growth, oocyte maturation, ovulation and luteinization. (Source: Journal of Ovarian Research)
Source: Journal of Ovarian Research - December 10, 2014 Category: Cancer & Oncology Authors: Filiz TepekoyIsmail UstunelGokhan Akkoyunlu Source Type: research
Wilms¿ tumor suppressor gene ( WT1 ) suppresses apoptosis by transcriptionally downregulating BAX expression in immature rat granulosa cells
Conclusion:
These findings suggest that activation of WT1 is necessary for maintenance of GC survival during early stage of follicles and WT1 can play a role in protecting apoptosis through the regulation of upstream activator (Bax), as well as through regulation of downstream effecter (caspases 3 and 7). (Source: Journal of Ovarian Research)
Source: Journal of Ovarian Research - December 10, 2014 Category: Cancer & Oncology Authors: Minji ParkYuri ChoiHyeonhae ChoiJaesook Roh Source Type: research
Kallikrein family proteases KLK6 and KLK7 are potential early detection and diagnostic biomarkers for serous and papillary serous ovarian cancer subtypes
Conclusion:
KLK6 and KLK7 mRNA and protein overexpression is directly associated with early-stage ovarian tumors and can be measured in patient tissue and serum samples. Assays based on KLK6 and KLK7 expression may provide specific and sensitive information for early detection of ovarian cancer. (Source: Journal of Ovarian Research)
Source: Journal of Ovarian Research - December 5, 2014 Category: Cancer & Oncology Authors: Ayala TamirUshma JagSreeja SarojiniCraig SchindewolfTakemi TanakaRajendra GharbaranHiren PatelAnil SoodWei HuRuzeen PatwaPatrick BlakePolina ChirinaJin Oh JeongHeejin LimAndre GoyAndrew PecoraK Suh Source Type: research
Kallikrein family proteases KLK6 and KLK7 are potential early detection and diagnostic biomarkers for serous and papillary serous ovarian cancer subtypes
Conclusion:
KLK6 and KLK7 mRNA and protein overexpression is directly associated with early-stage ovarian tumors and can be measured in patient tissue and serum samples. Assays based on KLK6 and KLK7 expression may provide specific and sensitive information for early detection of ovarian cancer. (Source: Journal of Ovarian Research)
Source: Journal of Ovarian Research - December 5, 2014 Category: Cancer & Oncology Authors: Ayala TamirUshma JagSreeja SarojiniCraig SchindewolfTakemi TanakaRajendra GharbaranHiren PatelAnil SoodWei HuRuzeen PatwaPatrick BlakePolina ChirinaJin Oh JeongHeejin LimAndre GoyAndrew PecoraK Suh Source Type: research
