Antisense oligonucleotide suppression of human IGF-1R inhibits the growth and survival of in vitro cultured epithelial ovarian cancer cells
Conclusions:
IGF-IR enhances the proliferation and tumorigenicity of human ovarian cancer cells and inhibition of IGF-IR by AS oligonucleotide treatment potentiates the activity of cisplatin in vitro. Therefore, IGF-1R is a potential molecular target in ovarian cancer. (Source: Journal of Ovarian Research)
Source: Journal of Ovarian Research - October 8, 2013 Category: Cancer & Oncology Authors: Jie TangJunjun LiGuqing ZengYanxiang TangWenfang TianJie HeJ YorkXuefeng Xia Source Type: research
Pioglitazone counteracts the tumor necrosis factor-¿ inhibition of follicle-stimulating hormone-induced follicular development and estradiol production in an in vitro mouse preantral follicle culture system
Background:
Polycystic ovary syndrome (PCOS) is a common endocrine disorder in women of reproductive age and is characterized by chronic anovulation. Insulin resistance may be a key component of the pathogenesis of this disorder. Pioglitazone is a thiazolidinedione derivative that acts by improving insulin resistance via the peroxisome proliferator-activated receptor-γ (PPAR-γ) pathway. Reportedly, pioglitazone improves the anovulation status in patients with PCOS. In the present study, we examined whether pioglitazone directly affects ovarian follicular development and steroidogenesis using in vitro mouse preantral foll...
Source: Journal of Ovarian Research - September 30, 2013 Category: Cancer & Oncology Authors: Shuichiro HaraToshifumi TakahashiMitsuyoshi AmitaKoki MatsuoHideki IgarashiHirohisa Kurachi Source Type: research
FSH in vitro versus LH in vivo: similar genomic effects on the cumulus
The use of gonadotropins to trigger oocyte maturation both in vivo and in vitro has provided precious and powerful knowledge that has significantly increased our understanding of the ovarian function. Moreover, the efficacy of most assisted reproductive technologies (ART) used in both humans and livestock species relies on gonadotropin input, mainly FSH and LH. Despite the significant progress achieved and the huge impact of gonadotropins, the exact molecular pathways of the two pituitary hormones, FSH and LH, still remain poorly understood. Moreover, these pathways may not be the same when moving from the in vivo to the i...
Source: Journal of Ovarian Research - September 25, 2013 Category: Cancer & Oncology Authors: Mourad AssidiFrançois RichardMarc-André Sirard Source Type: research
The effect of calorie restriction on the presence of apoptotic ovarian cells in normal wild type mice and low-plasma-IGF-1 Laron dwarf mice
Conclusions:
Morphological and histochemical analysis revealed a younger biological age of the ovaries in 2-year-old WT littermates and GHR-KO female mice on CR compared with animals fed AL. (Source: Journal of Ovarian Research)
Source: Journal of Ovarian Research - September 24, 2013 Category: Cancer & Oncology Authors: Sylwia S¿uczanowska-G¿¿bowskaMaria Laszczy¿skaKatarzyna PiotrowskaWojciech G¿¿bowskiBogdan RumianowskiMichal MasternakOge ArumMagda KuciaJohn KopchickAndrzej BartkeMariusz Ratajczak Source Type: research
Ovarian stem cells: absence of evidence is not evidence of absence
Conclusions:
Cysts represent self-renewal and clonal expansion of stem cells with incomplete cytokinesis and are a hallmark feature of stem cells. We suggest the use of PMSG stimulated mouse ovaries and use of more primitive markers like OCT-4 or STELLA rather than MVH for lineage tracing studies to conclusively show presence of stem cells by lineage-tracing studies. (Source: Journal of Ovarian Research)
Source: Journal of Ovarian Research - September 17, 2013 Category: Cancer & Oncology Authors: Deepa BhartiyaKalpana SriramanSeema ParteHiren Patel Source Type: research
Enhanced levels of double-strand DNA break repair proteins protect ovarian cancer cells against genotoxic stress-induced apoptosis
Conclusions:
Present study elucidates a functional relevance of NPM1, RAD50 and XRCC5 DSB-repair proteins towards ensuring survival and evasion of apoptosis during ovarian transformation, emphasizing their contribution and association with disease progression in high-grade SeOvCa. (Source: Journal of Ovarian Research)
Source: Journal of Ovarian Research - September 17, 2013 Category: Cancer & Oncology Authors: Rajkumar KalraSharmila Bapat Source Type: research
Pronuclear morphology evaluation for fresh in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) cycles: a systematic review
In conclusion, our findings demonstrate the lack of conclusive data on the clinical efficacy of the zygote morphology evaluation in fresh IVF/ICSI cycles, even if biological results showing a good relationship with embryo viability suggest a role in cycles in which the transfer/freezing is performed at day 1. (Source: Journal of Ovarian Research)
Source: Journal of Ovarian Research - September 12, 2013 Category: Cancer & Oncology Authors: Alessia NicoliStefano PalombaFrancesco CapodannoMaria FiniAngela FalboGiovanni La Sala Source Type: research
Tracking NF-¿B activity in tumor cells during ovarian cancer progression in a syngeneic mouse model
Conclusions:
Our ID8-NGL reporter syngeneic model is suitable for investigating changes in tumor NF-kappaB activity during ovarian cancer progression, how NF-kappaB activity influences immune cells in the tumor microenvironment, and effects of NF-kappaB-targeted treatments in future studies. (Source: Journal of Ovarian Research)
Source: Journal of Ovarian Research - September 10, 2013 Category: Cancer & Oncology Authors: Andrew WilsonWhitney BarhamJeanette SaskowskiOleg TikhomirovLianyi ChenHye-Jeong LeeFiona YullDineo Khabele Source Type: research
Tracking NF-kappaB activity in tumor cells during ovarian cancer progression in a syngeneic mouse model
Conclusions:
Our ID8-NGL reporter syngeneic model is suitable for investigating changes in tumor NF-kappaB activity during ovarian cancer progression, how NF-kappaB activity influences immune cells in the tumor microenvironment, and effects of NF-kappaB-targeted treatments in future studies. (Source: Journal of Ovarian Research)
Source: Journal of Ovarian Research - September 10, 2013 Category: Cancer & Oncology Authors: Andrew WilsonWhitney BarhamJeanette SaskowskiOleg TikhomirovLianyi ChenHye-Jeong LeeFiona YullDineo Khabele Source Type: research
Metformin decreases the incidence of ovarian hyperstimulation syndrome: an experimental study
Background:
In assisted reproduction cycles, gonadotropins are administered to obtain a greater number of oocytes. A majority of patients do not have an adverse response; however, approximately 3-6% develop ovarian hyperstimulation syndrome (OHSS). Metformin reduces the risk of OHSS but little is known about the possible effects and mechanisms of action involved.ObjectiveTo evaluate whether metformin attenuates some of the ovarian adverse effects caused by OHSS and to study the mechanisms involved.Material and methodsA rat OHSS model was used to investigate the effects of metformin administration. Ovarian histology and fol...
Source: Journal of Ovarian Research - September 8, 2013 Category: Cancer & Oncology Authors: Evelin EliaRamiro QuintanaCarlos CarrereVictoria BazzanoGastón Rey-ValzacchiDante PazCarolina Pustovrh Source Type: research
Splice variants of zinc finger protein 695 mRNA associated to ovarian cancer
Conclusions:
Alternative ZNF695 mRNA splicing could be a marker of ovarian cancer with possible implications on its pathogenesis. (Source: Journal of Ovarian Research)
Source: Journal of Ovarian Research - September 5, 2013 Category: Cancer & Oncology Authors: Sergio Juárez-MéndezAlejandro ZentellaVanessa Villegas-RuízOscar Pérez-GonzálezMauricio SalcedoRicardo López-RomeroEdgar Román-BasaureMinerva Lazos-OchoaVíctor Montes de Oca-FuentesGuelaguetza Vázquez-OrtizJosé Moreno Source Type: research
Normalizing to GADPH jeopardises correct quantification of gene expression in ovarian tumours ¿ IPO8 and RPL4 are reliable reference genes
Conclusions:
We found IPO8 and RPL4 to be suitable reference genes for normalization of target gene expression in benign, borderline, and malignant ovarian tumours. Moreover, IPO8 can be recommended as a single reference gene. Neither GADPH nor HPRT1 should be used as reference genes in studies on ovarian tumour tissue. (Source: Journal of Ovarian Research)
Source: Journal of Ovarian Research - August 30, 2013 Category: Cancer & Oncology Authors: Zuzana KolkovaArsen ArakelyanBertil CasslénStefan HanssonEva Kriegova Source Type: research
Normalizing to GADPH jeopardises correct quantification of gene expression in ovarian tumours -- IPO8 and RPL4 are reliable reference genes
Conclusions:
We found IPO8 and RPL4 to be suitable reference genes for normalization of target gene expression in benign, borderline, and malignant ovarian tumours. Moreover, IPO8 can be recommended as a single reference gene. Neither GADPH nor HPRT1 should be used as reference genes in studies on ovarian tumour tissue. (Source: Journal of Ovarian Research)
Source: Journal of Ovarian Research - August 30, 2013 Category: Cancer & Oncology Authors: Zuzana KolkovaArsen ArakelyanBertil CasslénStefan HanssonEva Kriegova Source Type: research
Simultaneous early ovarian and endometrial cancer treated conservatively with spontaneous pregnancy
Conclusion:
This case demonstrates some interesting and unique features of synchronous ovarian and endometrial cancers since it resulted in a spontaneous pregnancy and normal delivery. (Source: Journal of Ovarian Research)
Source: Journal of Ovarian Research - August 21, 2013 Category: Cancer & Oncology Authors: David AtallahJoelle SafiNadine el KassisRoman RouzierGeorges Chahine Source Type: research
Value of 3.0 T diffusion-weighted imaging in discriminating thecoma and fibrothecoma from other adnexal solid masses
Conclusions:
There is no significant difference in ADC between thecoma and other adnexal solid masses, but the ADCs of thecomas are lower than those of leiomyomas. (Source: Journal of Ovarian Research)
Source: Journal of Ovarian Research - August 21, 2013 Category: Cancer & Oncology Authors: He ZhangGuo-Fu ZhangTian-Ping WangHao Zhang Source Type: research
