Normalizing to GADPH jeopardises correct quantification of gene expression in ovarian tumours ¿ IPO8 and RPL4 are reliable reference genes

Conclusions: We found IPO8 and RPL4 to be suitable reference genes for normalization of target gene expression in benign, borderline, and malignant ovarian tumours. Moreover, IPO8 can be recommended as a single reference gene. Neither GADPH nor HPRT1 should be used as reference genes in studies on ovarian tumour tissue.
Source: Journal of Ovarian Research - Category: Cancer & Oncology Authors: Source Type: research