Systemic oxidative stress in victims of Bothrops snakebites
Publication date: Available online 1 December 2014 Source:Journal of Applied Biomedicine Author(s): Juliana de Ornellas Strapazzon , Eduardo Benedetti Parisotto , Ana Maria Moratelli , Thais Regina Garlet , Juliana Bastos , Ivan Ricardo Zimermann , Marlene Zanin , Rodrigo Fagundez , Manuel Rosa de Oliveira Lino , Tânia Silvia Fröde , Danilo Wilhelm-Filho The genus Bothrops is responsible for approximately 90% of snakebites in Brazil. In the present study biomarkers of oxidative stress (OS) were evaluated in the blood of victims of snakebites from Bothrops jararaca and Bothrops jararacussu. Patient monitori...
Source: Journal of Applied Biomedicine - December 2, 2014 Category: Biotechnology Source Type: research
Multinuclear magnetic resonance evaluation of tumor tissue
Publication date: Available online 8 November 2014 Source:Journal of Applied Biomedicine Author(s): Dorota Bartusik , David Aebisher Current biomedical research requires quantitative and qualitative studies of tissue metabolites. In this review, we discuss recent advances in multinuclear magnetic resonance (MR) technology that enhance biomedical research. The advances presented herein clearly show that MR is a diagnostic tool for the study of tumor tissue metabolism associated with changes in small molecule concentrations. Biomedical MR offers a non-invasive view of cancer cell metabolism by detection of resonance nu...
Source: Journal of Applied Biomedicine - November 9, 2014 Category: Biotechnology Source Type: research
Effects of therapeutic ultrasound on the nuclear envelope and nuclear pore complexes
Publication date: 2013 Source:Journal of Applied Biomedicine, Volume 11, Issue 4 Author(s): Naděžda Vaškovicová , Zdena Druckmüllerová , Roman Janisch , Jiřina Škorpíková , Vojtěch Mornstein The effects of acoustic waves on membrane structures, and any resulting consequences of this treatment on membrane subunit structures, remain poorly understood, as are the principals of related clinical effects. With a focus on morphological changes in the nuclear envelope, the current study presents detailed observations of membrane structures exposed to therapeutic ultrasound. Ultrasound treatment most commonly re...
Source: Journal of Applied Biomedicine - November 3, 2014 Category: Biotechnology Source Type: research
Nitrite contamination in hypotensive preparations of dinitrosyl iron complexes with glutathione
Publication date: 2013 Source:Journal of Applied Biomedicine, Volume 11, Issue 4 Author(s): Rostislav Romanovich Borodulin , Vyacheslav Olegovich Shvydkiy , Lyudmila Nikolaevna Kubrina , Vasak Dzhanibekovich Mikoyan , Natalya Ilinishna Zaitseva , Tatyana Evgenjevna Sporyagina , Sergey Alexandrovich Kryuchkov , Anatoly Fjodorovich Vanin The content of nitrite admixture in preparations of dinitrosyl iron complexes (DNIC) with glutathione synthesized by treatment of aqueous solutions of Fe2+ + glutathione with gaseous NO (complex 1) or by mixing solutions of S-nitrosoglutathione (GS-NO) with solutions of Fe2+ + gl...
Source: Journal of Applied Biomedicine - November 3, 2014 Category: Biotechnology Source Type: research
Relationship between serum bilirubin and uric acid to oxidative stress markers in Italian and Czech populations
Publication date: 2013 Source:Journal of Applied Biomedicine, Volume 11, Issue 4 Author(s): Libor Vítek , Ladislav Novotný , Aleš Žák , Barbora Staňková , Tomáš Zima , Angela Polito , Greco Cesare , Chiara Zerbinati , Luigi Iuliano Recently, a South-to-North oxidative stress marker gradient has been reported; consistent with known differences in the incidence of coronary heart disease between southern and northern European countries. The aim of the present study was to compare the plasma concentrations of 7-oxocholesterol (7OxCH) and 7β-hydroxycholesterol (7BCH) with systemic antioxidants in healthy ...
Source: Journal of Applied Biomedicine - November 3, 2014 Category: Biotechnology Source Type: research
Proenzyme therapy of sarcoma S-180 and melanoma B16-F10
Publication date: January 2014 Source:Journal of Applied Biomedicine, Volume 12, Issue 1 Author(s): Pavlína Kaiserová , Lucie Kalferstová , Hana Maršíková , Irena Bartoňová , Peter Koník , Jan Kopecký , Jan Ženka The aim of this study was to evaluate the effectiveness of individual (inactive) proenzymes and mixtures thereof in cancer treatment and to compare this treatment with more frequently used therapy based on active proteases. Experiments focused on explanation of possible mechanisms of proenzyme action against tumours are included. Proenzyme therapy of sarcoma S-180 significantly reduced tumour...
Source: Journal of Applied Biomedicine - November 3, 2014 Category: Biotechnology Source Type: research
Circadian haematotoxicity of the antiepileptic valproic acid in mice
The objective of this study is to experimentally verify if VPA-induced haematological toxicity in mice varies according to drug administration time in the 24h scale. Different groups of mice received 620mg/kg of VPA by i.p. route at four different circadian stages. The results obtained showed that VPA treatment induced a significant decrease in the haematological parameter rates (cytopenia) depending on the circadian time; otherwise the Cosinor analysis showed that each haematological variable followed a significant blood type circadian physiological rhythm in controls and in treated mice. The highest significant haematolo...
Source: Journal of Applied Biomedicine - November 3, 2014 Category: Biotechnology Source Type: research
Entry of oxime K027 into the different parts of rat brain: Comparison with obidoxime and oxime HI-6
This study aims to verify oxime penetration through the blood–brain barrier, and into different brain regions. It has been confirmed that oximes can penetrate the BBB after therapeutic dose administration (0.73%, 0.69%, and 0.52% corresponding to obidoxime, HI-6, and new oxime K027). Although the whole brain level of K027 seems to be lower compared with other oximes we have to keep in mind that these numbers represent only a percentage of the concentration found in plasma. So, for more adequate comparison it is essential to have a look at the actual concentration of oximes in the wet brain tissue: 106.6±19.87ng/g (K027)...
Source: Journal of Applied Biomedicine - November 3, 2014 Category: Biotechnology Source Type: research
Fate of two types of cerebellar graft in wild type and cerebellar mutant mice
Publication date: January 2014 Source:Journal of Applied Biomedicine, Volume 12, Issue 1 Author(s): Miroslava Čedíková , Zbyněk Houdek , Václav Babuška , Vlastimil Kulda , František Vožeh , Nicolas Zech , Lenka Černá , Kristýna Krakorová , Milena Králíčková , Jan Cendelín The possibility of regeneration in a damaged central nervous system (CNS) is limited and therapy of CNS diseases accompanied with neuronal loss is problematic. Lurcher (Lc) mutant mice represent a model for olivocerebellar degeneration. Heterozygotes suffer from a complete loss of cerebellar Purkinje cells and a secondary re...
Source: Journal of Applied Biomedicine - November 3, 2014 Category: Biotechnology Source Type: research
Advances in biosensors: Principle, architecture and applications
Publication date: January 2014 Source:Journal of Applied Biomedicine, Volume 12, Issue 1 Author(s): Veeradasan Perumal , Uda Hashim The ability to detect pathogenic and physiologically relevant molecules in the body with high sensitivity and specificity offers a powerful opportunity in the early diagnosis and treatment of diseases. Early detection and diagnosis can be used to greatly reduce the cost of patient care associated with the advanced stages of many diseases. However, despite their widespread clinical use, these techniques have a number of potential limitations. For example, a number of diagnostic devices ha...
Source: Journal of Applied Biomedicine - November 3, 2014 Category: Biotechnology Source Type: research
HPLC analysis and antimicrobial, antimycobacterial and antiviral activities of Tabernaemontana catharinensis A. DC
Publication date: Available online 4 February 2014 Source:Journal of Applied Biomedicine Author(s): Aline Augusti Boligon , Mariana Piana , Thaís Felli Kubiça , Débora Nunes Mario , Tanise Vendruscolo Dalmolin , Pauline Cordenonsi Bonez , Rudi Weiblen , Luciane Lovato , Sydney Hartz Alves , Marli M.A. Campos , Margareth Linde Athayde Antimicrobial, antimycobacterial and antiviral activities of crude extracts, fractions and subfractions of Tabernaemontana catharinensis were evaluated. Best antimicrobial results occurred with the dichloromethane (DCM) and butanolic (NB) fractions against Staphylococcus aure...
Source: Journal of Applied Biomedicine - November 3, 2014 Category: Biotechnology Source Type: research
A comparison of cholinesterase inhibitors in the treatment of quinuclidinyl benzilate-induced behavioural deficit in rats performing the multiple T-maze
Publication date: Available online 4 February 2014 Source:Journal of Applied Biomedicine Author(s): Jan Misik , Jiri Kassa Cholinesterase inhibitors are beneficial in the treatment of Alzheimer's disease via indirect increase of cholinergic neuro-transmission. The aim of the present study was to evaluate the potency of inhibitors tacrine, rivastigmine and donepezil to reverse cholinergic depletion induced by 3-quinuclidinyl benzilate (QNB, 2mgkg−1) in Wistar rats performing the multiple T-maze test. The effect of QNB on retention was compared to the effect of standard amnesic drug, scopolamine, at the dose of 0.3mg...
Source: Journal of Applied Biomedicine - November 3, 2014 Category: Biotechnology Source Type: research
Avidity of anticardiolipin antibodies—A factor that could be important for their detection by ELISA methods
Publication date: Available online 4 February 2014 Source:Journal of Applied Biomedicine Author(s): Lenka Fialová , Ivan Malbohan , Karin Malíčková Avidity is an important feature of antibodies associated with their pathogenic effects. Anticardiolipin antibodies (aCLs) are the most commonly examined antiphospholipid antibodies, however, their avidity has been investigated only marginally. The aim of the study was to compare the avidity of the antibodies specifically bound to the cardiolipin-coated microtitrate wells with those bound to the wells bearing no cardiolipin in various conditions. We analysed 22 serum ...
Source: Journal of Applied Biomedicine - November 3, 2014 Category: Biotechnology Source Type: research
Parallelism of circadian rhythmicity of salivary and serum cortisol concentration in normal dogs
Publication date: Available online 6 February 2014 Source:Journal of Applied Biomedicine Author(s): Claudia Giannetto , Francesco Fazio , Anna Assenza , Daniela Alberghina , Michele Panzera , Giuseppe Piccione To evaluate parallel circadian rhythms in salivary and serum cortisol concentrations during 48-h period, sampling was performed in six clinically healthy dogs of various breeds housed under natural photoperiod in spring (sunrise 05:20, sunset 20:20). Saliva and blood samples were taken every 3h for a 48-h period to determine the daily changes in salivary and serum cortisol concentrations. The relationship b...
Source: Journal of Applied Biomedicine - November 3, 2014 Category: Biotechnology Source Type: research
Low molecular weight precursor applicable for Alzheimer disease drugs synthesis (AChE and BChE inhibition, BACE inhibition, antioxidant properties and in silico modulation)
This article focuses on possibility to find the most suitable chemical precursors to be used in the next development of drugs for AD. (Source: Journal of Applied Biomedicine)
Source: Journal of Applied Biomedicine - November 3, 2014 Category: Biotechnology Source Type: research
