Red American ginseng enhances the effect of fluorouracil on human colon cancer cells via both paraptosis and apoptosis pathways
Conclusion RAG may have clinical utility to decrease the dosage of 5-FU in colorectal cancer therapeutics. Graphical abstract (Source: Journal of Applied Biomedicine)
Source: Journal of Applied Biomedicine - June 1, 2018 Category: Biotechnology Source Type: research
Adipocytokines and new onset diabetes mellitus after transplantation
Publication date: Available online 31 May 2018 Source:Journal of Applied Biomedicine Author(s): Dominika Goldmannová, Jaromíra Spurná, Ondřej Krystyník, Jan Schovánek, Lubica Cibičková, David Karásek, Josef Zadražil Background and aim Diabetes mellitus is a very common metabolic disease with a rising incidence. It is one of the most serious comorbidities in renal transplant recipients. New-onset diabetes after renal transplantation (NODAT) is associated with poor graft function, higher rates of cardiovascular complications and a poor prognosis. Adipocytokines, synthetized by adipose tissue influence meta...
Source: Journal of Applied Biomedicine - June 1, 2018 Category: Biotechnology Source Type: research
Effect of Pseuduvaria macrophylla in attenuating hyperglycemia mediated oxidative stress and inflammatory response in STZ-nicotinamide induced diabetic rats by upregulating insulin secretion and glucose transporter-1, 2 and 4 proteins expression
This study aimed to ascertain antidiabetic potential of methanolic extract of Pseuduvaria macrophylla bark (PM) using streptozotocin-nicotinamide induced diabetic rat models. Various phytochemical and biochemical properties of the plant have been evaluated. The results showed that the extract has potentially normalized the elevated blood glucose levels by upregulating the insulin and C-peptide levels and alleviated oxidative stress by improving glutathione (GSH) and reducing lipid peroxidation (LPO) in the diabetic rats. In addition, PM has drastically downregulated the levels of pro-inflammatory cytokines and transforming...
Source: Journal of Applied Biomedicine - May 27, 2018 Category: Biotechnology Source Type: research
Effect of different loads of treadmill exercise on Th1/Th2 cytokine balance in rat splenocytes
Publication date: Available online 24 May 2018 Source:Journal of Applied Biomedicine Author(s): Zahra Gholamnezhad, Mohammad Hossein Boskabady, Mahmoud Hosseini The effect of moderate and overtraining exercise on Th1/Th2 balance was evaluated in rat splenocytes. Male Wistar rats were divided into sedentary control (C), moderately trained (MT; V = 20 m/min, 30 min/day, 8 weeks), overtrained (OT; V = 25 m/min, 60 min/day, 11 weeks) and recovered after overtraining (OR) (OT plus 2 weeks recovery) groups. At the end of study, cell viability, proliferation, interleukin 4 (IL-4) and interferon-γ (IFN-γ) se...
Source: Journal of Applied Biomedicine - May 25, 2018 Category: Biotechnology Source Type: research
A proteomic glimpse into the oncogenesis of prostate cancer
Publication date: Available online 24 May 2018 Source:Journal of Applied Biomedicine Author(s): Patrícia Borba Martiny, Diego Duarte Alcoba, Brasil Silva Neto, Paulo Costa Carvalho, Ilma Simoni Brum Prostate cancer (PCa) is the second most frequent cancer in men worldwide. Distinguishing between the nonaggressive and aggressive forms of this disease is difficult, and a means to better characterize molecular patterns that could aid in diagnosis is urgently needed. Here, we compare the proteomic profiles of PCa and benign prostatic hyperplasia (BPH) in an effort to elucidate underlying mechanisms of oncogenesis. We c...
Source: Journal of Applied Biomedicine - May 25, 2018 Category: Biotechnology Source Type: research
In vitro antimalarial activity of synthesized TiO2 nanoparticles using Momordica charantia leaf extract against Plasmodium falciparum
Publication date: Available online 8 May 2018 Source:Journal of Applied Biomedicine Author(s): Pachiyappan Rajiv Gandhi, Chidambaram Jayaseelan, Chinnaperumal Kamaraj, S.R. Radhika Rajasree, Rathinasamy Regina Mary Malaria is a serious global health challenge, and it has infected millions of people worldwide. There is an urgent need for new antimalarial drugs and drug targets for both prophylaxis and chemotherapy. In the present study, we biosynthesized TiO2 nanoparticles (NPs) using the Momordica charantia leaf aqueous extract as a reducing and stabilizing agent. TiO2 NPs were characterized by UV, XRD, FTIR, HRTEM,...
Source: Journal of Applied Biomedicine - May 9, 2018 Category: Biotechnology Source Type: research
Legislation, regulation and policies issues of orphan drugs in developed countries from 2010 to 2016
Publication date: Available online 1 May 2018 Source:Journal of Applied Biomedicine Author(s): Petra Maresova, Blanka Klimova, Kamil Kuca The purpose of this study is to evaluate current legislative policies and regulations with respect to the use of orphan drugs, to emphasize strengths and weaknesses of these policies in the period of 2010–2016 in European countries, especially in relation to the objectives set by the European Council in 2009. This was done by the method of literature search in the databases PubMed, Scopus, ScienceDirect, and Web of Science from 2010 to 2016. The findings show a lack of polices for...
Source: Journal of Applied Biomedicine - May 2, 2018 Category: Biotechnology Source Type: research
Therapeutic potential of rice-derived polyphenols on obesity-related oxidative stress and inflammation
Publication date: Available online 20 March 2018 Source:Journal of Applied Biomedicine Author(s): Esther T. Callcott, Abishek B. Santhakumar, Jixun Luo, Christopher L. Blanchard Global obesity rates are of epidemic proportion. With limited treatments available there is a large demand for therapeutic alternatives. Polyphenols derived from coloured rice varieties may serve as a potential functional food alternative in combating obesity and obesity-related diseases. The antioxidant and anti-inflammatory properties of polyphenols found in coloured rice varieties could have the ability to neutralize oxidative stress and m...
Source: Journal of Applied Biomedicine - March 20, 2018 Category: Biotechnology Source Type: research
Topic application of meloxicam-loaded polymeric nanocapsules as a technological alternative for treatment of the atopic dermatitis in mice
This study investigated the effect of the topical treatment with meloxicam-loaded nanocapsules (M-NC) on symptoms, inflammatory response and oxidative parameters in an atopic dermatitis (AD) model in BALB/c mice. 2,4-Dinitrochlorobenzene (DNCB) was applied to the dorsal skin on days 1–3 for sensitization. Mice were challenged with DNCB on the ear (on days 14–29) and dorsal skin (on days 14, 17, 20, 23, 26, and 29). Treatments with blank nanocapsules (B-NC), free meloxicam (M-F) or M-NC were applied to the backs of the mice from days 14 to 29. On the day 30, skin severity scores and scratching behaviour were determined....
Source: Journal of Applied Biomedicine - March 20, 2018 Category: Biotechnology Source Type: research
Chemopreventive action of non-steroidal anti-inflammatory drugs in 9,10-dimethylbenzanthracene induced lung carcinogenesis in BALB/C mice: Expression of COX-1, COX-2 and Nf- κB
In conclusion, co-administration of etoricoxib and celecoxib has significantly reduced the inflammatory potential of the growing neoplasm in DMBA induced lung cancer in male BALB/C mice. Graphical abstract (Source: Journal of Applied Biomedicine)
Source: Journal of Applied Biomedicine - March 20, 2018 Category: Biotechnology Source Type: research
Fruit waste (peel) as bio-reductant to synthesize silver nanoparticles with antimicrobial, antioxidant and cytotoxic activities
Publication date: Available online 12 March 2018 Source:Journal of Applied Biomedicine Author(s): Annu, Shakeel Ahmed, Gurpreet Kaur, Praveen Sharma, Sandeep Singh, Saiqa Ikram Since last decade, biogenic synthesis of metal or metal-oxide nanoparticles is emerging as an alternative method, which is environment friendly, simple and safe to use. In this article, fruit waste (peel) extract (FWE) of three citrus fruits viz. Citrus limon, Citrus sinensis, and Citrus limetta were used as bio-reductant for green and sustainable synthesis of silver nanoparticles (AgNPs). As-synthesised AgNPs were characterized by using UV...
Source: Journal of Applied Biomedicine - March 13, 2018 Category: Biotechnology Source Type: research
Naringenin alleviates high d-glucose-induced injuries through upregulation of microRNA-30d-5p level in human AC16 cardiac cells
In this study, human AC16 cardiac cells were treated with normal d-glucose and high d-glucose (HG). After transfection with miR-30d-5p inhibitor, Cell Counting Kit-8 (CCK-8) method was used to measure cell viability. Hoechst 33258 staining was performed to observe the morphological changes of nucleus. Enzyme-linked immunosorbent assay (ELISA) was performed to determine the activity of caspase-3. Cell apoptosis was detected by Annexin V-FITC/propidium iodide (PI) staining. Levels of light chain 3 (LC3) including LC3-I and LC3-II as well as nucleoporin p62 (P62) were detected by Western blot. We found that Naringenin treatme...
Source: Journal of Applied Biomedicine - March 2, 2018 Category: Biotechnology Source Type: research
Genetic association of Tumour necrosis factor alpha, Interleukin-18 and Interleukin 1 beta with the risk of coronary artery disease: A case-control study outcome from Kashmir
In this study we investigated the association of TNF-α SNPs [–308G/A (rs1800629), −1031T/C (rs1799964), −863C/A (rs1800630)]; IL-18 [–137G/C (rs187238)] and IL-1β SNPs [+3954C/T (rs1143634), −31C/T (rs1143627), and −511C/T (rs16944)] with coronary artery disease risk in Kashmiri population. A total of 200 cases and 260 controls were recruited in the study. Logistic regression analysis was done to investigate the association between SNPs and CAD risk. In case of TNF-α, the −308G/A-A/A and −863A/A showed an association with disease while −1031T/C was found to have an inverse relation. The IL-18–137G/C ...
Source: Journal of Applied Biomedicine - February 27, 2018 Category: Biotechnology Source Type: research
RIG-1 and MDA5 are the important intracellular sensors against bacteria in septicemia suffering patients
Conclusion Based on the results it seems that MDA5 and RIG-1 are the main intracellular immunity against the bacteria during septicemia and could be considered for their roles in induction of immunity. Graphical abstract (Source: Journal of Applied Biomedicine)
Source: Journal of Applied Biomedicine - February 22, 2018 Category: Biotechnology Source Type: research
JNK inhibitor CC-930 reduces fibrosis in a murine model of Nf1-deficient fracture repair
Publication date: Available online 13 February 2018 Source:Journal of Applied Biomedicine Author(s): Nikita Deo, Jad El-Hoss, Mille Kolind, Kathy Mikulec, Lauren Peacock, David G. Little, Aaron Schindeler Tibial pseudarthrosis often features deficient bone formation, excessive bone resorption, and extensive pathological fibrosis, particularly in individuals with Neurofibromatosis type I (NF1). It was hypothesized that overactive NF1-Ras-JNK signalling may underlie the pathological fibrosis, and that this could be treated via a JNK antagonist. CC-930, a small molecule JNK inhibitor, was trialed in closed fractures ...
Source: Journal of Applied Biomedicine - February 14, 2018 Category: Biotechnology Source Type: research
