Biomechanical evaluation of human lumbar spine in spondylolisthesis
Publication date: February 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 1Author(s): Kamil Joszko, Marek Gzik, Wojciech Wolański, Bożena Gzik-Zroska, Edyta KawlewskaAbstractOne of the least known conditions of the lumbar spine in terms of biomechanics is spondylolisthesis which causes many serious consequences for the patient. This research aimed to perform a mechanical analysis of the origins of spondylolisthesis and its impact on the biomechanics of the lumbar section of the spine. Within the framework of this study, a physiologically model of the lumbar spine was created in the MADYMO software. In the n...
Source: Journal of Applied Biomedicine - July 11, 2018 Category: Biotechnology Source Type: research
DMH4, a VEGFR2 inhibitor, effectively suppresses growth and invasion of lung cancer cells
In this study, we reported that DMH4 can effectively block phosphorylation of VEGFR2 in both H460 and A549 NSCLC cells, which resulted in significant reduction of NSCLC cell viability in a dose-dependent manner, and the growth inhibition (GI50) of DMH4 against H460 and A549 cell lines were 13.27 and 2.75 mm respectively at 24 h. Our further studies demonstrated that DMH4 significantly suppressed migration and invasion of A549 and H460 cells, and induced apoptosis in those cells. Therefore, DMH4 as a small molecular VEGFR2 inhibitor may represent a new valuable drug lead for NSCLC treatment. (Source: Journal of Applied Biomedicine)
Source: Journal of Applied Biomedicine - July 11, 2018 Category: Biotechnology Source Type: research
A novel algorithm for identifying risk factors for rare events: Predicting transient ischemic attack in young patients with low-risk atrial fibrillation
Publication date: February 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 1Author(s): Chieh-Yu Liu, Hui-Chun ChenAbstractIdentification of risk factors for transient ischemic attack (TIA) is crucial for patients with atrial fibrillation (AF). However, identifying risk factors in young patients with low-risk AF is difficult, because the incidence of TIA in such patients is very low, which would result in traditional multiple logistic regression not being able to successfully identify the risk factors in such patients. Therefore, a novel algorithm for identifying risk factors for TIA is necessary. We thus propo...
Source: Journal of Applied Biomedicine - July 11, 2018 Category: Biotechnology Source Type: research
miR-214 and miR-126 were associated with restoration of endothelial function in obesity after exercise and dietary intervention
Publication date: February 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 1Author(s): Shen Wang, Jingwen Liao, Junhao Huang, Honggang Yin, Weiyue Yang, Min HuAbstractObesity would result in increased cardiovascular morbidity including endothelial destruction, and miRNAs are recognized as potent regulators on endothelial function. We therefore explored pivotal miRNAs before and after exercise and dietary intervention in obese adults and examined their potential relationships with selected endothelial function and biomarkers. Obese adults were included in an exercise and dietary intervention training program fo...
Source: Journal of Applied Biomedicine - July 11, 2018 Category: Biotechnology Source Type: research
Proliferation of Toxoplasma gondii (RH strain) is inhibited by the combination of pravastatin and simvastatin with low concentrations of conventional drugs used in toxoplasmosis
Publication date: February 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 1Author(s): Raquel Arruda Sanfelice, Larissa Rodrigues Bosqui, Suelen Santos da Silva, Milena Menegazzo Miranda-Sapla, Luciano Aparecido Panagio, Italmar Teodorico Navarro, Ivete Conchon-Costa, Wander Rogério Pavanelli, Ricardo Sergio Almeida, Idessania Nazareth CostaAbstractToxoplasma gondii, an etiologic agent of toxoplasmosis, is an obligate intracellular parasite, which exhibits an apicoplast organelle which assists in the metabolism of isoprenoids and other pivotal mediators for the parasite survival. Statins are drugs that inhibi...
Source: Journal of Applied Biomedicine - July 11, 2018 Category: Biotechnology Source Type: research
Contents
Publication date: February 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 1Author(s): (Source: Journal of Applied Biomedicine)
Source: Journal of Applied Biomedicine - July 11, 2018 Category: Biotechnology Source Type: research
IL-37 mediates the anti-tumor activity in non-small cell lung cancer through IL-6/STAT3 pathway
Publication date: February 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 1Author(s): Yan-Ming Deng, Hua Zhang, Jian-Miao Liang, Hai-Bing Xian, Ze-Cheng Chen, Yi-Cong Tang, Shuang Yang, Wei-Neng FengAbstractThe occurrence and development of lung cancer is closely related to inflammation. Thus, we conducted the present study to investigate the effects of IL-37 (Interleukin 37), a newly identified anti-inflammatory factor, on non-small cell lung cancer (NSCLC), which accounts for about 85% of all lung cancers. To address the function of IL-37 in NSCLC, we first evaluated IL-37 expression in the human NSCLC tiss...
Source: Journal of Applied Biomedicine - July 11, 2018 Category: Biotechnology Source Type: research
NETosis − Dr. Jekyll and Mr. Hyde in inflammation
Publication date: February 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 1Author(s): Lucia Lauková, Barbora KonečnáAbstractNeutrophils play an important role as the central mediators of the innate immune defence response, providing the first line of host protection. It was shown that these cells can trap and kill various microorganisms through different ways. One of them is a release of neutrophil extracellular traps (NETs) composed of chromatin fibrils and antimicrobial proteins. There is the evidence that the release of NETs does not have only a beneficial effect. NETs can trap and kill microorganisms a...
Source: Journal of Applied Biomedicine - July 11, 2018 Category: Biotechnology Source Type: research
The influence of modulators of acetylcholinesterase on the resistance of mice against soman and on the effectiveness of antidotal treatment of soman poisoning in mice
Publication date: February 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 1Author(s): Jiri Kassa, Jan Korabecny, Eugenie Nepovimova, Daniel JunAbstractThe potency of one reversible inhibitor of acetylcholinesterase (6-chlorotacrine), one reactivator of acetycholinesterase (K027) and their combination to increase the resistance of mice against soman and the efficacy of antidotal treatment of soman-poisoned mice was evaluated. While 6-chlorotacrine was able to markedly protect mice against acute toxicity of soman and the pharmacological pretreatment with 6-chlorotacrine increased the efficacy of antidotal treat...
Source: Journal of Applied Biomedicine - July 11, 2018 Category: Biotechnology Source Type: research
Quantification of uPA in breast tumour tissue extracts by microarray immunoassay: Comparison with ELISA technology
In this study, we evaluated the performances of customized antibody microarray to quantify uPA concentration from reduced extraction solution of breast tumour tissue and compared it with standard ELISA kit. We firstly optimized the elaboration of customized antibody microarray in order to sensitively detect and quantify uPA standard solutions. In the best conditions, we analysed uPA concentration in 16 cytosolic extracts from breast tumour tissue. Results showed that our customized antibody microarray could correctly quantify uPA concentration while consuming 100 times less volume of tumour tissue extraction solution than ...
Source: Journal of Applied Biomedicine - July 11, 2018 Category: Biotechnology Source Type: research
Induction of caspase-mediated apoptosis using Alnus japonica extracts in AGS human gastric carcinoma cells
In this study, A. japonica extracts were evaluated for their in vitro antioxidant potentials and anticancer effects in AGS human gastric carcinoma cell line. The antioxidant properties of A. japonica extracts were evaluated using several biochemical assays, including FRAP (ferric reducing antioxidant power) assay, ABTS (2, 2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)), DPPH (2,2-diphenyl-1-picrylhydrazyl), alkyl and hydroxyl radical scavenging activity assay. Our study showed that ethanol extract of A. japonica (AJE) has a more potent antioxidant activity than its water extract. In addition, AJE extract inhibit...
Source: Journal of Applied Biomedicine - July 11, 2018 Category: Biotechnology Source Type: research
Cell-free nucleic acids in urine as potential biomarkers of kidney disease
Publication date: Available online 9 February 2018Source: Journal of Applied BiomedicineAuthor(s): Marianna Gyurászová, Alexandra Kovalčíková, Janka Bábíčková, Július Hodosy, Ľubomíra TóthováAbstractKidney and uropoetic system diseases represent a major social, economic and health burden. This is mainly because early diagnosis of kidney dysfunction is currently unavailable, since the current markers are often reliably increased only after advanced progression of the renal diseases. Recently, circulating nucleosomes, DNA and numerous forms of RNA have been detected in human biological fluids, such as plasma, u...
Source: Journal of Applied Biomedicine - July 11, 2018 Category: Biotechnology Source Type: research
Antioxidant and anti-inflammatory mechanisms of polyphenols isolated from virgin coconut oil attenuate cadmium-induced oxidative stress-mediated nephrotoxicity and inflammation in rats
ConclusionThe findings showed that VCO polyphenol supplementation protects against Cd-induced nephrotoxicity via its antioxidant and anti-inflammatory mechanisms in rats.Graphical abstract (Source: Journal of Applied Biomedicine)
Source: Journal of Applied Biomedicine - July 11, 2018 Category: Biotechnology Source Type: research
Effect of selected 8-hydroxyquinoline-2-carboxanilides on viability and sulfate metabolism of Desulfovibrio piger
Publication date: Available online 10 February 2018Source: Journal of Applied BiomedicineAuthor(s): Ivan Kushkevych, Monika Vítězová, Jiří Kos, Peter Kollár, Josef JampílekAbstractAn increased number of sulfate-reducing bacteria is often isolated from faeces of patients with gastrointestinal diseases, which can be the cause of the development of bowel inflammation. Frequent use of antibiotics causes the resistance of intestinal microorganisms and ineffective treatment of these diseases. The antimicrobial activity and biological properties of the selected ring-substituted 8-hydroxyquinoline-2-carboxanilides against D...
Source: Journal of Applied Biomedicine - July 11, 2018 Category: Biotechnology Source Type: research
JNK inhibitor CC-930 reduces fibrosis in a murine model of Nf1-deficient fracture repair
Publication date: Available online 13 February 2018Source: Journal of Applied BiomedicineAuthor(s): Nikita Deo, Jad El-Hoss, Mille Kolind, Kathy Mikulec, Lauren Peacock, David G. Little, Aaron SchindelerAbstractTibial pseudarthrosis often features deficient bone formation, excessive bone resorption, and extensive pathological fibrosis, particularly in individuals with Neurofibromatosis type I (NF1). It was hypothesized that overactive NF1-Ras-JNK signalling may underlie the pathological fibrosis, and that this could be treated via a JNK antagonist. CC-930, a small molecule JNK inhibitor, was trialed in closed fractures in ...
Source: Journal of Applied Biomedicine - July 11, 2018 Category: Biotechnology Source Type: research
