Synthesis, antimicrobial activity and physico-chemical properties of some n-alkyldimethylbenzylammonium halides
Publication date: Available online 1 March 2014 Source:Journal of Applied Biomedicine Author(s): Salomé El Hage , Barbora Lajoie , Jean-Luc Stigliani , Aurélie Furiga-Chusseau , Christine Roques , Geneviève Baziard Quaternary ammonium salts (chloride, bromide and iodide; QUATs) with n-alkyl chain lengths between C8 and C18 have been synthesized under optimized experimental conditions. These compounds were tested in vitro for antimicrobial activity against representative bacterial strains (Staphylococcus aureus CIP 4.83, Enterococcus hirae CIP 5855, Pseudomonas aeruginosa CIP 82118, Escherichia coli CIP 53126, ...
Source: Journal of Applied Biomedicine - November 3, 2014 Category: Biotechnology Source Type: research
Expressions of P-glycoprotein in treatment – Resistant Helicobacter pylori patients
Conclusion H. pylori infection induces the expression of P-glycoprotein in the antrum. This study shows a potential protective mechanism which may be exploited through the use of P-glycoprotein inducers, such as rifabutin, may be beneficial in eradication regimens. (Source: Journal of Applied Biomedicine)
Source: Journal of Applied Biomedicine - November 3, 2014 Category: Biotechnology Source Type: research
Tissue profiling by nanogold-mediated mass spectrometry and artificial neural networks in the mouse model of human primary hyperoxaluria 1
Publication date: April 2014 Source:Journal of Applied Biomedicine, Volume 12, Issue 2 Author(s): Jan Houška , Eladia María Peña-Méndez , Juan R. Hernandez-Fernaud , Eduardo Salido , Aleš Hampl , Josef Havel , Petr Vaňhara Correct assessment of tissue histopathology is a necessary prerequisite for any clinical diagnosis. Nowadays, classical methods of histochemistry and immunohistochemistry are complemented by various techniques adopted from molecular biology and bioanalytical chemistry. Mass spectrometry profiling or imaging offered a new level of tissue visualization in the last decade, revealing hidden ...
Source: Journal of Applied Biomedicine - November 3, 2014 Category: Biotechnology Source Type: research
Comparison of the neuroprotective effects of a novel bispyridinium oxime KR-22934 with the oxime K203 and obidoxime in tabun-poisoned male rats
Publication date: April 2014 Source:Journal of Applied Biomedicine, Volume 12, Issue 2 Author(s): Jiří Kassa , Jana Žďárová Karasová , Kamil Kuča , Kamil Musílek , Young-Sik Jung The neuroprotective effects of a novel oxime KR-22934, the oxime K203 and obidoxime in combination with atropine in rats poisoned with tabun at a sublethal dose (200μg/kg i.m.; 80% LD50) were studied. The tabun-induced neurotoxicity was monitored at 24h following tabun challenge using a functional observational battery and an automatic measurement of motor activity. The results indicate that all tabun-poisoned rats treated with ...
Source: Journal of Applied Biomedicine - November 3, 2014 Category: Biotechnology Source Type: research
Investigating the influence of taurine on thiol antioxidant status in Wistar rats with a multi-analytical approach
In this study, we focused on the effect of taurine supplementation on the concentration of antioxidants in blood plasma and erythrocytes of Wistar rats. Taurine was applied in feed mixture in the dosage of 0, 1, 250, 500, 750, 1000, 1500, 2000, 2500, 3000, 3500 and 4000mg/kg. We monitored both enzymatic and non-enzymatic antioxidants – glutathione peroxidase, glutathione reductase, and superoxide dismutase and reduced/oxidized glutathione and metallothionein. Using three different methods 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), Ferric Reducing Antioxidant Power (FRAP) and free radicals, we determi...
Source: Journal of Applied Biomedicine - November 3, 2014 Category: Biotechnology Source Type: research
Modulatory effect of selenium on cell-cycle regulatory genes in the prostate adenocarcinoma cell line
Publication date: April 2014 Source:Journal of Applied Biomedicine, Volume 12, Issue 2 Author(s): Agnieszka Wanda Piastowska-Ciesielska , Małgorzata Gajewska , Waldemar Wagner , Kamila Domińska , Tomasz Ochędalski Epidemiological data indicate that selenium status is inversely connected with cancer risk. Animal and human studies have demonstrated that most inorganic and organic forms of selenium compounds have an anticancer action. This work investigated the impact of organic selenium on the multiple signalling pathways involved in the inhibition of the viability of prostate cancer cells. Prostate adenocarcinom...
Source: Journal of Applied Biomedicine - November 3, 2014 Category: Biotechnology Source Type: research
Role of peroxisome proliferator-activated receptors δ (PPARδ) in rats showing endotoxemic heart failure
Publication date: April 2014 Source:Journal of Applied Biomedicine, Volume 12, Issue 2 Author(s): Ping-Chin Chang , Li-Jen Chen , Juei-Tang Cheng PPARδ-dependent maintenance of inotropic function is mentioned as crucial for cardiomyocytes. However, change of PPARδ in endotoxins-induced cardiac dysfunction is still unclear. The present study is then designed to investigate the changes of PPARδ in rats showing LPS-induced cardiac dysfunction. In the in vivo experiments, adult Wistar rats were treated with intravenous injection of 10mg/kg LPS for 6h. The isolated heart determined in Langendorff apparatus and the hem...
Source: Journal of Applied Biomedicine - November 3, 2014 Category: Biotechnology Source Type: research
Military potential of biological toxins
Publication date: April 2014 Source:Journal of Applied Biomedicine, Volume 12, Issue 2 Author(s): Xiujuan Zhang , Kamil Kuča , Vlastimil Dohnal , Lucie Dohnalová , Qinghua Wu , Chu Wu Toxins are produced by bacteria, plants and animals for defense or for predation. Most of the toxins specifically affect the mammalian nervous system by interfering with the transmission of nerve impulses, and such toxins have the potential for misuse by the military or terrorist organizations. This review discusses the origin, structure, toxicity and symptoms, transmission, mechanism(s) of action, symptomatic treatment of the mos...
Source: Journal of Applied Biomedicine - November 3, 2014 Category: Biotechnology Source Type: research
Elephantopus scaber Linn.: A review on its ethnomedical, phytochemical and pharmacological profile
Publication date: April 2014 Source:Journal of Applied Biomedicine, Volume 12, Issue 2 Author(s): Sachin M. Hiradeve , Vinod D. Rangari Elephantopus scaber Linn., family Asteraceae, is a small herb found in Neotropics, Europe, Asia, Africa and Australia. The plant parts of this herb have been used traditionally for the treatment of a number of diseases in many countries. Sesquiterpene lactones, triterpenoids, steroids, flavonoids and essential oil constituents have been reported from various parts of the plant. The plant has been extensively screened for anticancer activity. Sesquiterpene lactones such as deoxyeleph...
Source: Journal of Applied Biomedicine - November 3, 2014 Category: Biotechnology Source Type: research
Possible hepatocellular toxicity of EGCG under the influence of an inflammagen
In conclusion, EGCG alone can be harmful to liver at high concentrations and this effect may become more pronounced under the influence of an inflammagen. Graphical abstract (Source: Journal of Applied Biomedicine)
Source: Journal of Applied Biomedicine - November 3, 2014 Category: Biotechnology Source Type: research
Diethyldithiocarbamate complexes with metals used as food supplements show different effects in cancer cells
In this study, we demonstrate the effect of synthetic complexes of disulfiram with four various metals (Mn, Fe, Cr and Cu) used as food supplements. These complexes may be spontaneously formed in the blood during the use of disulfiram with divalent metals and thus may suppress the growth of cancer in vivo. The cytotoxic effect of the compounds and the compounds’ ability to inhibit the cellular proteasome were tested in the osteosarcoma cell line U2OS. After 48h, copper and manganese complexes exhibited cytotoxic effect on the cell line, in sharp contrast to both iron and chromium complexes. (Source: Journal of Applied Biomedicine)
Source: Journal of Applied Biomedicine - November 3, 2014 Category: Biotechnology Source Type: research
Chicken immunoglobulins for prophylaxis: Effect of inhaled antibodies on inflammatory parameters in rat airways
Publication date: Available online 5 May 2014 Source:Journal of Applied Biomedicine Author(s): Jana Hadrabová , Barbora Majerová , Věra Černá , Michaela Moserová , Radim Holuša , Václav Mandys , Marie Stiborová , Ilja Stříž , Petr Hodek The prophylaxis against microbial airway infections of cystic fibrosis (CF) patients is an emerging application of chicken yolk antibody (IgY), however, no data on the effect of inhaled IgY have been published yet. Rats were daily (for 28 days) exposed to an aerosol of IgY, ovalbumin (OVA), Fab fragment of IgY, or PBS and their serum, bronchoalveolar lavage (BAL) an...
Source: Journal of Applied Biomedicine - November 3, 2014 Category: Biotechnology Source Type: research
Acute methanol poisonings: Folates administration and visual sequelae
Publication date: Available online 13 May 2014 Source:Journal of Applied Biomedicine Author(s): Sergey Zakharov , Olga Nurieva , Tomas Navratil , Pavel Diblik , Pavel Kuthan , Daniela Pelclova During the outbreak of methanol poisonings in the Czech Republic 2012, we studied the clinical effectiveness of folate therapy in preventing visual damage. Data were obtained from a combined prospective and retrospective study on 79 patients: folinic acid was administered in 28, folic acid in 35; 16 patients received no folates. The groups were comparable by age, time to treatment, laboratory findings, symptoms, and treatme...
Source: Journal of Applied Biomedicine - November 3, 2014 Category: Biotechnology Source Type: research
The prevalence of some Pseudomonas virulence genes related to biofilm formation and alginate production among clinical isolates
Publication date: Available online 23 May 2014 Source:Journal of Applied Biomedicine Author(s): Abdolamir Ghadaksaz , Abbas Ali Imani Fooladi , Hamideh Mahmoodzadeh Hosseini , Mohsen Amin Chronic infections caused by Pseudomonas aeruginosa (P. aeruginosa) isolates are mainly related to resistance to antimicrobials and the production of certain virulence factors. The purpose of this study was twofold: to investigate the prevalence of virulence genes and to study the relationship between biofilm formation/alginate production/antibiotic resistance and the presence of genes associated with biofilm, alginate, flagella a...
Source: Journal of Applied Biomedicine - November 3, 2014 Category: Biotechnology Source Type: research
Scutia buxifolia Reiss inhibit platelet aggregation and alters the activities of enzymes that hydrolyze adenine nucleotides in lymphocytes and platelets
In this study we investigate the effect of aqueous crude extracts of Scutia buxifolia on NTPDase and 5′-nucleotidase activity on platelets and lymphocytes as well as the profile of the platelet aggregation. The effect of the aqueous crude extract obtained from S. buxifolia leaves (SbL) and stem bark (SbS) on enzymatic activities and platelet aggregation was investigated by in vitro tests. The platelets and lymphocytes were exposed to aqueous extracts of S. buxifolia at concentrations 1–200μg/mL in the presence of ATP, ADP, AMP as substrates. The results showed that SbS and SbL potentially inhibited the NTPDase and 5...
Source: Journal of Applied Biomedicine - November 3, 2014 Category: Biotechnology Source Type: research
