Lectin-based analysis of human milk immunoglobulin G fucosylated variants in relation to milk maturation and perinatal risk factors
ConclusionThe highly fucosylated glycovariants of human IgG given with mothers’ milk to immunologically immature newborn seem to be bifunctional molecules with potential therapeutic properties. The analysis of fucosylation status of milk IgG by simple lectin-IgG-ELISA may be helpful to control the immunological quality of milk for milk banking.Graphical abstract (Source: Journal of Applied Biomedicine)
Source: Journal of Applied Biomedicine - July 11, 2018 Category: Biotechnology Source Type: research
RIG-1 and MDA5 are the important intracellular sensors against bacteria in septicemia suffering patients
ConclusionBased on the results it seems that MDA5 and RIG-1 are the main intracellular immunity against the bacteria during septicemia and could be considered for their roles in induction of immunity.Graphical abstract (Source: Journal of Applied Biomedicine)
Source: Journal of Applied Biomedicine - July 11, 2018 Category: Biotechnology Source Type: research
Genetic association of Tumour necrosis factor alpha, Interleukin-18 and Interleukin 1 beta with the risk of coronary artery disease: A case-control study outcome from Kashmir
In this study we investigated the association of TNF-α SNPs [–308G/A (rs1800629), −1031T/C (rs1799964), −863C/A (rs1800630)]; IL-18 [–137G/C (rs187238)] and IL-1β SNPs [+3954C/T (rs1143634), −31C/T (rs1143627), and −511C/T (rs16944)] with coronary artery disease risk in Kashmiri population. A total of 200 cases and 260 controls were recruited in the study. Logistic regression analysis was done to investigate the association between SNPs and CAD risk. In case of TNF-α, the −308G/A-A/A and −863A/A showed an association with disease while −1031T/C was found to have an inverse relation. The IL-18–137G/C ...
Source: Journal of Applied Biomedicine - July 11, 2018 Category: Biotechnology Source Type: research
Naringenin alleviates high d-glucose-induced injuries through upregulation of microRNA-30d-5p level in human AC16 cardiac cells
In this study, human AC16 cardiac cells were treated with normal d-glucose and high d-glucose (HG). After transfection with miR-30d-5p inhibitor, Cell Counting Kit-8 (CCK-8) method was used to measure cell viability. Hoechst 33258 staining was performed to observe the morphological changes of nucleus. Enzyme-linked immunosorbent assay (ELISA) was performed to determine the activity of caspase-3. Cell apoptosis was detected by Annexin V-FITC/propidium iodide (PI) staining. Levels of light chain 3 (LC3) including LC3-I and LC3-II as well as nucleoporin p62 (P62) were detected by Western blot. We found that Naringenin treatme...
Source: Journal of Applied Biomedicine - July 11, 2018 Category: Biotechnology Source Type: research
Fruit waste (peel) as bio-reductant to synthesize silver nanoparticles with antimicrobial, antioxidant and cytotoxic activities
Publication date: Available online 12 March 2018Source: Journal of Applied BiomedicineAuthor(s): Annu, Shakeel Ahmed, Gurpreet Kaur, Praveen Sharma, Sandeep Singh, Saiqa IkramAbstractSince last decade, biogenic synthesis of metal or metal-oxide nanoparticles is emerging as an alternative method, which is environment friendly, simple and safe to use. In this article, fruit waste (peel) extract (FWE) of three citrus fruits viz. Citrus limon, Citrus sinensis, and Citrus limetta were used as bio-reductant for green and sustainable synthesis of silver nanoparticles (AgNPs). As-synthesised AgNPs were characterized by using UV–...
Source: Journal of Applied Biomedicine - July 11, 2018 Category: Biotechnology Source Type: research
Chemopreventive action of non-steroidal anti-inflammatory drugs in 9,10-dimethylbenzanthracene induced lung carcinogenesis in BALB/C mice: Expression of COX-1, COX-2 and Nf-κB
In conclusion, co-administration of etoricoxib and celecoxib has significantly reduced the inflammatory potential of the growing neoplasm in DMBA induced lung cancer in male BALB/C mice.Graphical abstract (Source: Journal of Applied Biomedicine)
Source: Journal of Applied Biomedicine - July 11, 2018 Category: Biotechnology Source Type: research
Topic application of meloxicam-loaded polymeric nanocapsules as a technological alternative for treatment of the atopic dermatitis in mice
This study investigated the effect of the topical treatment with meloxicam-loaded nanocapsules (M-NC) on symptoms, inflammatory response and oxidative parameters in an atopic dermatitis (AD) model in BALB/c mice. 2,4-Dinitrochlorobenzene (DNCB) was applied to the dorsal skin on days 1–3 for sensitization. Mice were challenged with DNCB on the ear (on days 14–29) and dorsal skin (on days 14, 17, 20, 23, 26, and 29). Treatments with blank nanocapsules (B-NC), free meloxicam (M-F) or M-NC were applied to the backs of the mice from days 14 to 29. On the day 30, skin severity scores and scratching behaviour were determined....
Source: Journal of Applied Biomedicine - July 11, 2018 Category: Biotechnology Source Type: research
Therapeutic potential of rice-derived polyphenols on obesity-related oxidative stress and inflammation
Publication date: Available online 20 March 2018Source: Journal of Applied BiomedicineAuthor(s): Esther T. Callcott, Abishek B. Santhakumar, Jixun Luo, Christopher L. BlanchardAbstractGlobal obesity rates are of epidemic proportion. With limited treatments available there is a large demand for therapeutic alternatives. Polyphenols derived from coloured rice varieties may serve as a potential functional food alternative in combating obesity and obesity-related diseases. The antioxidant and anti-inflammatory properties of polyphenols found in coloured rice varieties could have the ability to neutralize oxidative stress and m...
Source: Journal of Applied Biomedicine - July 11, 2018 Category: Biotechnology Source Type: research
Antioxidative effects of aqueous extract of broccoli sprouts against Triazophos induced hepatic and renal toxicity in female Wistar rats
Publication date: May 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 2Author(s): Dharmender Sharma, Gurinder Kaur SanghaAbstractOxidative stress (OS) is a major cause of hepatic and renal disorders, so present investigation was designed to evaluate the antioxidative efficacy of aqueous broccoli extract (BE) via three different doses – 10, 20 and 30 mmol – of glucosinolates against toxic effects of triazophos (TZ), an organophosphorous pesticide, in female rats during 30 days experiment. Six groups of rats were made and were orally intubated with TZ and BE as per experimental design. TZ and BE in...
Source: Journal of Applied Biomedicine - July 11, 2018 Category: Biotechnology Source Type: research
Effect of pramipexole alginate nanodispersion (PAND) on the transgenic Drosophila expressing human alpha synuclein in the brain
Publication date: May 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 2Author(s): Yasir Hasan Siddique, Falaq Naz, Wasi Khan, Smita Jyoti, Braj Raj Singh, Alim Hussain NaqviAbstractIn the present study the effect of pramipexole alginate nanodispersion (PAND) was studied on the transgenic Drosophila exhibiting the PD symptoms. The PD flies were allowed to feed on the diet having PAND at final concentration of 1, 2 and 3 μM. A dose dependent significant delay in the loss of climbing ability and improvement in the activity was observed in PD flies. A dose dependent significant change in the oxidative stress...
Source: Journal of Applied Biomedicine - July 11, 2018 Category: Biotechnology Source Type: research
In vivo assessment of time dependent changes of T2* in medial meniscus under loading at 3T: A preliminary study
Publication date: May 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 2Author(s): Lenka Hornakova, Vladimir Juras, Petr Kubovy, Daniel Hadraba, David Gerych, Pavel Stursa, Xeni Deligianni, Oliver Bieri, Siegfried Trattnig, Karel JelenAbstractDue to the internal structure of the knee joint, the ability to characterize and quantify the dynamic response of the meniscal tissue directly in vivo is highly problematic. The main purpose of this study was to investigate the behaviour of the meniscus under loading conditions. Four healthy young females were included. To obtain T2* values in the meniscus, the vTE sequenc...
Source: Journal of Applied Biomedicine - July 11, 2018 Category: Biotechnology Source Type: research
Antibacterial activity of green silver nanoparticles synthesized from Anogeissus acuminata against multidrug resistant urinary tract infecting bacteria in vitro and host-toxicity testing
Publication date: May 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 2Author(s): Monali P. Mishra, Rabindra N. PadhyAbstractSilver nanoparticles (AgNPs) with aqueous leaf-extract of the timber-yielding plant Anogeissus acuminata were synthesized for in vitro control of pathogenic bacteria. Characterization of AgNPs with ultraviolet-visible spectroscopy, scanning electron microscopy (SEM), energy dispersive X-ray (EDX) spectroscopy, X-Ray diffraction (XRD) study and Fourier transformed infrared spectroscopy (FTIR) study was done for a confirmation of the synthesis. The SEM analysis confirmed that the metal par...
Source: Journal of Applied Biomedicine - July 11, 2018 Category: Biotechnology Source Type: research
Inotodiol protects PC12 cells against injury induced by oxygen and glucose deprivation/restoration through inhibiting oxidative stress and apoptosis
Publication date: May 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 2Author(s): Yan Li, Wenting Zhang, Chun Chen, Chunping Zhang, Jingyu Duan, Huankai Yao, Qunli Wei, Aiguo Meng, Jun ShiAbstractIschemic stroke is a severe cause of disability and death all over the world. To search for effective therapy for ischemic stroke, PC12 cells damaged by oxygenation and glucose deprivation/restoration were employed to assess the protective effects of inotodiol. As a result, inotodiol can improve the cell viability and attenuate the leakage of lactate dehydrogenase. Meanwhile, inotodiol can prevent oxidative stress by ...
Source: Journal of Applied Biomedicine - July 11, 2018 Category: Biotechnology Source Type: research
Caveolin-1 rs4730751 gene polymorphism in kidney allograft recipients
This study enrolled 270 Caucasian deceased – donor renal transplant recipients. We found no statistically significant associations between CAV1 rs4730751 and delayed graft function, acute rejection, or chronic allograft dysfunction, as well as creatinine values at 1–60 months after transplantation, the risk of graft loss, dialysis or death after transplantation, or histopathological changes in kidney allograft biopsies. Our findings do not support an association between the CAV1 gene rs4730751 polymorphism and kidney allograft function in our population.Graphical abstract (Source: Journal of Applied Biomedicine)
Source: Journal of Applied Biomedicine - July 11, 2018 Category: Biotechnology Source Type: research
Nonlinear Heart Rate Variability based artificial intelligence in lung cancer prediction
Publication date: May 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 2Author(s): Reema Shyamsunder Shukla, Yogender AggarwalAbstractLung cancer is uncontrolled growth of cells that occurs due to exposure to smoke, radiation and chemicals, which causes chronic stress and associated with impaired autonomic nervous system. Nonlinear heart rate variability (HRV) analysis has been suggested to uncover the performance status of lung cancer subjects and distinguish them from healthy controls. The present work obtained tachogram from recorded electrocardiogram of 104 lung cancer subjects and 30 healthy controls to ex...
Source: Journal of Applied Biomedicine - July 11, 2018 Category: Biotechnology Source Type: research
