Deterrence in metabolic and biofilms forming activity of Candida species by mycogenic silver nanoparticles
Publication date: Available online 9 March 2017 Source:Journal of Applied Biomedicine Author(s): Sabahat Hamid, Shama Zainab, Rani Faryal, Naeem Ali Candida, a commensal and opportunistic fungal pathogen has been typically known for its biofilm forming ability and device-associated hospital acquired infections in human. The study aimed at exploring the in-vitro anti-biofilm and anti-metabolic activity of AgNPs against C. albicans (n =2), C. tropicalis (n =2) and C. parapsilosis (n =2) isolated from urine samples. Broth dilution method revealed greater than 50% inhibition at 100ppm against Ag NPs in 24h. An overall ...
Source: Journal of Applied Biomedicine - March 8, 2017 Category: Biotechnology Source Type: research
Alpha 2,3- and alpha 2,6-sialylation of human skim milk glycoproteins during milk maturation
Publication date: Available online 1 March 2017 Source:Journal of Applied Biomedicine Author(s): Jolanta Lis-Kuberka, Marta Berghausen-Mazur, Magdalena Orczyk-Pawiłowicz Human milk is a source of glycoconjugates, sialylated forms of which enrich the newborn immature immune system and are crucial for their proper development and well-being. Here, we analyzed the expression of α2,3-/α2,6-sialylated glycotopes on skim milk glycoproteins over lactation. Milk samples were analyzed by lectin-blotting using α2,3- and α2,6- sialic acid specific Maackia amurensis (MAA) and Sambucus nigra (SNA) lectins and sialyl- and asia...
Source: Journal of Applied Biomedicine - February 28, 2017 Category: Biotechnology Source Type: research
Evaluation of cell population data as potential markers of cobalamin and folate deficiency in populations at risk with regard to renal function
Publication date: Available online 1 March 2017 Source:Journal of Applied Biomedicine Author(s): Dragana Totoskovic, Violeta Dopsaj, Jelena Martinovic The purpose of the present study was to investigate neutrophil and monocyte cell population data as novel markers of low cobalamin/folate concentrations and influence of renal function on their usefulness. The study included 284 patients older than 60 years or with dyspepsia symptoms with mean corpuscular volume 80–100fL and C-reactive protein ≤ 50mg/L. Subjects were divided according to renal function and further classified based on cobalamin and folate levels. Neu...
Source: Journal of Applied Biomedicine - February 28, 2017 Category: Biotechnology Source Type: research
The influence of modulators of acetylcholinesterase on the resistance of mice against soman and on the effectiveness of antidotal treatment of soman poisoning in mice
Publication date: Available online 5 February 2017 Source:Journal of Applied Biomedicine Author(s): Jiri Kassa, Jan Korabecny, Eugenie Nepovimova, Daniel Jun The potency of one reversible inhibitor of acetylcholinesterase (6-chlorotacrine), one reactivator of acetycholinesterase (K027) and their combination to increase the resistance of mice against soman and the efficacy of antidotal treatment of soman-poisoned mice was evaluated. While 6-chlorotacrine was able to markedly protect mice against acute toxicity of soman and the pharmacological pretreatment with 6-chlorotacrine increased the efficacy of antidotal treatm...
Source: Journal of Applied Biomedicine - February 5, 2017 Category: Biotechnology Source Type: research
Delta index of the estimated glomerular filtration rate to amend the overestimated Neutrophil Gelatinase-Associated Lipocalin (NGAL) level in systemic inflammatory response syndrome
This study aimed to investigate a delta index of the estimated glomerular filtration rate (δeGFR-index) to amend an overestimated neutrophil gelatinase-associated lipocalin (NGAL) level in systemic inflammatory response syndrome (SIRS). A δeGFR-index was computed, and the NGAL/δeGFR-index was determined. Patients with SIRS exhibited significantly higher levels in NGAL, the δeGFR-index, and high sensitivity C-reactive protein (hsCRP) than non-SIRS inflammatory patients (216ng/mL, 1.32, and 5.64mg/dL vs. 153ng/mL, 1.18, and 2.92mg/dL, respectively, p <0.05). After adjusting for potential confounders, the NGAL/δeG...
Source: Journal of Applied Biomedicine - January 22, 2017 Category: Biotechnology Source Type: research
Lumbrokinase for degradation and reduction of amyloid fibrils associated with amyloidosis
Publication date: Available online 23 January 2017 Source:Journal of Applied Biomedicine Author(s): Sanjay K. Metkar, Agnishwar Girigoswami, Ramachandran Murugesan, Koyeli Girigoswami Amyloidosis is a group of diseases caused by the accumulation of insoluble protein aggregates in different parts of the body. Repeated subcutaneous injection of insulin hormones in diabetic patients leads to localized amyloidosis that is found to be cytotoxic. Thus, agents that can dissociate these aggregates are critically needed. In the present study, insulin amyloid dissociation was demonstrated by the treatment of an enzyme lumbroki...
Source: Journal of Applied Biomedicine - January 22, 2017 Category: Biotechnology Source Type: research
Monoterpene alpha-terpinene induced hepatic oxidative, cytotoxic and genotoxic damage is associated to caspase activation in rats
Publication date: Available online 22 January 2017 Source:Journal of Applied Biomedicine Author(s): Matheus D. Baldissera, Carine F. Souza, Geisa S. Dolci, Thirssa H. Grando, Michele R. Sagrillo, Rodrigo A. Vaucher, Sônia C.A. da Luz, Sérgio O. Silveira, Marta M.M.F. Duarte, Thiago Duarte, Aleksandro S. da Silva, Silvia G. Monteiro The aim of this study was to investigate the occurrence of toxic effects in liver tissue of rats treated with α-terpinene. All treatments were intraperitoneally administered at doses of 0.5, 0.75 and 1.0mLkg−1 during 10days. Liver samples were collected and assessed by histopa...
Source: Journal of Applied Biomedicine - January 21, 2017 Category: Biotechnology Source Type: research
Maturation rates of oocytes and levels of FSHR, LHR and GnRHR of COCs response to FSH concentrations in IVM media for sheep
In conclusion, FSH could accelerate the maturation rate of sheep oocytes and reduce their apoptosis rate, also increase the expression levels of FSHR, LHR and GnRHR mRNAs, and strengthen expressions of FSHR and GnRHR proteins. 10 IU/mL FSH additions were the optimal dose for IVM of sheep oocytes. Graphical abstract (Source: Journal of Applied Biomedicine)
Source: Journal of Applied Biomedicine - January 12, 2017 Category: Biotechnology Source Type: research
Prediction of post-operative survival expectancy in thoracic lung cancer surgery with soft computing
We present many solutions to predict 1-year the post-operative survival expectancy in thoracic lung cancer surgery base on artificial intelligence. We implement multi-layer architecture of SUB- Adaptive neuro fuzzy inference system (MLA-ANFIS) approach with various combinations of multiple input features, neural networks, regression and ELM (extreme learning machine) based on the used thoracic surgery data set with sixteen input features. Our results contribute to the ELM (wave kernel) based on 16 features is more accurate than different proposed methods for predict the post-operative survival expectancy in thoracic lung c...
Source: Journal of Applied Biomedicine - January 8, 2017 Category: Biotechnology Source Type: research
Antibiotic treatment of asymptomatic Ureaplasma infection improves semen parameters in infertile men
Publication date: Available online 23 December 2016 Source:Journal of Applied Biomedicine Author(s): Mohammad Hossein Ahmadi, Akbar Mirsalehian, Mohammad Ali Sadighi Gilani, Abbas Bahador, Malihe Talebi, Reza Salman Yazdi The role of asymptomatic infections caused by Ureaplasma species in male infertility and the efficacy of antibiotics in treatment of this failure is not yet definitely determined. A total of 165 infertile males having abnormal semen parameters (study group) as well as 165 healthy fertile men (control group) were included in this study. Semen samples were taken from all participants and after analy...
Source: Journal of Applied Biomedicine - December 22, 2016 Category: Biotechnology Source Type: research
Electromagnetic field (10Hz, 1mT) protects mesenchymal stem cells from oxygen-glucose deprivation-induced cell death by reducing intracellular Ca2+ and reactive oxygen species
Publication date: Available online 24 November 2016 Source:Journal of Applied Biomedicine Author(s): Jong Hyeok Jung, Jae Young Kim Protective effects of electromagnetic fields (EMFs) against oxygen and glucose deprivation (OGD)-induced human mesenchymal stem cell (MSC) death were studied. Cell survival, intracellular calcium and ROS/RNS levels were measured after culturing MSCs for 3h under OGD with or without EMF exposure. The survival rate of cells cultured under OGD condition was significantly reduced compared to control cells, while cells cultured in OGD with 10Hz/1mT EMF exposure had higher survival ratio than th...
Source: Journal of Applied Biomedicine - November 23, 2016 Category: Biotechnology Source Type: research
The effect of different doses of zinc supplementation on serum element and lactate levels in elite volleyball athletes
Publication date: Available online 5 November 2016 Source:Journal of Applied Biomedicine Author(s): Gunay Eskici, Mehmet Gunay, Abdulkerim Kasim Baltaci, Rasim Mogulkoc The present study aims to examine the effect of different doses of zinc administration on serum element metabolism and fatigue. The study registered 20 female elite athletes. The subjects were divided into two groups in equal numbers. Group supplemented with 220mg/day zinc sulfate. Group supplemented with 440mg/day zinc sulfate. Athletes who were already engaged in their daily training routines were put to a 20-m shuttle run test to create fatigue bef...
Source: Journal of Applied Biomedicine - November 5, 2016 Category: Biotechnology Source Type: research
Molecular mechanisms responsible for programmed cell death-inducing attributes of terpenes from Mesua ferrea stem bark towards human colorectal carcinoma HCT 116 cells
Publication date: Available online 18 October 2016 Source:Journal of Applied Biomedicine Author(s): Muhammad Asif, Majed Ahmed Al-Mansoub, MD Shamsuddin Sultan Khan, Ashwaq Hamid Salem Yehya, Mohammed Oday Ezzat, Chern Ein Oon, Muhammad Atif, Aman Shah Abdul Majid, Amin Malik Shah Abdul Majid The current study explored the in vitro anticancer properties of Mesua ferrea stem bark (SB) extract towards human colon carcinoma HCT116 cells. SB was successively extracted with different solvents using soxhlet apparatus. MTT assay was employed to test toxicity against different cancer and normal cell lines. Active extrac...
Source: Journal of Applied Biomedicine - October 28, 2016 Category: Biotechnology Source Type: research
Hypoglycemic effect of Chrysanthemum morifolium extract on alloxan-induced diabetic mice is associated with peroxisome proliferator-activated receptor α/γ-mediated hepatic glycogen synthesis
Publication date: Available online 19 October 2016 Source:Journal of Applied Biomedicine Author(s): Xiang Shang, Zeng-Yan Zhu, Feng Wang, Jin-Cheng Liu, Jiang-Yun Liu, Mei-Lin Xie Previous studies have indicated that polyphenol-rich Chrysanthemum morifolium extract (CME) may inhibit the formation of hyperlipidemic fatty liver in mice. But there has been no report about therapeutic effect on diabetes mellitus. In the present study, we investigated the action of CME and its potential mechanisms. A mouse model with diabetes mellitus was induced by alloxan. The results showed that after treatment of diabetic mice with ...
Source: Journal of Applied Biomedicine - October 28, 2016 Category: Biotechnology Source Type: research
One-pot and eco-friendly synthesis of silver nanocrystals using Adiantum raddianum: Toxicity against mosquito vectors of medical and veterinary importance
Publication date: Available online 20 October 2016 Source:Journal of Applied Biomedicine Author(s): Marimuthu Govindarajan, Fatma Saeed AlQahtani, Mashael Marzouq AlShebly, Giovanni Benelli Mosquitoes (Diptera: Culicidae) represent a key threat for millions of humans and animals worldwide, since they act as vectors for devastating parasites and pathogens. Eco-friendly control tools are a priority. Plant-mediated biosynthesis of nanoparticles is rapid and cost-effective. Here we biosynthesized poly-dispersed silver nanocrystals (AgNPs) using a cheap aqueous leaf extract of Adiantum raddianum. AgNPs were characterized ...
Source: Journal of Applied Biomedicine - October 28, 2016 Category: Biotechnology Source Type: research
