Electromagnetic field (10Hz, 1mT) protects mesenchymal stem cells from oxygen-glucose deprivation-induced cell death by reducing intracellular Ca2+ and reactive oxygen species

Publication date: Available online 24 November 2016 Source:Journal of Applied Biomedicine Author(s): Jong Hyeok Jung, Jae Young Kim Protective effects of electromagnetic fields (EMFs) against oxygen and glucose deprivation (OGD)-induced human mesenchymal stem cell (MSC) death were studied. Cell survival, intracellular calcium and ROS/RNS levels were measured after culturing MSCs for 3h under OGD with or without EMF exposure. The survival rate of cells cultured under OGD condition was significantly reduced compared to control cells, while cells cultured in OGD with 10Hz/1mT EMF exposure had higher survival ratio than that in equivalent non-exposed cells. This protective effect of EMF was not observed at different frequency/intensity combinations such as 10Hz/0.01mT, 10Hz/0.1mT, 50Hz/1mT and 100Hz/1mT. ROS/RNS levels of cells cultured under OGD conditions significantly increased compared to the control level while 10Hz/1mT EMF alleviated this effect. Intracellular calcium levels in OGD group were higher than control while those in OGD plus 10Hz/1mT EMF group were significantly lower than OGD group. Addition of Ca2+ chelator promoted protective effects of EMF against OGD-induced MSC death. Our results suggest that 10Hz/1mT EMF exposure protects MSCs from OGD-induced cell death and the underlying mechanisms of the protection are reduction of intracellular levels of Ca2+ and ROS/RNS. Graphical abstract
Source: Journal of Applied Biomedicine - Category: Biotechnology Source Type: research