Can we improve clinical results of tonsillectomy using lasers?
In conclusion, all the tested methods offer a safe, uncomplicated alternative to traditional tonsillectomy; however, they do not bring any substantial benefit for the patient in reduction of pain during the postoperative period. Graphical abstract (Source: Journal of Applied Biomedicine)
Source: Journal of Applied Biomedicine - September 9, 2015 Category: Biotechnology Source Type: research
Atorvastatin up-regulates the expression and activity of renal Cytochrome P450 3A2 in diabetic rats
Publication date: Available online 20 August 2015 Source:Journal of Applied Biomedicine Author(s): Hassan Malekinejad, Shahin Alizadeh-Fanalou, Rahim Hobbenaghi, Shirin Rokhsartalb-Azar Effects of atorvastatin on the expression of Cytochrome P450 3A2 and its enzymatic activity in the kidney of diabetic rats were investigated. Diabetes was induced by injection of streptozotocine (50mg/kg, b.w., i.p.) in male Wistar rats. The animals were assigned into four groups including control (C), non-treated diabetic (D), atorvastatin-treated diabetic (AD) and atorvastatin-treated non-diabetic (A) groups. Metabolism of tes...
Source: Journal of Applied Biomedicine - August 21, 2015 Category: Biotechnology Source Type: research
Alzheimer's disease: Potential preventive, non-invasive, intervention strategies in lowering the risk of cognitive decline – A review study
Publication date: Available online 20 August 2015 Source:Journal of Applied Biomedicine Author(s): Blanka Klimova, Kamil Kuca The number of elderly people nowadays is rapidly increasing, especially in the developed countries. According to the Eurostat agency, the number of aging population will double in the following fifty years. This brings about serious problems such as occurrence of diseases connected with aging population. The most frequent aging disease seems to be dementia, out of which the most common is Alzheimer's disease (AD). The purpose of this article is to briefly describe the individual phases of th...
Source: Journal of Applied Biomedicine - August 21, 2015 Category: Biotechnology Source Type: research
Radiosensitizing potential of Plumbagin in B16F1 melanoma tumor cells through mitochondrial mediated programmed cell death
In conclusion, this study demonstrates the radiosensitizing potential of PLB to inhibit the growth of melanoma cells in vitro, which may be attributed to the oxidative stress and DNA damage leading to enhanced mitochondria-mediated programmed cell death resulting in cell death. Also, this study demonstrate the ability of PLB to augment ionizing radiation induced tumor cell kill further warrant the avenue for the development of a clinically useful radiosensitizer. Graphical abstract (Source: Journal of Applied Biomedicine)
Source: Journal of Applied Biomedicine - August 12, 2015 Category: Biotechnology Source Type: research
A comparison of the reactivating and therapeutic efficacy of two novel bispyridinium oximes (K920, K923) with the oxime K203 and trimedoxime in tabun-poisoned rats and mice
Publication date: Available online 5 August 2015 Source:Journal of Applied Biomedicine Author(s): Jiri Kassa, Vendula Sepsova, Anna Horova, Kamil Musilek The potency of two novel oximes (K920, K923) to reactivate tabun-inhibited acetylcholinesterase and to reduce acute toxicity of tabun was compared with the oxime K203 and trimedoxime using in vivo methods. The study determining percentage of reactivation of tabun-inhibited peripheral acetylcholinesterase (diaphragm) and central acetylcholinesterase (brain) in tabun-poisoned rats showed that the reactivating efficacy of both newly developed oximes is lower than...
Source: Journal of Applied Biomedicine - August 7, 2015 Category: Biotechnology Source Type: research
Modulation of ionizing radiation-induced effects by NU7441, KU55933 and VE821 in peripheral blood lymphocytes
In conclusion, KU55933 protected lymphocytes, which might be employed to preserve the immune system during anticancer therapy. NU7441 radiosensitized lymphocytes, thus, undesirable side effects toward immune system could be expected. VE821 showed decrease of γ-H2AX with no radiosensitizing effects in our model likely due to p53 positive status, which underlies the concept of its application in p53 negative environment. Graphical abstract (Source: Journal of Applied Biomedicine)
Source: Journal of Applied Biomedicine - July 27, 2015 Category: Biotechnology Source Type: research
Cetrorelix and Triptorelin active immunization influences follicle development and receptor expressions of ovaries in mice
In conclusion, Cetrorelix and Triptorelin immunization could improve ovarian growth, increase the follicle numbers, enhance dose-dependently the FLD, FTD and FWT, and eventually promote the ovary and follicle development. Cetrorelix decreased expression levels of FSHR and LHR proteins in the ovaries of mice. Triptorelin enhanced expression levels of FSHR and LHR proteins. Triptorelin treatment had more obvious effect than Cetrorelix. Graphical abstract (Source: Journal of Applied Biomedicine)
Source: Journal of Applied Biomedicine - July 14, 2015 Category: Biotechnology Source Type: research
Effect of dietary supplementation of ginger and turmeric rhizomes on ectonucleotidases, adenosine deaminase and acetylcholinesterase activities in synaptosomes from the cerebral cortex of hypertensive rats
In conclusion, this study demonstrated that both rhizomes interfere with the purinergic and cholinergic neurotransmission in cerebral cortex of hypertensive rats. Therefore, we can suggest that both rhizomes exert neuroprotective potential under hypertensive state. Graphical abstract (Source: Journal of Applied Biomedicine)
Source: Journal of Applied Biomedicine - June 26, 2015 Category: Biotechnology Source Type: research
Depletion of androgen receptor (AR) in mesenchymal stem cells (MSCs) inhibits induction of CD4+CD25+FOX3+ regulatory T (Treg) cells via androgen TGF-β interaction
Publication date: Available online 19 June 2015 Source:Journal of Applied Biomedicine Author(s): Abdullah Alawad , Saleh Altuwaijri , Ahmed Aljarbu , Ilona Kryczek , Yuanjie Niu , Fahd A. Al-sobayil , Chawnshang Chang , Ali Bayoumi , Weiping Zou , Volker Rudat , Mohamed Hammad MSCs produce CD4(+)CD25(+)FOX3(+) regulatory T (Treg) cells from activated peripheral blood mononuclear cells (PBMC), T-CD4+ and T-CD8+ cells in vitro and in vivo. Here we investigated whether the deficiency of androgen/AR in MSCs influence Treg induction from total PBMC, splenocytes, CD4+CD25-through AR/TGF-β interaction. Eight to 12...
Source: Journal of Applied Biomedicine - June 20, 2015 Category: Biotechnology Source Type: research
Effect of dietary supplementation of two ginger varieties on ectonucleotidases, adenosine deaminase and acetylcholinesterase activities in synaptosomes from the cerebral cortex of hypertensive rats
In conclusion, this study demonstrated that both gingers interfere with the purinergic and cholinergic neurotransmission in cerebral cortex of hypertensive rats. Therefore, we can suggest that both gingers exert neuroprotective potential under hypertensive state. Graphical abstract (Source: Journal of Applied Biomedicine)
Source: Journal of Applied Biomedicine - June 17, 2015 Category: Biotechnology Source Type: research
Lead compound bearing caffeic scaffold induces EGFR suppression in solid tumor cancer cells
Publication date: Available online 3 June 2015 Source:Journal of Applied Biomedicine Author(s): Chawannuch Mudjupa , Sherif Abdelhamed , Alaa Refaat , Satoru Yokoyama , Ikuo Saiki , Opa Vajragupta A small molecule EGFR inhibitor, 4-(2-(3-(4-(4-(trifluoromethyl)phenyl)thiazol-2-yl)ureido)vinyl)-1,2-phenylene diacetate (CIU1) was designed in silico by using caffeic scaffold as core structure. The designed compound showed anti-proliferative action against different solid tumor cell lines, particularly metastatic breast cancer cells. CIU1 inhibited the growth of EGFR-overexpressing MDA-MB-468 triple-negative breast c...
Source: Journal of Applied Biomedicine - June 4, 2015 Category: Biotechnology Source Type: research
Pharmacological study of a cannabinoid-containing eyedrop formulation in dogs and mice
Publication date: Available online 23 May 2015 Source:Journal of Applied Biomedicine Author(s): Aline Maria Stolf , Daiana Lugarini , Anabel de Oliveira , Adriana Pastre Sereniki , Caroline Capitelli , Douglas Hideki Tanaka , Fabiano Montiani Ferreira , Maria Aparecida Barbato Frazão Vital , Alexandra Acco Cannabinoids have been indicated for the treatment of glaucoma in humans. However, pharmacological studies in other species are lacking. Healthy Beagle dogs were treated with 0.1% cannabinoid eyedrops for 3 or 7 days. Intraocular pressure (IOP) and pupillary diameter (PD) were measured. To evaluate whether ...
Source: Journal of Applied Biomedicine - May 23, 2015 Category: Biotechnology Source Type: research
Enhanced anti-inflammatory benefits of meloxicam-loaded lipid-core nanocapsules in a mouse pleurisy model: A comparative study with a free form drug
Publication date: Available online 23 May 2015 Source:Journal of Applied Biomedicine Author(s): Francine Rodrigues Ianiski , Fernanda D’Avila da Silva , Ethel Antunes Wilhelm , Renata Streck Fernandes , Marta Palma Alves , Marta Maria Mederios Frescura Duarte , Cristiane Luchese The development of new treatments for inflammation continues to be of high interest, since long-acting effect is critical for patients. We investigated whether meloxicam-loaded lipid-core nanocapsules (M-NC) have an anti-inflammatory action superior to a free drug (M-F) on a mouse pleurisy model, by analyzing the time-course of leukocy...
Source: Journal of Applied Biomedicine - May 23, 2015 Category: Biotechnology Source Type: research
The effect of different doses of atropine on gastric myoelectrical activity in fasting experimental pigs
In conclusion, different dose-dependent changes in the EGG pattern were found after moderate to high doses of atropine. Graphical abstract (Source: Journal of Applied Biomedicine)
Source: Journal of Applied Biomedicine - May 23, 2015 Category: Biotechnology Source Type: research
Bioinformatic analysis of miRNAs targeting the key nuclear receptors regulating CYP3A4 gene expression: The challenge of the CYP3A4 “missing heritability” enigma
Publication date: Available online 21 April 2015 Source:Journal of Applied Biomedicine Author(s): Tomas Smutny , Jurjen Duintjer Tebbens , Petr Pavek The expression of the main cytochrome P450 enzyme CYP3A4 displays enormous interindividual variability. Studies addressing the genetic variability of either the CYP3A4 gene itself or its key transcription factors have not found any crucial polymorphism contributing to this variability in expression, a phenomenon is referred to as the “missing heritability of CYP3A4 variability.” Several reports have recently described microRNAs (miRNAs) targeting the CYP3A4 gene an...
Source: Journal of Applied Biomedicine - April 24, 2015 Category: Biotechnology Source Type: research
