Lectin-based analysis of human milk immunoglobulin G fucosylated variants in relation to milk maturation and perinatal risk factors
Conclusion The highly fucosylated glycovariants of human IgG given with mothers’ milk to immunologically immature newborn seem to be bifunctional molecules with potential therapeutic properties. The analysis of fucosylation status of milk IgG by simple lectin-IgG-ELISA may be helpful to control the immunological quality of milk for milk banking. Graphical abstract (Source: Journal of Applied Biomedicine)
Source: Journal of Applied Biomedicine - February 14, 2018 Category: Biotechnology Source Type: research
A survey on applying machine learning techniques for management of diseases
Publication date: Available online 19 January 2018 Source:Journal of Applied Biomedicine Author(s): Enas M.F. El Houby During the past years, the increase in scientific knowledge and the massive data production have caused an exponential growth in databases and repositories. Biomedical domain represents one of the rich data domains. An extensive amount of biomedical data is currently available, ranging from details of clinical symptoms to various types of biochemical data and outputs of imaging devices. Manually extracting biomedical patterns from data and transforming them into machine-understandable knowledge is a dif...
Source: Journal of Applied Biomedicine - February 12, 2018 Category: Biotechnology Source Type: research
Oral melatonin administration improves the objective and subjective sleep quality, increases 6-sulfatoxymelatonin levels and total antioxidant capacity in patients with fibromyalgia
Conclusion The daily intake of melatonin improved the sleep quality, increased the aMT6-s levels and the total antioxidant capacity in patients with fibromyalgia. (Source: Journal of Applied Biomedicine)
Source: Journal of Applied Biomedicine - February 12, 2018 Category: Biotechnology Source Type: research
Effect of gender, age, diet and smoking status on the circadian rhythm of ascorbic acid (vitamin C) of healthy Indians
Conclusion The present observations confirm a definite circadian rhythm in ascorbic acid concentrations with significant effects of age, diet and smoking status in clinical health. Mapping the circadian rhythm of serum ascorbic acid in health can help explore its role in different pathophysiological conditions as pre-disease conditions may be characterized by alterations in the circadian amplitude and/or phase before there is a change in mean value. Graphical abstract (Source: Journal of Applied Biomedicine)
Source: Journal of Applied Biomedicine - February 12, 2018 Category: Biotechnology Source Type: research
Quantification of uPA in breast tumour tissue extracts by microarray immunoassay: Comparison with ELISA technology
In this study, we evaluated the performances of customized antibody microarray to quantify uPA concentration from reduced extraction solution of breast tumour tissue and compared it with standard ELISA kit. We firstly optimized the elaboration of customized antibody microarray in order to sensitively detect and quantify uPA standard solutions. In the best conditions, we analysed uPA concentration in 16 cytosolic extracts from breast tumour tissue. Results showed that our customized antibody microarray could correctly quantify uPA concentration while consuming 100 times less volume of tumour tissue extraction solution than ...
Source: Journal of Applied Biomedicine - February 12, 2018 Category: Biotechnology Source Type: research
Cell-free nucleic acids in urine as potential biomarkers of kidney disease
Publication date: Available online 9 February 2018 Source:Journal of Applied Biomedicine Author(s): Marianna Gyurászová, Alexandra Kovalčíková, Janka Bábíčková, Július Hodosy, Ľubomíra Tóthová Kidney and uropoetic system diseases represent a major social, economic and health burden. This is mainly because early diagnosis of kidney dysfunction is currently unavailable, since the current markers are often reliably increased only after advanced progression of the renal diseases. Recently, circulating nucleosomes, DNA and numerous forms of RNA have been detected in human biological fluids, such as plasma, ...
Source: Journal of Applied Biomedicine - February 12, 2018 Category: Biotechnology Source Type: research
Induction of caspase-mediated apoptosis using Alnus japonica extracts in AGS human gastric carcinoma cells
In this study, A. japonica extracts were evaluated for their in vitro antioxidant potentials and anticancer effects in AGS human gastric carcinoma cell line. The antioxidant properties of A. japonica extracts were evaluated using several biochemical assays, including FRAP (ferric reducing antioxidant power) assay, ABTS (2, 2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)), DPPH (2,2-diphenyl-1-picrylhydrazyl), alkyl and hydroxyl radical scavenging activity assay. Our study showed that ethanol extract of A. japonica (AJE) has a more potent antioxidant activity than its water extract. In addition, AJE extract inhibit...
Source: Journal of Applied Biomedicine - February 12, 2018 Category: Biotechnology Source Type: research
Effect of selected 8-hydroxyquinoline-2-carboxanilides on viability and sulfate metabolism of Desulfovibrio piger
Publication date: Available online 10 February 2018 Source:Journal of Applied Biomedicine Author(s): Ivan Kushkevych, Monika Vítězová, Jiří Kos, Peter Kollár, Josef Jampílek An increased number of sulfate-reducing bacteria is often isolated from faeces of patients with gastrointestinal diseases, which can be the cause of the development of bowel inflammation. Frequent use of antibiotics causes the resistance of intestinal microorganisms and ineffective treatment of these diseases. The antimicrobial activity and biological properties of the selected ring-substituted 8-hydroxyquinoline-2-carboxanilides against ...
Source: Journal of Applied Biomedicine - February 12, 2018 Category: Biotechnology Source Type: research
Antioxidant and anti-inflammatory mechanisms of polyphenols isolated from virgin coconut oil attenuate cadmium-induced oxidative stress-mediated nephrotoxicity and inflammation in rats
Conclusion The findings showed that VCO polyphenol supplementation protects against Cd-induced nephrotoxicity via its antioxidant and anti-inflammatory mechanisms in rats. Graphical abstract (Source: Journal of Applied Biomedicine)
Source: Journal of Applied Biomedicine - February 12, 2018 Category: Biotechnology Source Type: research
NTPDase, 5 ′-nucleotidase and adenosine deaminase activities and purine levels in serum of sickle cell anemia patients
Publication date: Available online 26 December 2017 Source:Journal of Applied Biomedicine Author(s): Lívia G. Castilhos, Stephen A. Adefegha, Pedro H. Doleski, Tatiana M.D. Bertoldo, Cesar Eduardo J. Moritz, Emerson A. Casali, Daniela B.R. Leal Sickle cell anemia (SCA) is a hereditary disorder that is characterized by tendency of hemoglobin molecules within the erythrocytes to polymerize under hypoxia conditions, deform the red cells, and promote vaso-occlusion and endothelial damage. This disease may be associated with the extracellular release of nucleotides, particularly ATP, ADP and adenosine into the circula...
Source: Journal of Applied Biomedicine - December 28, 2017 Category: Biotechnology Source Type: research
Influence of skin permeation enhancers on the transdermal delivery of palonosetron: An in vitro evaluation
This study reveals that the optimized transdermal system with propylene glycol as permeation enhancers can provide effective therapeutic level of palonosetron. Graphical abstract (Source: Journal of Applied Biomedicine)
Source: Journal of Applied Biomedicine - December 21, 2017 Category: Biotechnology Source Type: research
Nonlinear Heart Rate Variability based artificial intelligence in lung cancer prediction
Publication date: Available online 21 December 2017 Source:Journal of Applied Biomedicine Author(s): Reema Shyamsunder Shukla, Yogender Aggarwal (Source: Journal of Applied Biomedicine)
Source: Journal of Applied Biomedicine - December 21, 2017 Category: Biotechnology Source Type: research
Sinomenine inhibits the growth of glioma cells through STAT3 signal pathway
Publication date: Available online 28 November 2017 Source:Journal of Applied Biomedicine Author(s): Yi-ting Wei, Qun-ying Yang, Shuang-bo Fan, Liang-Wang, Dong-Xia Hu, Ping Shuai The present study was designed to examine effects of Sinomenine (SM) on glioma cells growth in vivo and in vitro. Cells growth and apoptosis were detected by MTT assay, TUNEL assay and flow cytometric analysis. In the study, SM treatment led to growth inhibition on a series of glioma cell lines, including U87, U373, U251, Hs683 and T98G. SM prevented U87 growth in the nude mice as well. Inhibitory effects of SM on U87 cells proliferation ...
Source: Journal of Applied Biomedicine - December 15, 2017 Category: Biotechnology Source Type: research
Effect of pramipexole alginate nanodispersion (PAND) on the transgenic Drosophila expressing human alpha synuclein in the brain
Publication date: Available online 1 December 2017 Source:Journal of Applied Biomedicine Author(s): Yasir Hasan Siddique, Falaq Naz, Wasi Khan, Smita Jyoti, Braj Raj Singh, A.H. Naqvi In the present study the effect of pramipexole alginate nanodispersion (PAND) was studied on the transgenic Drosophila exhibiting the PD symptoms. The PD flies were allowed to feed on the diet having PAND at final concentration of 1, 2 and 3μM. A dose dependent significant delay in the loss of climbing ability and improvement in the activity was observed in PD flies. A dose dependent significant change in the oxidative stress markers...
Source: Journal of Applied Biomedicine - December 15, 2017 Category: Biotechnology Source Type: research
Caveolin-1 rs4730751 gene polymorphism in kidney allograft recipients
This study enrolled 270 Caucasian deceased – donor renal transplant recipients. We found no statistically significant associations between CAV1 rs4730751 and delayed graft function, acute rejection, or chronic allograft dysfunction, as well as creatinine values at 1–60 months after transplantation, the risk of graft loss, dialysis or death after transplantation, or histopathological changes in kidney allograft biopsies. Our findings do not support an association between the CAV1 gene rs4730751 polymorphism and kidney allograft function in our population. Graphical abstract (Source: Journal of Applied Biomedicine)
Source: Journal of Applied Biomedicine - December 15, 2017 Category: Biotechnology Source Type: research
