Anticancer potential of bioactive 16-methylheptadecanoic acid methyl ester derived from marine Trichoderma
Publication date: Available online 22 April 2015 Source:Journal of Applied Biomedicine Author(s): Kandasamy Saravanakumar , Raju Vivek , Natarajan Sithranga Boopathy , Li Yaqian , Kandasamy Kathiresan , Jie Chen The present work aimed at purifying the intracellular fungal metabolites, such as 16-methylheptadecanoic acid methyl ester (HDA) and 9,12-octadecadienoic acid (ODA) from marine Trichoderma, Hypocrea lixii TSK8, Hypocrea rufa SKS2 respectively, and investigating their anticancer and antioxidant effects. The two fungal metabolites were tested against two human cancer cell lines, namely oral cancer (KB) and ...
Source: Journal of Applied Biomedicine - April 24, 2015 Category: Biotechnology Source Type: research
Breastfeeding: Antioxidative properties of breast milk
Publication date: Available online 24 April 2015 Source:Journal of Applied Biomedicine Author(s): Carla Matos , Marta Ribeiro , António Guerra Birth is an event that exposes the newborn baby to a high concentration of free radicals (reactive oxygen species (ROS)/reactive nitrogen species (RNS)), which can contribute to several diseases. The balanced equilibrium between ROS/RNS and the antioxidant system of the newborn is a key factor in preventing a plethora of diseases. The antioxidant system involves endogenous and exogenous molecules, from vitamins (A, E, C) to enzymes (glutathione peroxidase, superoxide dismuta...
Source: Journal of Applied Biomedicine - April 24, 2015 Category: Biotechnology Source Type: research
Serum ferritin levels may have a pro-atherosclerotic role in coronary artery disease patients with sleep disordered breathing
Publication date: Available online 7 April 2015 Source:Journal of Applied Biomedicine Author(s): Fernanda S. Hackenhaar , Denis Martinez , Cristini Klein , Tássia M. Medeiros , Paulo V.G. Alabarse , Marco V. Wainstein , Sandro C. Gonçalves , Mara S. Benfato Elevated ferritin levels may lead to oxidative stress, and are associated with coronary artery disease (CAD). Sleep disordered breathing (SDB) is frequently present in atherosclerosis patients, and causes endothelial dysfunction leading to atherosclerotic plaque progression. Hypoxic conditions, such as SDB, may upregulate ferritin. The aim of this study wa...
Source: Journal of Applied Biomedicine - April 7, 2015 Category: Biotechnology Source Type: research
Augmentation of natural killer cell activity in vitro and in vivo by sericin-derived oligopeptides
This study investigated the effects of sericin-derived oligopeptides on natural killer (NK) activity. In vitro exposure of human peripheral blood mononuclear cells with sericin-derived oligopeptides resulted in an augmentation of NK cell activity against K562 target cells and the effects appeared to be dose-related. Experiments designed to examine whether enhanced NK activity was due to direct or indirect activation of NK cells revealed that sericin oligopeptides did not induce activity of purified NK cells, and that sericin oligopeptides augmented NK activity indirectly by inducing the production of IL-2 and IFN-γ cytoki...
Source: Journal of Applied Biomedicine - April 5, 2015 Category: Biotechnology Source Type: research
Self-assembly of carrageenin–CaCO3 hybrid microparticles on bacterial cellulose films for doxorubicin sustained delivery
Publication date: Available online 1 April 2015 Source:Journal of Applied Biomedicine Author(s): Maximiliano L. Cacicedo , Karina Cesca , Valeria E. Bosio , Luismar Porto , Guillermo R. Castro Stereospecific nucleation of mesoporous hybrid microspheres composed of CaCO3 and carrageenan was appended to one side of bacterial cellulose membrane synthesized in static cultures of Gluconacetobacter hansenii to develop an implantable drug delivery device. The synthesis of the hybrid microparticles proceeds by self-assembly mechanism in the presence of calcium and contains tailorable amounts of doxorubicin. However, in th...
Source: Journal of Applied Biomedicine - April 3, 2015 Category: Biotechnology Source Type: research
Fibroblasts isolated from the malignant melanoma influence phenotype of normal human keratinocytes
In this study we tested the effect of fibroblasts isolated from malignant melanoma on phenotype of normal human keratinocytes, especially on their expression of vimentin, cytoskeletal protein weakly expressed in normal human keratinocytes. The co-culture with normal dermal fibroblasts was used for comparison. The results demonstrated the high expression of vimentin in keratinocytes co-cultured with melanoma-associated fibroblasts compared with those co-cultured with normal dermal fibroblasts. These data suggest participation of melanoma-associated fibroblasts-keratinocyte crosstalk in formation of melanoma niche. (Source: ...
Source: Journal of Applied Biomedicine - March 27, 2015 Category: Biotechnology Source Type: research
Coordination compounds in cancer: Past, present and perspectives
Publication date: Available online 27 March 2015 Source:Journal of Applied Biomedicine Author(s): Federica Trudu , Filippo Amato , Petr Vaňhara , Tiziana Pivetta , E.M. Peña-Méndez , Josef Havel Metal-based coordination compounds have been used throughout the history of human medicine to treat various diseases, including cancer. Since the discovery of cisplatin in 1965, a great number of metal coordination complexes, such as platinum, ruthenium, gold or copper have been designed, synthesized and tested in order to develop clinically effective and safe drugs. Currently, many reviews cover applications of cytost...
Source: Journal of Applied Biomedicine - March 27, 2015 Category: Biotechnology Source Type: research
Perinucleolar heterochromatin during the cell differentiation using human leukemic neutrophils as a convenient model
Publication date: Available online 18 March 2015 Source:Journal of Applied Biomedicine Author(s): Karel Smetana , Hana Klamová , Ilona Jirásková , Dana Mikulenková The perinucleolar region represents a special nuclear compartment involved in the cell malignancy and the perinucleolar heterochromatin reflects the presence of silent genes. The present study was undertaken to provide complementary and missing information on the perinucleolar heterochromatin in differentiating neutrophils in the bone marrow of patients with the chronic myeloid leukemia. That lineage is a very convenient model because of the increase...
Source: Journal of Applied Biomedicine - March 19, 2015 Category: Biotechnology Source Type: research
Crocin prevention of anemia-induced changes in structural and functional parameters of mice testes
This study aimed to investigate the effect of phenylhydrazine-induced hemolytic anemia on testicular functions and protective role of crocin in mice. Forty-nine adult male mice were studied in 7 groups. The control mice received normal saline, three groups were treated with 2, 4, and 6mg/100g of phenylhydrazine, and three other groups received 20mg/100g of crocin with phenylhydrazine for 35 days. Then, the blood samples were taken to examine oxidative stress of serum, sperm samples were obtained for IVF testing, and testicle tissue samples were taken for morphological studies. Morphometric results indicated a significant r...
Source: Journal of Applied Biomedicine - March 2, 2015 Category: Biotechnology Source Type: research
Influence of spurious dilution and hyperglycemia on erythrocytes and platelets evaluated with two different hematological analyzers
Publication date: Available online 9 February 2015 Source:Journal of Applied Biomedicine Author(s): Giuseppe Lippi , Ruggero Buonocore , Roberta Musa , Rosalia Aloe , Alessandra Picanza , Silvia Pipitone , Fabian Sanchis-Gomar The integrity of whole blood samples may be compromised by suboptimal collection practices. Therefore, we investigated the influence of spurious hyperglycemia on erythrocytes and platelets, assessed with two hematological analyzers using optical or impedance technique. Three K2EDTA blood specimens were collected from 12 healthy subjects, pooled and divided into four aliquots. The first ali...
Source: Journal of Applied Biomedicine - February 15, 2015 Category: Biotechnology Source Type: research
Up-regulation of orexigenic and down-regulation of anorexigenic neuropeptide gene expression in rat hypothalamus after partial lipectomy
Publication date: Available online 27 January 2015 Source:Journal of Applied Biomedicine Author(s): Agnieszka Dettlaff-Pokora , Tomasz Sledzinski , Julian Swierczynski Leptin down-regulates orexigenic and up-regulates anorexigenic neuropeptide gene expression in hypothalamus. Surgical removal of adipose tissue leads to decrease in circulating leptin concentrations in rats. In the present study, we tested: (a) regulation of neuropeptide gene expression in hypothalamus, (b) food intake, and (c) standard growth rate after removal of adipose tissue in rats. Partial lipectomy caused an approximately 10-fold reduction of ...
Source: Journal of Applied Biomedicine - January 27, 2015 Category: Biotechnology Source Type: research
Free radicals in ultraviolet irradiated melanins and melanin complexes with Cd(II) and Cu(II) – EPR examination
Publication date: Available online 27 January 2015 Source:Journal of Applied Biomedicine Author(s): Magdalena Zdybel , Ewa Chodurek , Barbara Pilawa Effect of UV irradiation on free radicals in different types of melanins and melanin complexes with diamagnetic Cd(II) and paramagnetic Cu(II) was examined by the use of electron paramagnetic resonance (EPR) spectroscopy. The aim of this studies was to compare o-semiquinone free radicals formation in two model eumelanins synthesized from 3,4-dihydroxyphenylalanine (DOPA) and tyrosine in the presence of tyrosinase, and in synthetic pheomelanin, under exposition on ultrav...
Source: Journal of Applied Biomedicine - January 27, 2015 Category: Biotechnology Source Type: research
Estimation of the spinal twisting angle using inertial measurement units during a rod derotation surgery in idiopathic scoliosis patients
This study proposes a method to measure the twisting angle of a rod during a spinal correction surgery in real-time without performing an alignment procedure by using the six-axis data (tri-axis acceleration, tri-axis rate gyro) of the IMU and a Tait-Bryan Euler angle algorithm. To determine whether the twisting angle calculation algorithm offered an improvement, typical procedures described in existing studies were implemented using the same experimental data and were then validated using a three-dimensional motion analysis system (Vicon 460 motion analysis system). The correlation coefficients and the RMSE of the propose...
Source: Journal of Applied Biomedicine - January 15, 2015 Category: Biotechnology Source Type: research
Herbal product silibinin-induced programmed cell death is enhanced by metformin in cervical cancer cells at the dose without influence on nonmalignant cells
Publication date: Available online 4 December 2014 Source:Journal of Applied Biomedicine Author(s): Wen-Shiung Liou , Li-Jen Chen , Ho-Shan Niu , Ting-Ting Yang , Juei-Tang Cheng , Kao-Chang Lin Silibinin is known to display high efficacy against cancer cells and for hepatic protection. Metformin, a well-known antidiabetic agent, has recently been reported to inhibit cancer. In the present study, we investigated the effect of metformin on silibinin-induced programmed cell death in cervical cancer cells (C-33A). MTT assay and Western blot assays were performed to quantify cell viability and the expression of signa...
Source: Journal of Applied Biomedicine - December 5, 2014 Category: Biotechnology Source Type: research
The expression, purification and activity analysis of Francisella tularensis citrulline ureidase in Escherichia coli
Publication date: Available online 2 December 2014 Source:Journal of Applied Biomedicine Author(s): Tingheng Zhu , Sanyu Fang , Weixia Wang , Kun Wang , Zhifeng Cui , Changchun Wang Citrulline ureidase (CTU, EC3.5.1.20) degrades citrulline into ornithine, carbon dioxide, and ammonia. Here, we present the report on expression of recombinant CTU in Escherichia coli. The soluble and active recombinant CTU was expressed in the periplasmic space with the vector pET-22b and the His-tagged CTU was purified with Ni-Affinity Chromatography. The yield of soluble recombinant protein was significantly increased when 1% sorbi...
Source: Journal of Applied Biomedicine - December 4, 2014 Category: Biotechnology Source Type: research
