Cell-free nucleic acids in urine as potential biomarkers of kidney disease
Publication date: Available online 9 February 2018Source: Journal of Applied BiomedicineAuthor(s): Marianna Gyurászová, Alexandra Kovalčíková, Janka Bábíčková, Július Hodosy, Ľubomíra TóthováAbstractKidney and uropoetic system diseases represent a major social, economic and health burden. This is mainly because early diagnosis of kidney dysfunction is currently unavailable, since the current markers are often reliably increased only after advanced progression of the renal diseases. Recently, circulating nucleosomes, DNA and numerous forms of RNA have been detected in human biological fluids, such as plasma, u...
Source: Journal of Applied Biomedicine - July 5, 2018 Category: Biotechnology Source Type: research
Antioxidant and anti-inflammatory mechanisms of polyphenols isolated from virgin coconut oil attenuate cadmium-induced oxidative stress-mediated nephrotoxicity and inflammation in rats
ConclusionThe findings showed that VCO polyphenol supplementation protects against Cd-induced nephrotoxicity via its antioxidant and anti-inflammatory mechanisms in rats.Graphical abstract (Source: Journal of Applied Biomedicine)
Source: Journal of Applied Biomedicine - July 5, 2018 Category: Biotechnology Source Type: research
Effect of selected 8-hydroxyquinoline-2-carboxanilides on viability and sulfate metabolism of Desulfovibrio piger
Publication date: Available online 10 February 2018Source: Journal of Applied BiomedicineAuthor(s): Ivan Kushkevych, Monika Vítězová, Jiří Kos, Peter Kollár, Josef JampílekAbstractAn increased number of sulfate-reducing bacteria is often isolated from faeces of patients with gastrointestinal diseases, which can be the cause of the development of bowel inflammation. Frequent use of antibiotics causes the resistance of intestinal microorganisms and ineffective treatment of these diseases. The antimicrobial activity and biological properties of the selected ring-substituted 8-hydroxyquinoline-2-carboxanilides against D...
Source: Journal of Applied Biomedicine - July 5, 2018 Category: Biotechnology Source Type: research
JNK inhibitor CC-930 reduces fibrosis in a murine model of Nf1-deficient fracture repair
Publication date: Available online 13 February 2018Source: Journal of Applied BiomedicineAuthor(s): Nikita Deo, Jad El-Hoss, Mille Kolind, Kathy Mikulec, Lauren Peacock, David G. Little, Aaron SchindelerAbstractTibial pseudarthrosis often features deficient bone formation, excessive bone resorption, and extensive pathological fibrosis, particularly in individuals with Neurofibromatosis type I (NF1). It was hypothesized that overactive NF1-Ras-JNK signalling may underlie the pathological fibrosis, and that this could be treated via a JNK antagonist. CC-930, a small molecule JNK inhibitor, was trialed in closed fractures in ...
Source: Journal of Applied Biomedicine - July 5, 2018 Category: Biotechnology Source Type: research
Lectin-based analysis of human milk immunoglobulin G fucosylated variants in relation to milk maturation and perinatal risk factors
ConclusionThe highly fucosylated glycovariants of human IgG given with mothers’ milk to immunologically immature newborn seem to be bifunctional molecules with potential therapeutic properties. The analysis of fucosylation status of milk IgG by simple lectin-IgG-ELISA may be helpful to control the immunological quality of milk for milk banking.Graphical abstract (Source: Journal of Applied Biomedicine)
Source: Journal of Applied Biomedicine - July 5, 2018 Category: Biotechnology Source Type: research
RIG-1 and MDA5 are the important intracellular sensors against bacteria in septicemia suffering patients
ConclusionBased on the results it seems that MDA5 and RIG-1 are the main intracellular immunity against the bacteria during septicemia and could be considered for their roles in induction of immunity.Graphical abstract (Source: Journal of Applied Biomedicine)
Source: Journal of Applied Biomedicine - July 5, 2018 Category: Biotechnology Source Type: research
Genetic association of Tumour necrosis factor alpha, Interleukin-18 and Interleukin 1 beta with the risk of coronary artery disease: A case-control study outcome from Kashmir
In this study we investigated the association of TNF-α SNPs [–308G/A (rs1800629), −1031T/C (rs1799964), −863C/A (rs1800630)]; IL-18 [–137G/C (rs187238)] and IL-1β SNPs [+3954C/T (rs1143634), −31C/T (rs1143627), and −511C/T (rs16944)] with coronary artery disease risk in Kashmiri population. A total of 200 cases and 260 controls were recruited in the study. Logistic regression analysis was done to investigate the association between SNPs and CAD risk. In case of TNF-α, the −308G/A-A/A and −863A/A showed an association with disease while −1031T/C was found to have an inverse relation. The IL-18–137G/C ...
Source: Journal of Applied Biomedicine - July 5, 2018 Category: Biotechnology Source Type: research
Naringenin alleviates high d-glucose-induced injuries through upregulation of microRNA-30d-5p level in human AC16 cardiac cells
In this study, human AC16 cardiac cells were treated with normal d-glucose and high d-glucose (HG). After transfection with miR-30d-5p inhibitor, Cell Counting Kit-8 (CCK-8) method was used to measure cell viability. Hoechst 33258 staining was performed to observe the morphological changes of nucleus. Enzyme-linked immunosorbent assay (ELISA) was performed to determine the activity of caspase-3. Cell apoptosis was detected by Annexin V-FITC/propidium iodide (PI) staining. Levels of light chain 3 (LC3) including LC3-I and LC3-II as well as nucleoporin p62 (P62) were detected by Western blot. We found that Naringenin treatme...
Source: Journal of Applied Biomedicine - July 5, 2018 Category: Biotechnology Source Type: research
Fruit waste (peel) as bio-reductant to synthesize silver nanoparticles with antimicrobial, antioxidant and cytotoxic activities
Publication date: Available online 12 March 2018Source: Journal of Applied BiomedicineAuthor(s): Annu, Shakeel Ahmed, Gurpreet Kaur, Praveen Sharma, Sandeep Singh, Saiqa IkramAbstractSince last decade, biogenic synthesis of metal or metal-oxide nanoparticles is emerging as an alternative method, which is environment friendly, simple and safe to use. In this article, fruit waste (peel) extract (FWE) of three citrus fruits viz. Citrus limon, Citrus sinensis, and Citrus limetta were used as bio-reductant for green and sustainable synthesis of silver nanoparticles (AgNPs). As-synthesised AgNPs were characterized by using UV–...
Source: Journal of Applied Biomedicine - July 5, 2018 Category: Biotechnology Source Type: research
Chemopreventive action of non-steroidal anti-inflammatory drugs in 9,10-dimethylbenzanthracene induced lung carcinogenesis in BALB/C mice: Expression of COX-1, COX-2 and Nf-κB
In conclusion, co-administration of etoricoxib and celecoxib has significantly reduced the inflammatory potential of the growing neoplasm in DMBA induced lung cancer in male BALB/C mice.Graphical abstract (Source: Journal of Applied Biomedicine)
Source: Journal of Applied Biomedicine - July 5, 2018 Category: Biotechnology Source Type: research
Topic application of meloxicam-loaded polymeric nanocapsules as a technological alternative for treatment of the atopic dermatitis in mice
This study investigated the effect of the topical treatment with meloxicam-loaded nanocapsules (M-NC) on symptoms, inflammatory response and oxidative parameters in an atopic dermatitis (AD) model in BALB/c mice. 2,4-Dinitrochlorobenzene (DNCB) was applied to the dorsal skin on days 1–3 for sensitization. Mice were challenged with DNCB on the ear (on days 14–29) and dorsal skin (on days 14, 17, 20, 23, 26, and 29). Treatments with blank nanocapsules (B-NC), free meloxicam (M-F) or M-NC were applied to the backs of the mice from days 14 to 29. On the day 30, skin severity scores and scratching behaviour were determined....
Source: Journal of Applied Biomedicine - July 5, 2018 Category: Biotechnology Source Type: research
Therapeutic potential of rice-derived polyphenols on obesity-related oxidative stress and inflammation
Publication date: Available online 20 March 2018Source: Journal of Applied BiomedicineAuthor(s): Esther T. Callcott, Abishek B. Santhakumar, Jixun Luo, Christopher L. BlanchardAbstractGlobal obesity rates are of epidemic proportion. With limited treatments available there is a large demand for therapeutic alternatives. Polyphenols derived from coloured rice varieties may serve as a potential functional food alternative in combating obesity and obesity-related diseases. The antioxidant and anti-inflammatory properties of polyphenols found in coloured rice varieties could have the ability to neutralize oxidative stress and m...
Source: Journal of Applied Biomedicine - July 5, 2018 Category: Biotechnology Source Type: research
Antioxidative effects of aqueous extract of broccoli sprouts against Triazophos induced hepatic and renal toxicity in female Wistar rats
Publication date: May 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 2Author(s): Dharmender Sharma, Gurinder Kaur SanghaAbstractOxidative stress (OS) is a major cause of hepatic and renal disorders, so present investigation was designed to evaluate the antioxidative efficacy of aqueous broccoli extract (BE) via three different doses – 10, 20 and 30 mmol – of glucosinolates against toxic effects of triazophos (TZ), an organophosphorous pesticide, in female rats during 30 days experiment. Six groups of rats were made and were orally intubated with TZ and BE as per experimental design. TZ and BE in...
Source: Journal of Applied Biomedicine - July 5, 2018 Category: Biotechnology Source Type: research
Effect of pramipexole alginate nanodispersion (PAND) on the transgenic Drosophila expressing human alpha synuclein in the brain
Publication date: May 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 2Author(s): Yasir Hasan Siddique, Falaq Naz, Wasi Khan, Smita Jyoti, Braj Raj Singh, Alim Hussain NaqviAbstractIn the present study the effect of pramipexole alginate nanodispersion (PAND) was studied on the transgenic Drosophila exhibiting the PD symptoms. The PD flies were allowed to feed on the diet having PAND at final concentration of 1, 2 and 3 μM. A dose dependent significant delay in the loss of climbing ability and improvement in the activity was observed in PD flies. A dose dependent significant change in the oxidative stress...
Source: Journal of Applied Biomedicine - July 5, 2018 Category: Biotechnology Source Type: research
In vivo assessment of time dependent changes of T2* in medial meniscus under loading at 3T: A preliminary study
Publication date: May 2018Source: Journal of Applied Biomedicine, Volume 16, Issue 2Author(s): Lenka Hornakova, Vladimir Juras, Petr Kubovy, Daniel Hadraba, David Gerych, Pavel Stursa, Xeni Deligianni, Oliver Bieri, Siegfried Trattnig, Karel JelenAbstractDue to the internal structure of the knee joint, the ability to characterize and quantify the dynamic response of the meniscal tissue directly in vivo is highly problematic. The main purpose of this study was to investigate the behaviour of the meniscus under loading conditions. Four healthy young females were included. To obtain T2* values in the meniscus, the vTE sequenc...
Source: Journal of Applied Biomedicine - July 5, 2018 Category: Biotechnology Source Type: research
