Monoterpene alpha-terpinene induced hepatic oxidative, cytotoxic and genotoxic damage is associated to caspase activation in rats

Publication date: Available online 22 January 2017 Source:Journal of Applied Biomedicine Author(s): Matheus D. Baldissera, Carine F. Souza, Geisa S. Dolci, Thirssa H. Grando, Michele R. Sagrillo, Rodrigo A. Vaucher, Sônia C.A. da Luz, Sérgio O. Silveira, Marta M.M.F. Duarte, Thiago Duarte, Aleksandro S. da Silva, Silvia G. Monteiro The aim of this study was to investigate the occurrence of toxic effects in liver tissue of rats treated with α-terpinene. All treatments were intraperitoneally administered at doses of 0.5, 0.75 and 1.0mLkg−1 during 10days. Liver samples were collected and assessed by histopathological analysis, caspases −1, −3 and −8 assay, biomarkers of hepatic damage and determination of oxidant/antioxidant status (thiobarbituric acid-reactive substances (TBARS), catalase (CAT), superoxide dismutase (SOD), reactive oxygen species (ROS), glutathione S-transferase (GST) and glutathione peroxidase (GPx)). Additionally, the cytotoxic and genotoxic effects were evaluated by comet assay. An increase was observed on TBARS levels and GPx activity on the hepatic tissue. Instead, CAT and SOD activities decreased in rats treated with a dose of 1.0mLkg−1 of α-terpinene. Concomitantly, ROS levels increased and GST levels decreased in rats treated with α-terpinene at doses of 0.5, 0.75 and 1.0mLkg−1. Also, there was an increase in frequency of damage, damage index and caspases, while cell viability decreased in rats treated with α-terpinene. A...
Source: Journal of Applied Biomedicine - Category: Biotechnology Source Type: research