Investigating the influence of taurine on thiol antioxidant status in Wistar rats with a multi-analytical approach

In this study, we focused on the effect of taurine supplementation on the concentration of antioxidants in blood plasma and erythrocytes of Wistar rats. Taurine was applied in feed mixture in the dosage of 0, 1, 250, 500, 750, 1000, 1500, 2000, 2500, 3000, 3500 and 4000mg/kg. We monitored both enzymatic and non-enzymatic antioxidants – glutathione peroxidase, glutathione reductase, and superoxide dismutase and reduced/oxidized glutathione and metallothionein. Using three different methods 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), Ferric Reducing Antioxidant Power (FRAP) and free radicals, we determined antioxidant capacity. In addition, we monitored levels of uric acid and glucose. Our results revealed significant changes in both enzymatic and non-enzymatic parameters with the increasing taurine supplementation.

Source: Journal of Applied Biomedicine - November 3, 2014 Category: Biotechnology Source Type: research