Systemic oxidative stress in victims of Bothrops snakebites

Publication date: Available online 1 December 2014 Source:Journal of Applied Biomedicine Author(s): Juliana de Ornellas Strapazzon , Eduardo Benedetti Parisotto , Ana Maria Moratelli , Thais Regina Garlet , Juliana Bastos , Ivan Ricardo Zimermann , Marlene Zanin , Rodrigo Fagundez , Manuel Rosa de Oliveira Lino , Tânia Silvia Fröde , Danilo Wilhelm-Filho The genus Bothrops is responsible for approximately 90% of snakebites in Brazil. In the present study biomarkers of oxidative stress (OS) were evaluated in the blood of victims of snakebites from Bothrops jararaca and Bothrops jararacussu. Patient monitoring started from the emergency entrance at the hospital up to 30 days, groups divided as follows: time 0 (t0), 24hours (t24h), 7 days (t7d) and 30 days (t30d). The activities of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione S-transferase (GST), and myeloperoxidase (MPO), as well as the contents of reduced glutathione (GSH), vitamin E, lipid peroxidation (TBARS), protein carbonyls (PC) were examined in blood. Initial determinations revealed increased CAT, GR and GPx activities and decreased SOD and GST activities together with the depletion of GSH contents, while markers of oxidative damage showed increased TBARS levels and decreased PC concentrations in victims of snakebite compared to controls (blood donors). Regarding the temporal effect, no statistical differences among the groups were det...
Source: Journal of Applied Biomedicine - Category: Biotechnology Source Type: research