Nitrite contamination in hypotensive preparations of dinitrosyl iron complexes with glutathione

Publication date: 2013 Source:Journal of Applied Biomedicine, Volume 11, Issue 4 Author(s): Rostislav Romanovich Borodulin , Vyacheslav Olegovich Shvydkiy , Lyudmila Nikolaevna Kubrina , Vasak Dzhanibekovich Mikoyan , Natalya Ilinishna Zaitseva , Tatyana Evgenjevna Sporyagina , Sergey Alexandrovich Kryuchkov , Anatoly Fjodorovich Vanin The content of nitrite admixture in preparations of dinitrosyl iron complexes (DNIC) with glutathione synthesized by treatment of aqueous solutions of Fe2+ + glutathione with gaseous NO (complex 1) or by mixing solutions of S-nitrosoglutathione (GS-NO) with solutions of Fe2+ + glutathione (complex 2) was determined using the Griess method and HPLC as well as from the level of HNO2 formed upon interaction of gaseous NO with acidified distilled water. In both preparations, DNIC were predominantly represented by the binuclear form (B-DNIC). In complex 1, the appearance of nitrite in DNIC solutions was induced by nitrogen dioxide present in gaseous NO; its interaction with NO gives an adduct, which is further hydrolyzed to nitrite in aqueous solutions. In complex 2, the presence of nitrite admixture could appear in the presence of nitrite non-incorporated into GS-NO synthesized by mixing glutathione and nitrite in acid media. The per cent content of nitrite (with respect to the total content of complex 1) was 6%, whereas in complex 2 it was as low as 0.4%. Such a low level of nitrite contamination in the course of conventional synthesis...

Source: Journal of Applied Biomedicine - November 3, 2014 Category: Biotechnology Source Type: research