The impact of sex steroid agonists and antagonists on folliculogenesis in the neonatal porcine ovary via cell proliferation and apoptosis
The objective of the study was to examine the effects of androgen and estrogen agonists or antagonists on the follicle formation, ovarian cell proliferation and apoptosis as well as plasma steroid concentration in neonatal pigs. Piglets were injected with testosterone propionate (TP, 20 mg/kg bw), flutamide (FLU, 50 mg/kg bw), 4-tert-octylphenol (OP, 100 mg/kg bw), ICI 182,780 (ICI, 400 μg/kg bw), methoxychlor (MXC, 100 mg/kg bw) or corn oil (CTR, controls) between postnatal Days 1 and 10 (n = 4/group). (Source: Theriogenology)
Source: Theriogenology - February 9, 2018 Category: Genetics & Stem Cells Authors: Katarzyna Knapczyk-Stwora, Malgorzata Grzesiak, Renata E. Ciereszko, Elzbieta Czaja, Marek Koziorowski, Maria Slomczynska Source Type: research
Enhanced contraception of canine zona pellucida 3 DNA vaccine via targeting DEC-205 in mice
In this study, we designed a canine ZP3 (CZP3) DNA vaccine through targeting DEC-205 (named as pcD-scFv-CZP3c) and investigated its contraceptive effect in mice. Female BALB/c mice were intramuscularly immunized 3 times at 2 weeks intervals. After immunization, humoral and cellular immune responses were detected by ELISA and flow cytometry. The results showed that pcD-CZP3 and pcD-scFv-CZP3c induced CZP3-specific Ab responses both in serum and vaginal secretions compared to pcDNA3.1. (Source: Theriogenology)
Source: Theriogenology - February 9, 2018 Category: Genetics & Stem Cells Authors: Ying Wang, Beibei Zhang, Jinyao Li, Adila Aipire, Yijie Li, Fuchun Zhang Source Type: research
Effect of sodium alginate on mouse ovary vitrification
The survival rate of vitrified-thawed ovarian tissues after autotransplantation still needs to be improved. Therefore finding an ideal cryoprectant to reduce the damage to ovaries that caused by vitrification will pave the way for application of ovary cryopreservation on clinics. Experiments were conducted to investigate the effect of sodium alginate in cryoprotectant solution on mouse ovaries during the vitrification process. The ovaries obtained from 6-weeks old CD1 were assigned into six groups from A to F. (Source: Theriogenology)
Source: Theriogenology - February 9, 2018 Category: Genetics & Stem Cells Authors: Gang Liu, Shubin Li, Haihan Yuan, Mengrui Hao, Wurihan, Zhizhong Yun, Jie Zhao, Yuzhen Ma, Yanfeng Dai Source Type: research
The impact of sex steroid agonists and antagonists on folliculogenesis in the neonatal porcine ovary via cell proliferation and apoptosis
The objective of the study was to examine the effects of androgen and estrogen agonists or antagonists on the follicle formation, ovarian cell proliferation and apoptosis as well as plasma steroid concentration in neonatal pigs. Piglets were injected with testosterone propionate (TP, 20 mg/kg bw), flutamide (FLU, 50 mg/kg bw), 4-tert-octylphenol (OP, 100 mg/kg bw), ICI 182,780 (ICI, 400 μg/kg bw), methoxychlor (MXC, 100 mg/kg bw) or corn oil (CTR, controls) between postnatal Days 1 and 10 (n = 4/group). (Source: Theriogenology)
Source: Theriogenology - February 9, 2018 Category: Genetics & Stem Cells Authors: Katarzyna Knapczyk-Stwora, Malgorzata Grzesiak, Renata E. Ciereszko, Elzbieta Czaja, Marek Koziorowski, Maria Slomczynska Source Type: research
Effect of consecutive re-synchronization protocols on pregnancy rate in buffalo (Bubalus bubalis) heifers out of the breeding season
The combined effect of six consecutive timed artificial inseminations (TAIs) on pregnancy rates, following two different synchronization protocols on buffalo heifers, over a period of seven months typically characterized by low breeding performances, were investigated in this study. A total of 2189 TAIs were performed on 1463 buffalo heifers within a large buffalo farm in the south of Italy. Individual animals were allowed to undergo synchronization protocol (either a slightly modified Ovsynch or Progesterone treatment) and TAI until establishment of pregnancy or else for not more than six consecutive times. (Source: Theriogenology)
Source: Theriogenology - February 8, 2018 Category: Genetics & Stem Cells Authors: Gianluca Neglia, Massimo Capuano, Anna Balestrieri, Roberta Cimmino, Francesco Iannaccone, Francesco Palumbo, Giorgio A. Presicce, Giuseppe Campanile Source Type: research
Intracytoplasmic sperm injection (ICSI) of in vitro matured oocytes with stored epididymal spermatozoa in camel (Camelus dromedarius): Effect of exogenous activation on in vitro embryo development
Experiments were conducted to investigate the development of in vitro matured camel oocytes after their intra-cytoplasmic sperm injection (ICSI) with epididymal sperm collected from slaughtered male camels. Ovaries and testes were collected from a local slaughterhouse in normal saline solution (NSS) at 37 °C and on ice (0–1 °C), respectively. Cumulus-o ocyte complexes (COCs) aspirated from the follicles were randomly distributed to 4-well culture plates (20–25 COCs/well) containing 500 μL of maturation medium and cultured at 38.5 °C in an atmosphere of 5% CO2 in air for about 30 h. (Source: Theriogenology)
Source: Theriogenology - February 8, 2018 Category: Genetics & Stem Cells Authors: Nisar A. Wani, Seungbum Hong Source Type: research
Effect of consecutive re-synchronization protocols on pregnancy rate in buffalo (Bubalus bubalis) heifers out of the breeding season
The combined effect of six consecutive timed artificial inseminations (TAIs) on pregnancy rates, following two different synchronization protocols on buffalo heifers, over a period of seven months typically characterized by low breeding performances, were investigated in this study. A total of 2189 TAIs were performed on 1463 buffalo heifers within a large buffalo farm in the south of Italy. Individual animals were allowed to undergo synchronization protocol (either a slightly modified Ovsynch or Progesterone treatment) and TAI until establishment of pregnancy or else for not more than six consecutive times. (Source: Theriogenology)
Source: Theriogenology - February 8, 2018 Category: Genetics & Stem Cells Authors: Gianluca Neglia, Massimo Capuano, Anna Balestrieri, Roberta Cimmino, Francesco Iannaccone, Francesco Palumbo, Giorgio A. Presicce, Giuseppe Campanile Source Type: research
Intra cytoplasmic sperm injection (ICSI) of in vitro matured oocytes with stored epididymal spermatozoa in camel (Camelus dromedarius): Effect of exogenous activation on in vitro embryo development
Experiments were conducted to investigate the development of in vitro matured camel oocytes after their intra-cytoplasmic sperm injection (ICSI) with epididymal sperm collected from slaughtered male camels. Ovaries and testes were collected from a local slaughterhouse in normal saline solution (NSS) at 37 °C and on ice (0–1 °C), respectively. Cumulus-oocyte complexes (COCs) aspirated from the follicles were randomly distributed to 4-well culture plates (20–25 COCs/well) containing 500 μL of maturation medium and cultured at 38.5 °C in an atmosphere of 5% CO2 in air for about 30 h. (Source: Theriogenology)
Source: Theriogenology - February 8, 2018 Category: Genetics & Stem Cells Authors: Nisar A. Wani, Seungbum Hong Source Type: research
Constituents of neutrophil extracellular traps induce in vitro collagen formation in mare endometrium
Neutrophil extracellular traps (NETs) are DNA complexes carrying nuclear and cytoplasmic proteins, such as elastase (ELA), cathepsin-G (CAT) and myeloperoxidase (MPO). Mare endometrosis is a chronic degenerative process characterized by excessive collagen in endometrium. While NETs fight bacteria that cause endometritis, they may trigger endometrial fibrogenesis. The aim was to evaluate the in vitro effect of some NETs components on mare endometrial fibrogenesis and determine its relationship with histopathology or estrous cycle. (Source: Theriogenology)
Source: Theriogenology - February 6, 2018 Category: Genetics & Stem Cells Authors: Maria Rosa Rebord ão, Ana Amaral, Karolina Lukasik, Anna Szóstek-Mioduchowska, Pedro Pinto-Bravo, António Galvão, Dariusz J. Skarzynski, Graça Ferreira-Dias Source Type: research
Constituents of neutrophil extracellular traps induce in vitro collagen formation in mare endometrium
Neutrophil extracellular traps (NETs) are DNA complexes carrying nuclear and cytoplasmic proteins, such as elastase (ELA), cathepsin-G (CAT) and myeloperoxidase (MPO). Mare endometrosis is a chronic degenerative process characterized by excessive collagen in endometrium. While NETs fight bacteria that cause endometritis, they may trigger endometrial fibrogenesis. The aim was to evaluate the in vitro effect of some NETs components on mare endometrial fibrogenesis and determine its relationship with histopathology or estrous cycle. (Source: Theriogenology)
Source: Theriogenology - February 6, 2018 Category: Genetics & Stem Cells Authors: Maria Rosa Rebord ão, Ana Amaral, Karolina Lukasik, Anna Szóstek-Mioduchowska, Pedro Pinto-Bravo, António Galvão, Dariusz J. Skarzynski, Graça Ferreira-Dias Source Type: research
Effect of different chorionic gonadotropins on final growth of the dominant follicle in Bos indicus cows
The aim of this study was to evaluate the effect of eCG or hCG on the final growth of the dominant follicle in Nelore (Bos indicus) cows submitted to fixed-time AI (FTAI). Eighty-four lactating cows with body condition score (BCS) of 2.9 (range 1 –5) were used. At a random day of the estrous cycle (D0) cows received 2 mg estradiol benzoate and a reused intravaginal progesterone device (1.9 g). At D8, when the device was removed, 0.5 mg cloprostenol and 1 mg estradiol cypionate was given i.m., and cows were randomly assigned to rece ive on D8 one of the following treatments: Control (no treatment; n = 17), eCG...
Source: Theriogenology - February 5, 2018 Category: Genetics & Stem Cells Authors: Alexandre B. Prata, J éssica N. Drum, Leonardo F. Melo, Estela R. Araujo, Roberto Sartori Source Type: research
Effect of multiple low-dose PGF2 α injections on the mature corpus luteum in non-pregnant bitches
This study investigated molecular regulation in the canine corpus luteums/corpora lutea (CL) following multiple low-dose prostaglandin F2 alpha (PGF2 α) injections in non-pregnant bitches around 30–35 days after ovulation. The CL were obtained by ovariohysterectomy 1 h after the last PGF2α injection. The subjects were divided into the following groups: control (no PGF2α injection, n = 4), one PGF2α injection (injection at 0 h, 1PGF, n = 4), two PGF2α injection (injection at 0 and 8 h, 2PGF, n = 4), and three PGF2α injection (injection at 0, 8 and 24 h, 3PGF, n = 4). (Source: Theriogenology)
Source: Theriogenology - February 4, 2018 Category: Genetics & Stem Cells Authors: Eyyup H. Ucar, Hayrettin Cetin, Mehmet O. Atli Source Type: research
