Synchronization treatments previous to natural breeding anticipate and improve the pregnancy rate of postpartum primiparous beef cows
Timed AI has become a potential tool to bypass postpartum acyclicity, yet only a small percentage of the world bovine herd is inseminated. Most females are still subjected to bull mating; therefore, the frequent occurrence of postpartum anestrus may compromise their reproductive efficiency. Thus, the aim of this study was to develop an approach that allows the early conception of postpartum primiparous beef cows that are exposed to natural breeding (NB). For this purpose, 350 primiparous Nelore cows 35 –65 d postpartum were allocated into three groups: Control (no hormonal treatment), TNB (hormonal protocol for timed-NB...
Source: Theriogenology - April 4, 2018 Category: Genetics & Stem Cells Authors: R.M. Ferreira, T.L. Conti, R.L. Gon çalves, L.A. Souto, J.N.S. Sales, M.F. Sá Filho, F.M. Elliff, P.S. Baruselli Source Type: research
Differential release of cell-signaling metabolites by male and female bovine embryos cultured in vitro
Male and female early bovine embryos show dimorphic transcription that impacts metabolism. Individual release of metabolites was examined in a 24h single culture medium from Day-6 male and female morulae that developed to Day-7 expanded blastocysts. Embryos were produced in vitro, fertilized with a single bull and cultured in SOFaaci+6 g/L BSA. The embryonic sex was identified (amelogenin gene amplification). Embryos (N = 10 males and N = 10 females) and N = 6 blank samples (i.e. SOFaaci+6 g/L BSA incubated with no embryos) were collected from 3 replicate s. (Source: Theriogenology)
Source: Theriogenology - April 4, 2018 Category: Genetics & Stem Cells Authors: E. G ómez, S. Carrocera, D. Martin, P. Herrero, N. Canela, M. Muñoz Source Type: research
Effect of season on reproductive behaviors and fertilization success in cavies (Cavia aperea)
Finding the optimal timing for breeding is crucial for small mammals to ensure survival and maximize lifetime reproductive success. Species living in temperate regions therefore often restrict breeding to seasons with favorable food and weather conditions. Although caviomorph rodents such as guinea pigs are described as non-seasonal breeders, a series of recent publications has shown seasonal adaptations in litter size, offspring birth mass and maternal investment. Here, we aim to test if seasonal patterns of litter size variation found in earlier studies, are mediated by seasonal differences in female estrus length, ferti...
Source: Theriogenology - April 4, 2018 Category: Genetics & Stem Cells Authors: Romy Hribal, Kathrin R übensam, Sandra Bernhardt, Katarina Jewgenow, Anja Guenther Source Type: research
Synergistic effects of resveratrol and melatonin on in vitro maturation of porcine oocytes and subsequent embryo development
Resveratrol and melatonin are known for their antioxidant properties and have various biological activities. The fact that they exhibit possible synergistic effects in phytomedicine researches suggests the use of a combination of these agents to promote porcine in vitro maturation (IVM) of oocytes. Therefore, we investigated the effects of resveratrol and/or melatonin on this process; cumulus-oocyte complexes underwent IVM culture with four different conditions (control, resveratrol, melatonin or their combination). (Source: Theriogenology)
Source: Theriogenology - April 4, 2018 Category: Genetics & Stem Cells Authors: Sanghoon Lee, Jun-Xue Jin, Anukul Taweechaipaisankul, Geon A. Kim, Byeong Chun Lee Source Type: research
Synchronization treatments previous to natural breeding anticipate and improve the pregnancy rate of postpartum primiparous beef cows
Timed AI has become a potential tool to bypass postpartum acyclicity, yet only a small percentage of the world bovine herd is inseminated. Most females are still subjected to bull mating; therefore, the frequent occurrence of postpartum anestrus may compromise their reproductive efficiency. Thus, the aim of this study was to develop an approach that allows the early conception of postpartum primiparous beef cows that are exposed to natural breeding (NB). For this purpose, 350 primiparous Nelore cows 35 –65 d postpartum were allocated into three groups: Control (no hormonal treatment), TNB (hormonal protocol for timed-NB...
Source: Theriogenology - April 4, 2018 Category: Genetics & Stem Cells Authors: R.M. Ferreira, T.L. Conti, R.L. Gon çalves, L.A. Souto, J.N.S. Sales, M.F. Sá Filho, F.M. Elliff, P.S. Baruselli Source Type: research
Differential release of cell-signaling metabolites by male and female bovine embryos cultured in vitro
Male and female early bovine embryos show dimorphic transcription that impacts metabolism. Individual release of metabolites was examined in a 24h single culture medium from Day-6 male and female morulae that developed to Day-7 expanded blastocysts. Embryos were produced in vitro, fertilized with a single bull and cultured in SOFaaci+6 g/L BSA. The embryonic sex was identified (amelogenin gene amplification). Embryos (N = 10 males and N = 10 females) and N = 6 blank samples (i.e. SOFaaci+6 g/L BSA incubated with no embryos) were collected from 3 replicates. (Source: Theriogenology)
Source: Theriogenology - April 4, 2018 Category: Genetics & Stem Cells Authors: E. G ómez, S. Carrocera, D. Martin, P. Herrero, N. Canela, M. Muñoz Source Type: research
Effect of season on reproductive behaviors and fertilization success in cavies (Cavia aperea)
Finding the optimal timing for breeding is crucial for small mammals to ensure survival and maximize lifetime reproductive success. Species living in temperate regions therefore often restrict breeding to seasons with favorable food and weather conditions. Although caviomorph rodents such as guinea pigs are described as non-seasonal breeders, a series of recent publications, has shown seasonal adaptations in litter size, offspring birth mass and maternal investment. Here, we aim to test if seasonal patterns of litter size variation found in earlier studies, are mediated by seasonal differences in female estrus length, fert...
Source: Theriogenology - April 4, 2018 Category: Genetics & Stem Cells Authors: Romy Hribal, Kathrin R übensam, Sandra Bernhardt, Katarina Jewgenow, Anja Guenther Source Type: research
Synergistic effects of resveratrol and melatonin on in vitro maturation of porcine oocytes and subsequent embryo development
Resveratrol and melatonin are known for their antioxidant properties and have various biological activities. The fact that they exhibit possible synergistic effects in phytomedicine researches suggests the use of a combination of these agents to promote porcine in vitro maturation (IVM) of oocytes. Therefore, we investigated the effects of resveratrol and/or melatonin on this process; cumulus-oocyte complexes underwent IVM culture with four different conditions (control, resveratrol, melatonin or their combination). (Source: Theriogenology)
Source: Theriogenology - April 4, 2018 Category: Genetics & Stem Cells Authors: Sanghoon Lee, Jun-Xue Jin, Anukul Taweechaipaisankul, Geon A. Kim, Byeong Chun Lee Source Type: research
Protective effect of Chitosan nanoparticles against the inhibitory effect of linoleic acid supplementation on maturation and developmental competence of bovine oocytes
The current study was conducted to investigate the effect of linoleic acid –treated oocytes during in vitro maturation (IVM) on cumulus cell expansion, oocyte nuclear maturation, blastocyst development and DNA damage of cumulus cells and how its effects were alleviated by supplementation of the maturation medium with Chitosan nanoparticles (CSNPs). Inhibitory effects of LA at a concentration (100 μM/ml) or more significantly decreased the percentage of oocytes reaching metaphase II stage, the rate of fully expanded cumulus cells 24 h after IVM and the percentage of blastocyst rate compared with the control (p (S...
Source: Theriogenology - March 31, 2018 Category: Genetics & Stem Cells Authors: B.R. Abdel-Halim Source Type: research
Development of fertility programs to achieve high 21-day pregnancy rates in high-producing dairy cows
Our objective is to overview the research that lead to the development of fertility programs for high-producing lactating dairy cows using only GnRH and Prostaglandin F2 α (PGF2α), such as Ovsynch with a focus on the role of progesterone in fertility. A key factor affecting fertility to timed-AI is the response to each hormonal treatment of the Ovsynch protocol. Although not required for fertility, cows ovulating to the first GnRH treatment of the Ovsynch protocol (G1) have greater P/AI than cows failing to ovulate. (Source: Theriogenology)
Source: Theriogenology - March 31, 2018 Category: Genetics & Stem Cells Authors: P.D. Carvalho, V.G. Santos, J.O. Giordano, M.C. Wiltbank, P.M. Fricke Source Type: research
Protective effect of Chitosan nanoparticles against the inhibitory effect of linoleic acid supplementation on maturation and developmental competence of bovine oocytes
The current study was conducted to investigate effect of linoleic acid –treated oocytes during in vitro maturation (IVM) on cumulus cell expansion, oocyte nuclear maturation, blastocyst development and DNA damage of cumulus cells and how its effects were alleviated by supplementation of the maturation medium with Chitosan nanoparticles (CSNPs). Inhibitory effects of LA at a concentration (100 μM/ml) or more significantly decreased the percentage of oocytes reaching metaphase II stage, the rate of fully expanded cumulus cells 24 h after IVM and the percentage of blastocyst rate compared with the control (p (Source: Theriogenology)
Source: Theriogenology - March 31, 2018 Category: Genetics & Stem Cells Authors: B.R. Abdel-Halim Source Type: research
Development of fertility programs to achieve high 21-day pregnancy rates in high-producing dairy cows
Our objective is to overview the research that lead to the development of fertility programs for high-producing lactating dairy cows using only GnRH and Prostaglandin F2 α (PGF2α), such as Ovsynch with a focus on the role of progesterone in fertility. A key factor affecting fertility to timed-AI is the response to each hormonal treatment of the Ovsynch protocol. Although not required for fertility, cows ovulating to the first GnRH treatment of the Ovsynch protocol (G1) have greater P/AI than cows failing to ovulate. (Source: Theriogenology)
Source: Theriogenology - March 31, 2018 Category: Genetics & Stem Cells Authors: P.D. Carvalho, V.G. Santos, J.O. Giordano, M.C. Wiltbank, P.M. Fricke Source Type: research
Effect of tropical thermal stress on peri-implantation immune responses in cows
In this study, we investigated the possible immunological factors which may contribute to pregnancy loss during the peri-implantation period under different seasons of tropical conditions. Eighteen Karan Fries cows, six cows in each season (W: winter; HH: hot-humid; HD: hot-dry) were selected. These cows exhibited heat and were brought for artificial insemination (AI; day 0). (Source: Theriogenology)
Source: Theriogenology - March 28, 2018 Category: Genetics & Stem Cells Authors: Mohanned Naif Alhussien, Aarti Kamboj, Mustafa Ahmad Aljader, Bibhudatta S.K. Panda, Munna lal Yadav, Lakshit Sharma, Seid Mohammed, Aasif Ahmad Sheikh, Masoud Lotfan, Rajeev Kapila, A.K. Mohanty, Ajay Kumar Dang Source Type: research
How does the microbial load affect the quality of equine cool-stored semen?
Contaminating bacteria present in stallion ejaculates may compromise sperm quality during storage. Different procedures have been used to reduce the load of microorganisms in semen and avoid bacterial growth during storage. The aims of this study were: 1) to evaluate different techniques to eliminate bacteria in semen 2) to study the relationship between total microflora load (TML) and ROS production; and 3) to determine if TML affects the functionality of cool-stored sperm. Ejaculates from 11 stallions were split and processed in 3 ways: A. (Source: Theriogenology)
Source: Theriogenology - March 26, 2018 Category: Genetics & Stem Cells Authors: E. Varela, J. Rey, E. Plaza, P. Mu ñoz de propios, J.M. Ortiz-Rodríguez, M. Álvarez, L. Anel-López, L. Anel, P. De Paz, M.C. Gil, J.M. Morrell, C. Ortega-Ferrusola Source Type: research
