Stroma cell-derived factor 1 and connexins (37 and 43) are preserved after vitrification and in vitro culture of goat ovarian cortex
This study aimed to evaluate the follicular morphology and development (follicular activation, cell proliferation, and hormone production), as well as the distribution pattern of Connexins 37 and 43 and SDF-1 α after vitrification and in vitro culture of goat ovarian tissue. The study involved four experimental groups: fresh control, vitrified control, fresh culture and vitrified culture. The ovarian fragments were vitrified by a solid surface technique using the Ovarian Tissue Cryosystem and subsequen tly in vitro cultured for 7 days. (Source: Theriogenology)
Source: Theriogenology - May 12, 2018 Category: Genetics & Stem Cells Authors: Nathalie Jiatsa Donfack, Kele Amaral Alves, Benner Geraldo Alves, Rebeca Magalh ães Pedrosa Rocha, Jamily Bezzera Bruno, Marcelo Bertolini, Regiane Rodrigues dos Santos, Sheyla Farhaydes Souza Domingues, José Ricardo De Figueiredo, Johan Smitz, Ana Paul Source Type: research
Stroma cell-derived factor 1 and connexins (37 and 43) are preserved after vitrification and in vitro culture of goat ovarian cortex
This study aimed to evaluate the follicular morphology and development (follicular activation, cell proliferation, and hormone production), as well as the distribution pattern of Connexins 37 and 43 and SDF-1 α after vitrification and in vitro culture of goat ovarian tissue. The study involved four experimental groups: fresh control, vitrified control, fresh culture and vitrified culture. The ovarian fragments were vitrified by a solid surface technique using the Ovarian Tissue Cryosystem and subsequent ly in vitro cultured for 7 days. (Source: Theriogenology)
Source: Theriogenology - May 12, 2018 Category: Genetics & Stem Cells Authors: Nathalie Jiatsa Donfack, Kele Amaral Alves, Benner Geraldo Alves, Rebeca Magalh ães Pedrosa Rocha, Jamily Bezzera Bruno, Marcelo Bertolini, Regiane Santos, Sheyla Farhaydes Souza Domingues, José Ricardo De Figueiredo, Johan Smitz, Ana Paula Ribeiro Rodr Source Type: research
Goat Boule: Isoforms identification, mRNA expression in testis and functional study and promoter methylation profiles
This study was therefore to detect splicing variants of the goat Boule gene and explore their potential roles in meiosis. Three isoforms, denoted as Boule-a, Boule-b, and Boule-c, were identified in the testes of goats using real-time PCR (RT-PCR) and cloning sequencing. (Source: Theriogenology)
Source: Theriogenology - May 12, 2018 Category: Genetics & Stem Cells Authors: Xiaoyan Zhang, Shuai Yu, Qing Yang, Ke Wang, Sihuan Zhang, Chuanying Pan, Hailong Yan, Ruihua Dang, Chuzhao Lei, Hong Chen, Xianyong Lan Source Type: research
Interval of gonadotropin administration for in vitro embryo production from oocytes collected from Holstein calves between 2 and 6 months of age by repeated laparoscopy
In this study, 11 Holstein calves were subjected to up to six LOPU procedures between the ages of 2–6 months at 2–3 weeks i nterval. In all cases, the animals received a CIDR 5 days prior to LOPU and were gonadotropin-stimulated starting at 72 h before LOPU using one of three protocols that were rotated twice among the animals during the study. (Source: Theriogenology)
Source: Theriogenology - May 11, 2018 Category: Genetics & Stem Cells Authors: Hernan Baldassarre, Luke Currin, Laura Michalovic, Anne-Marie Bellefleur, Karina Gutierrez, Rafael G. Mondadori, Werner G. Glanzner, Yasmin Schuermann, Rodrigo C. Bohrer, Naomi Dicks, Rosalba Lopez, Fran çois-Xavier Grand, Christian Vigneault, Patrick Bl Source Type: research
Interval of gonadotropin administration for in vitro embryo production from oocytes collected from Holstein calves between 2 and 6 months of age by repeated laparoscopy
In this study, 11 Holstein calves were subjected to up to six LOPU procedures between the ages of 2-6 months at 2-3 weeks interval. In all cases, the animals received a CIDR 5 days prior to LOPU and were gonadotropin-stimulated starting at 72h before LOPU using one of three protocols that were rotated twice among the animals during the study. (Source: Theriogenology)
Source: Theriogenology - May 11, 2018 Category: Genetics & Stem Cells Authors: Hernan Baldassarre, Luke Currin, Laura Michalovic, Anne-Marie Bellefleur, Karina Gutierrez, Rafael G. Mondadori, Werner G. Glanzner, Yasmin Schuermann, Rodrigo C. Bohrer, Naomi Dicks, Rosalba Lopez, Fran çois-Xavier Grand, Christian Vigneault, Patrick Bl Source Type: research
Conception risk of beef cattle after fixed-time artificial insemination using either SexedUltra ™ 4M sex-sorted semen or conventional semen
The objective of this study was to compare conception rates of female beef cattle inseminated at a fixed-time with either conventional (CON) or SexedUltra ™ sex-sorted (SU) semen. Treatments included CON or SU with two sires represented within each treatment. Cows (n = 316) and heifers (n = 78) from six locations were randomly assigned treatment. Ovulation was synchronized in all females using the industry-standard 7-d CO-Synch + controlled in ternal drug release (CIDR) protocol (100 μg GnRH + CIDR [1.38 g progesterone] on d 0, 25 mg PGF2α at CIDR removal on d 7, and 100 μg GnRH on d 10, 54 h (heifers) or...
Source: Theriogenology - May 10, 2018 Category: Genetics & Stem Cells Authors: B.R. Crites, R. Vishwanath, A.M. Arnett, P.J. Bridges, W.R. Burris, K.R. McLeod, L.H. Anderson Source Type: research
Red wolf (Canis rufus) sperm quality and quantity is affected by semen collection method, extender components, and post-thaw holding temperature
This study's objectives were to further optimize semen extender used for red wolf sperm cryopreservation, investigate effects of post-thaw holding temperature, and to determine if urethral catheterization is an effective method for semen collection in this species. (Source: Theriogenology)
Source: Theriogenology - May 10, 2018 Category: Genetics & Stem Cells Authors: Ashley D. Franklin, William T. Waddell, Karen L. Goodrowe Source Type: research
Protection of GNRH analogue by chitosan-dextran sulfate nanoparticles for intravaginal application in rabbit artificial insemination
The present study was designed to prove new rabbit insemination extenders containing aminopeptidase inhibitors (AMIs) with or without chitosan (CS)-dextran sulfate (DS) nanoparticles entrapping the GnRH analogue. In addition, different hormone concentrations were tested in these extenders, evaluating their in vivo effect on rabbit reproductive performance after artificial insemination. A total of 911 females were inseminated with semen diluted with the four experimental extenders (C4 group: 4 µg buserelin/doe in control medium (Tris-citric acid-glucose supplemented with bestatin 10 µM and EDTA 20 mM), C5 group: 5 µg of ...
Source: Theriogenology - May 10, 2018 Category: Genetics & Stem Cells Authors: L. Casares-Crespo, P. Fern ández-Serrano, M.P. Viudes-de-Castro Source Type: research
Alterations in the distribution of actin and its binding proteins in the porcine endometrium during early pregnancy: Possible role in epithelial remodeling and embryo adhesion
During early pregnancy, uterine epithelial cells undergo major transformations in their cytoskeleton that make the endometrium receptive for conceptus attachment. Actin binding proteins (ABPs) such as cofilin, gelsolin, and vinculin are involved in regulating actin polymerization, severing or crosslinking actin to integrins. However, whether ABPs are involved in epithelial remodeling or embryo adhesion in pigs is unknown. Therefore, the expression and distribution of these proteins were investigated in porcine endometrium on Days 10 and 13 (pre-implantation period), and 16 (attachment phase) of the estrous cycle or pregnan...
Source: Theriogenology - May 8, 2018 Category: Genetics & Stem Cells Authors: Beenu Moza Jalali, Pawel Likszo, Aneta Andronowska, Dariusz J. Skarzynski Source Type: research
Inhibition of cathepsin B activity prevents deterioration in the quality of in vitro aged porcine oocytes
The activity of cathepsin B, a member of the lysosomal protease family, directly correlates with oocyte quality and subsequent embryonic development. However, its biological function during the progression of in vitro aging of oocytes in pigs has not been demonstrated. Here, we showed that cathepsin B activity was dramatically increased during in vitro aged oocytes. The inhibition of cathepsin B activity prevented the decline of the quality of aged oocytes and improved their subsequent developmental comp etence. (Source: Theriogenology)
Source: Theriogenology - May 4, 2018 Category: Genetics & Stem Cells Authors: Shuang Liang, Hao Jiang, Xing-Hui Shen, Jia-Bao Zhang, Nam-Hyung Kim Source Type: research
Evaluation of an alternative treatment protocol by aglepristone to induce parturition in ewes with an experimental model of early pregnancy toxemia
A new protocol with aglepristone to induce parturition in ewes with pregnancy toxemia has been reported in the present manuscript. Four experimental groups were defined: Group AG5 (n=10), Group DEX (n=10), Group NC (n=5) and Group PT (n=5) in which ewes were injected twice with 10 mg/kg of aglepristone and 5 ml dexamethasone in first two groups, respectively; whereas negative control and pregnancy toxemia groups received no treatment for parturition induction. Different clinical parameters associated with parturition in ewes and their newborns were investigated. (Source: Theriogenology)
Source: Theriogenology - May 4, 2018 Category: Genetics & Stem Cells Authors: G.R. Özalp, A. Yavuz, I. Seker, D. Udum-Küçükşen, A. Rişvanlı, Y. Korlu Source Type: research
Reproductive stage and sex steroid hormone levels influence the expression of mesenchymal stromal cell (MSC) markers in the equine endometrium
This study was designed to investigate the changes in endometrial expression of MSC markers during reproductive cycles in mares and the influence of sex steroid hormones on endometrial MSC proliferation in vitro. Endometrial biopsies were collected from pony mares at different reproductive stages (estrus; day 5 and 13 after ovulation; seasonal anestrus; 20 hours and 7days post-partum; n=5 per stage) and were analyzed by RT-qPCR. (Source: Theriogenology)
Source: Theriogenology - May 4, 2018 Category: Genetics & Stem Cells Authors: B. Elisabeth Rink, Juliane Kuhl, Cristina L. Esteves, Hilari M. French, Elaine Watson, Christine Aurich, F. Xavier Donadeu Source Type: research
Inhibition of cathepsin B activity prevents deterioration in the quality of in vitro aged porcine oocytes
The activity of cathepsin B, a member of the lysosomal protease family, directly correlates with oocyte quality and subsequent embryonic development. However, its biological function during the progression of in vitro aging of oocytes in pigs has not been demonstrated. Here, we showed that cathepsin B activity was dramatically increased during in vitro aged oocytes. The inhibition of cathepsin B activity prevented the decline of the quality of aged oocytes and improved their subsequent developmental competence. (Source: Theriogenology)
Source: Theriogenology - May 4, 2018 Category: Genetics & Stem Cells Authors: Shuang Liang, Hao Jiang, Xing-Hui Shen, Jia-Bao Zhang, Nam-Hyung Kim Source Type: research
Exposure to progesterone previous to the protocol of ovulation synchronization increases the follicular diameter and the fertility of suckled Bos indicus cows
The objective was to evaluate the effect of injectable progesterone previous to the timed artificial insemination (TAI) protocol on follicular growth, ovulation and pregnancy rate of suckled Bos indicus cows. In experiment 1, 10 days before the beginning to TAI protocol (D-10), 431 suckled-anestrus Nelore cows (249 multiparous and 182 primiparous), were allocated to one of three treatments groups (control, P4i and P4iGnRH). At this moment, cows in the P4i and P4iGnRH group received 150 mg of injectable progesterone intramuscularly (Sincrogest injet ável®, Ouro Fino, Brazil). (Source: Theriogenology)
Source: Theriogenology - May 2, 2018 Category: Genetics & Stem Cells Authors: L.M.S. Sim ões, R.E. Orlandi, J.P.M. Massoneto, L.A. Scandiuzzi, B.G. Freitas, M.R. Bastos, J.C. Souza, J.N.S. Sales Source Type: research
Conceptus loss in Santa In ês ewes carrying twin pregnancies by natural mating or embryo transfer
Commercial application of reproductive biotechnologies such as multiple ovulation and embryo transfer depends on its overall efficiency. Sheep embryo transfer is gradually gaining wider adoption, but pregnancy rates after embryo transfer remain lower than those derived from natural mating for most breeds. The work was aimed to evaluate embryonic and fetal losses in Santa In ês ewes carrying twin pregnancies by natural mating or embryo transfer. Ewes were subjected to synchronized natural mating by ram effect or used as recipients for embryo transfer. (Source: Theriogenology)
Source: Theriogenology - April 30, 2018 Category: Genetics & Stem Cells Authors: Jos é Carlos Ferreira-Silva, Leopoldo Meyer Freitas Neto, Marcelo Tigre Moura, Fernando Tenório Filho, Luis Rennan Sampaio Oliveira, Claudio Coutinho Bartolomeu, Marcos Antonio Lemos Oliveira Source Type: research
