Testing cryopreserved European eel sperm for hybridization (A. japonica × A. anguilla)
The objective of this study was to assess impact of cryopreserved European eel sperm and Japanese eel native sperm on early fertilization, hatch, survival, and malformation rates of larvae, as well as develop molecular techniques to distinguish different eel species. Eggs from Japanese eel females (Anguilla japonica) were artificially fertilized with sperm of Japanese eel males and cryopreserved sperm from European eel (A. anguilla, extender was modified Tanaka solution and methanol as cryoprotectant). (Source: Theriogenology)
Source: Theriogenology - March 1, 2018 Category: Genetics & Stem Cells Authors: Tam ás Müller, Hajime Matsubara, Yuki Kubara, Ákos Horváth, Balázs Kolics, János Taller, Viktor Stéger, Balázs Kovács, László Horváth, Juan F. Asturiano, David S. Peñaranda, Béla Urbányi Source Type: research
Concentrations of follicle stimulating hormone associated with follicle selection, number of follicles, and ipsilateral vs contralateral relationships in mares
Diameter deviation or follicle selection during a follicular wave begins with continuous growth rate of F1 (dominant or selected follicle; DF) and decreasing growth rate of F2 (largest subordinate). Intraovarian patterns based on presence or absence of the DF and CL are DF −CL, DF, CL, and devoid (neither DF nor CL). The DF and CL relationships in a pair of ovaries are ipsilateral (DF−CL pattern and devoid pattern) and contralateral (DF pattern and CL pattern). The effects of patterns and relationships on F1, F2, and FSH during deviation were determined in 21 mare s. (Source: Theriogenology)
Source: Theriogenology - February 24, 2018 Category: Genetics & Stem Cells Authors: O.J. Ginther, R.R. Domingues, S.V. Dangudubiyyam, E.R. Araujo Source Type: research
Editorial Board
(Source: Theriogenology)
Source: Theriogenology - February 23, 2018 Category: Genetics & Stem Cells Source Type: research
Anti-M üllerian hormone and antral follicle count are more effective for selecting ewes with good potential for in vivo embryo production than the presence of FecGE mutation or eCG pre-selection tests
This study aims to compare four different methods for selecting high responding sheep donors for in vivo embryo production. These methods include a pre-selection eCG test (eCG), antral follicle count (AFC), plasma anti-Müllerian hormone measurement (AMH) and genotyping for the presence of the FecGE mutation (a polymorphism in the GDF9 gene associated with increased ovulation rate). Santa Ines e we lambs (n = 25) underwent superovulation (SOV) with 800 IU equine chorionic gonadotropin (eCG) and the corpus luteum (CL) count was recorded by laparoscopy after eight days. (Source: Theriogenology)
Source: Theriogenology - February 23, 2018 Category: Genetics & Stem Cells Authors: Pedro Henrique Nicolau Pinto, Mario Felipe Alvarez Balaro, Joanna Maria Gon çalves Souza-Fabjan, Lilian dos Santos Ribeiro, Gláucia Mota Bragança, Ceci Ribeiro Leite, Eduardo Kenji Nunes Arashiro, Kleibe de Moraes Silva, Jeferson Ferreira Da Fonseca, F Source Type: research
Anti-M üllerian hormone and antral follicle count are more effective for selecting ewes with good potential for in vivo embryo production than the presence of FecGE mutation or eCG pre-selection tests
This study aims to compare four different methods for selecting high responding sheep donors for in vivo embryo production. These methods include a pre-selection eCG test (eCG), antral follicle count (AFC), plasma anti-M üllerian hormone measurement (AMH) and genotyping for the presence of the FecGE mutation (a polymorphism in the GDF9 gene associated with increased ovulation rate). Santa Ines ewe lambs (n = 25) underwent superovulation (SOV) with 800 IU equine chorionic gonadotropin (eCG) and the corpus luteum (CL) count was recorded by laparoscopy after eight days. (Source: Theriogenology)
Source: Theriogenology - February 23, 2018 Category: Genetics & Stem Cells Authors: Pedro Henrique Nicolau Pinto, Mario Felipe Alvarez Balaro, Joanna Maria Gon çalves Souza-Fabjan, Lilian dos Santos Ribeiro, Gláucia Mota Bragança, Ceci Ribeiro Leite, Eduardo Kenji Nunes Arashiro, Kleibe de Moraes Silva, Jeferson Ferreira Da Fonseca, F Source Type: research
Effects of nutrient restriction and arginine treatment on oxidative stress in the ovarian tissue of ewes during the luteal phase
The aim of this study was to determine whether nutrient restriction and arginine treatment affect energy metabolism changes and oxidative stress through the mitochondrial pathway in the ovarian tissue of ewes during the luteal phase. On days 6 –15 of the estrous cycle, 24 multiparous Hu sheep (BW = 43.56 ± 1.53 kg) were randomly assigned to three groups: control group (CG; n = 6), restriction group (RG; n = 9), and l-arginine group (AG; n = 9) administered Arg treatment (or vehicle) three times per day. (Source: Theriogenology)
Source: Theriogenology - February 22, 2018 Category: Genetics & Stem Cells Authors: Yi-Xuan Guo, Hai-Tao Nie, Chen-Jie Xu, Guo-Min Zhang, Ling-Wei Sun, Ting-Ting Zhang, Zhen Wang, Xu Feng, Pei-Hua You, Feng Wang Source Type: research
Glial cell line-derived neurotrophic factor supplementation promotes bovine in vitro oocyte maturation and early embryo development
Paracrine factors such as glial cell line-derived neurotrophic factor (GDNF), which was originally derived from the supernatants of a rat glioma cell line, play pivotal roles in oocyte maturation and early embryo development in mammals, such as mice, rats, pigs, sheep, and even humans. However, whether GDNF facilitates in vitro oocyte maturation or early embryo development in bovines is not yet known. We show for the first time that GDNF and its receptor, GDNF family receptor alpha-1 (GFRA1), are presented in ovarian follicles at different stages as well as during oocyte maturation and early embryo development. (Source: Theriogenology)
Source: Theriogenology - February 19, 2018 Category: Genetics & Stem Cells Authors: Dong-Hui Wang, Hong-Xia Zhou, Shu-Jun Liu, Cheng-Jie Zhou, Xiang-Wei Kong, Zhe Han, Cheng-Guang Liang Source Type: research
Glial cell line-derived neurotrophic factor supplementation promotes bovine in vitro oocyte maturation and early embryo development
Paracrine factors such as glial cell line-derived neurotrophic factor (GDNF), which was originally derived from the supernatants of a rat glioma cell line, play pivotal roles in oocyte maturation and early embryo development in mammals, such as mice, rats, pigs, sheep, and even humans. However, whether GDNF facilitates in vitro oocyte maturation or early embryo development in bovines is not yet known. We show for the first time that GDNF and its receptor, GDNF family receptor alpha-1 (GFRA1), are presented in ovarian follicles at different stages as well as during oocyte maturation and early embryo development. (Source: Theriogenology)
Source: Theriogenology - February 19, 2018 Category: Genetics & Stem Cells Authors: Dong-Hui Wang, Hong-Xia Zhou, Shu-Jun Liu, Cheng-Jie Zhou, Xiang-Wei Kong, Zhe Han, Cheng-Guang Liang Source Type: research
Editorial Board
(Source: Theriogenology)
Source: Theriogenology - February 17, 2018 Category: Genetics & Stem Cells Source Type: research
Exogenous ascorbic acid enhances vitrification survival of porcine in vitro-developed blastocysts but fails to improve the in vitro embryo production outcomes
In this study, the effects of addition of the antioxidant ascorbic acid (AsA) were evaluated during porcine in vitro embryo production (IVP) and vitrification. In experiment 1, the effects of AsA supplementation during IVM, IVF and IVC were evaluated, using a total of 2744 oocytes in six replicates. The IVM, IVF and embryo IVC media were supplemented or not (control) with 50 μg/mL AsA in all possible com binations. No significant effects of AsA were detected in any of the maturation, fertilization or embryo development parameters assessed. (Source: Theriogenology)
Source: Theriogenology - February 17, 2018 Category: Genetics & Stem Cells Authors: A. Nohalez, C.A. Martinez, I. Parrilla, J. Roca, M.A. Gil, H. Rodriguez-Martinez, E.A. Martinez, C. Cuello Source Type: research
