Placental development during early pregnancy in sheep: Progesterone and estrogen receptor protein expression
The aim of this study was to evaluate the pattern of protein expression of the steroid receptor isoforms of nuclear progesterone receptors (PGR) A and B, and estrogen receptors (ESR1 and 2) in utero-placental compartments during early pregnancy. Utero-placental tissues were collected from days 14 –30 (n = 4 ewes/day), and uterine tissues were collected from non-pregnant ewes on day 10 after estrus (n = 4). Cross sections of formalin-fixed and paraffin embedded tissues were immunofluorescently stained to detect PGRAB, PGRB, ESR1 and ESR2, followed by image generation of entire cross -sections of uterine and utero-...
Source: Theriogenology - April 7, 2018 Category: Genetics & Stem Cells Authors: Soumi Bairagi, Anna T. Grazul-Bilska, Pawel P. Borowicz, Arshi Reyaz, Veselina Valkov, Lawrence P. Reynolds Source Type: research
Fatty acid profile of blood plasma and oviduct and uterine fluid during early and late luteal phase in the horse
During early pregnancy, the secretome of both oviduct and uterus serves as exchange medium for signaling factors between embryo and mother and provides the embryo with nutrients. The preimplantation embryo can utilize the fatty acids (FA) therein via direct incorporation into cell membrane lipid bilayers and for energy production via β-oxidation. The FA concentration and composition of the oviduct (OF) and uterine fluid (UF) might be regulated by ovarian hormones to meet the changing needs of the growing embryo. (Source: Theriogenology)
Source: Theriogenology - April 7, 2018 Category: Genetics & Stem Cells Authors: B. Drews, V. Milojevic, K. Giller, S.E. Ulbrich Source Type: research
Tubulin posttranslational modifications in in vitro matured prepubertal and adult ovine oocytes
Microtubules (MTs), polymers of alpha/beta-tubulin heterodimers, are involved in crucial functions in eukaryotic cells. MTs physiology can be influenced by a variety of post-translational modifications (PTMs), including tyrosination, detyrosination, delta 2 modification, acetylation, polyglutamylation, polyglycylation. In mammalian oocytes, MTs are essential for meiosis, regulating the formation of meiotic spindle and chromosomes movements. Considering that the patterns of tubulin PTMs (tyrosination, detyrosination, acetylation, polyglutamylation and delta 2 modification) have not been investigated in ovine oocytes, this s...
Source: Theriogenology - April 7, 2018 Category: Genetics & Stem Cells Authors: E. Serra, S. Succu, F. Berlinguer, C. Porcu, G.G. Leoni, S. Naitana, S.D. Gadau Source Type: research
Placental development during early pregnancy in sheep: Progesterone and estrogen receptor protein expression
The aim of this study was to evaluate the pattern of protein expression of the steroid receptor isoforms of nuclear progesterone receptors (PGR) A and B, and estrogen receptors (ESR1 and 2) in utero-placental compartments during early pregnancy. Utero-placental tissues were collected from days 14 –30 (n = 4 ewes/day), and uterine tissues were collected from non-pregnant ewes on day 10 after estrus (n = 4). Cross sections of formalin-fixed and paraffin embedded tissues were immunofluorescently stained to detect PGRAB, PGRB, ESR1 and ESR2, followed by image generation of entire cross -sections of uterine and utero-...
Source: Theriogenology - April 7, 2018 Category: Genetics & Stem Cells Authors: Soumi Bairagi, Anna T. Grazul-Bilska, Pawel P. Borowicz, Arshi Reyaz, Veselina Valkov, Lawrence P. Reynolds Source Type: research
Fatty acid profile of blood plasma and oviduct and uterine fluid during early and late luteal phase in the horse
During early pregnancy, the secretome of both oviduct and uterus serves as exchange medium for signaling factors between embryo and mother and provides the embryo with nutrients. The preimplantation embryo can utilize the fatty acids (FA) therein via direct incorporation into cell membrane lipid bilayers and for energy production via β-oxidation. The FA concentration and composition of the oviduct (OF) and uterine fluid (UF) might be regulated by ovarian hormones to meet the changing needs of the growing embryo. (Source: Theriogenology)
Source: Theriogenology - April 7, 2018 Category: Genetics & Stem Cells Authors: B. Drews, V. Milojevic, K. Giller, S.E. Ulbrich Source Type: research
Tubulin posttranslational modifications in in vitro matured prepubertal and adult ovine oocytes
Microtubules (MTs), polymers of alpha/beta-tubulin heterodimers, are involved in crucial functions in eukaryotic cells. MTs physiology can be influenced by a variety of post-translational modifications (PTMs), including tyrosination, detyrosination, delta 2 modification, acetylation, polyglutamylation, polyglycylation. In mammalian oocytes, MTs are essential for meiosis, regulating the formation of meiotic spindle and chromosomes movements. Considering that the patterns of tubulin PTMs (tyrosination, detyrosination, acetylation, polyglutamylation and delta 2 modification) have not been investigated in ovine oocytes, this s...
Source: Theriogenology - April 7, 2018 Category: Genetics & Stem Cells Authors: E. Serra, S. Succu, F. Berlinguer, C. Porcu, G.G. Leoni, S. Naitana, S.D. Gadau Source Type: research
Anti-apoptotic effects of minocycline on ram epididymal spermatozoa exposed to oxidative stress
Cryopreservation causes major damage to sperm cells and thereby, increased apoptosis has been documented as physiologically programmed cells death. The goal of current study was to evaluate the anti-apoptotic effects of minocycline compared to sericin added to diluents on frozen-thawed spermatozoa. Epididymal spermatozoa isolated from 50 pairs testes with motility>80% and total morphological abnormalities (Source: Theriogenology)
Source: Theriogenology - April 6, 2018 Category: Genetics & Stem Cells Authors: Zahra Merati, Abbas Farshad, Amjad Farzinpour, Jalal Rostamzadeh, Mohsen Sharafi Source Type: research
Positive effect of resveratrol against preantral follicles degeneration after ovarian tissue vitrification
This study aimed to evaluate whether the addition of resveratrol to vitrification/thawing medium improves the cryotolerance of preantral follicles enclosed in bovine ovarian fragments. Ovarian fragments were obtained from bovine fetuses and distributed to the following groups: fresh ovarian fragments (control), vitrified (VIT), and vitrified with resveratrol (VIT + RESV). Overall, the mean percentage of normal follicles was greater (P (Source: Theriogenology)
Source: Theriogenology - April 6, 2018 Category: Genetics & Stem Cells Authors: Carina Diniz Rocha, Mayara Mafra Soares, Deize de C ássia Antonino, Jairo Melo Júnior, Renata Ferreira Freitas Mohallem, Ana Paula Ribeiro Rodrigues, José Ricardo Figueiredo, Marcelo Emílio Beletti, José Octavio Jacomini, Benner Geraldo Alves, Kele A Source Type: research
Heat-shock-induced cathepsin B activity during IVF and culture compromises the developmental competence of bovine embryos
Heat stress can cause significant reproductive dysfunction in mammals and previous studies report that expression and activity of cathepsin B (CTSB), a lysosomal cysteine protease, is negatively correlated with the developmental competence of bovine oocytes and embryos. However, the relationship between heat shock (HS) and CTSB remains largely unknown. Here, we investigated the effects of HS during IVF and early embryonic stages of IVC on CTSB activity and developmental competence in bovine embryos. (Source: Theriogenology)
Source: Theriogenology - April 6, 2018 Category: Genetics & Stem Cells Authors: Ken-ichi Yamanaka, Hafiza Khatun, Junki Egashira, Ahmed Zaky Balboula, Hideki Tatemoto, Miki Sakatani, Naoki Takenouchi, Yasuhiko Wada, Masashi Takahashi Source Type: research
Anti-apoptotic effects of minocycline on ram epididymal spermatozoa exposed to oxidative stress
Cryopreservation causes major damage to sperm cells and thereby, increased apoptosis has been documented as physiologically programmed cells death. The goal of current study was to evaluate the anti-apoptotic effects of minocycline compared to sericin added to diluents on frozen-thawed spermatozoa. Epididymal spermatozoa isolated from 50 pairs testes with motility>80% and total morphological abnormalities (Source: Theriogenology)
Source: Theriogenology - April 6, 2018 Category: Genetics & Stem Cells Authors: Zahra Merati, Abbas Farshad, Amjad Farzinpour, Jalal Rostamzadeh, Mohsen Sharafi Source Type: research
Positive effect of resveratrol against preantral follicles degeneration after ovarian tissue vitrification
This study aimed to evaluate whether the addition of resveratrol to vitrification/thawing medium improves the cryotolerance of preantral follicles enclosed in bovine ovarian fragments. Ovarian fragments were obtained from bovine fetuses and distributed to the following groups: fresh ovarian fragments (control), vitrified (VIT), and vitrified with resveratrol (VIT + RESV). Overall, the mean percentage of normal follicles was greater (P (Source: Theriogenology)
Source: Theriogenology - April 6, 2018 Category: Genetics & Stem Cells Authors: Carina Diniz Rocha, Mayara Mafra Soares, Deize de C ássia Antonino, Jairo Melo Júnior, Renata Ferreira Freitas Mohallem, Ana Paula Ribeiro Rodrigues, José Ricardo Figueiredo, Marcelo Emílio Beletti, José Octavio Jacomini, Benner Geraldo Alves, Kele A Source Type: research
Heat-shock-induced cathepsin B activity during IVF and culture compromises the developmental competence of bovine embryos
Heat stress can cause significant reproductive dysfunction in mammals and previous studies report that expression and activity of cathepsin B (CTSB), a lysosomal cysteine protease, is negatively correlated with the developmental competence of bovine oocytes and embryos. However, the relationship between heat shock (HS) and CTSB remains largely unknown. Here, we investigated the effects of HS during IVF and early embryonic stages of IVC on CTSB activity and developmental competence in bovine embryos. (Source: Theriogenology)
Source: Theriogenology - April 6, 2018 Category: Genetics & Stem Cells Authors: Ken-ichi Yamanaka, Hafiza Khatun, Junki Egashira, Ahmed Zaky Balboula, Hideki Tatemoto, Miki Sakatani, Naoki Takenouchi, Yasuhiko Wada, Masashi Takahashi Source Type: research
Presence and function of kisspeptin/KISS1R system in swine ovarian follicles
Kisspeptin and its receptor KISS1R are involved in the neuroendocrine regulation of mammalian reproduction and their role on follicular development and function can be hypothesized. The present work was designed to confirm the immunopresence of kisspeptin and its receptor in the ovary of swine and to study the effects of kisspeptin 10 and its antagonist, kisspeptin 234, on main functional parameters of granulosa cells (i.e. cell proliferation, steroid production, and redox status) as well as their modulatory action on angiogenesis. (Source: Theriogenology)
Source: Theriogenology - April 5, 2018 Category: Genetics & Stem Cells Authors: G. Basini, F. Grasselli, S. Bussolati, R. Ciccimarra, M. Maranesi, A. Bufalari, F. Parillo, M. Zerani Source Type: research
