Tubulin posttranslational modifications in in  vitro matured prepubertal and adult ovine oocytes

Microtubules (MTs), polymers of alpha/beta-tubulin heterodimers, are involved in crucial functions in eukaryotic cells. MTs physiology can be influenced by a variety of post-translational modifications (PTMs), including tyrosination, detyrosination, delta 2 modification, acetylation, polyglutamylation, polyglycylation. In mammalian oocytes, MTs are essential for meiosis, regulating the formation of meiotic spindle and chromosomes movements. Considering that the patterns of tubulin PTMs (tyrosination, detyrosination, acetylation, polyglutamylation and delta 2 modification) have not been investigated in ovine oocytes, this study has been designed to investigate their presence and quantification in in  vitro matured (IVM) adult and prepubertal ovine oocytes.

Source: Theriogenology - April 7, 2018 Category: Genetics & Stem Cells Authors: E. Serra, S. Succu, F. Berlinguer, C. Porcu, G.G. Leoni, S. Naitana, S.D. Gadau Source Type: research