Determination of reference intervals for umbilical cord arterial and venous blood gas analysis of healthy Thoroughbred foals
Although umbilical cord blood gas analysis is considered the best way to assess in utero oxygenation in human neonates, there is limited evaluation of this method in equine neonatology. Our objectives were to assess the practicality of obtaining umbilical cord blood gas samples in the field and to determine umbilical cord arterial and venous blood gas reference intervals (RI) for healthy, newborn foals. Thoroughbred foals>320 days gestation from healthy mares with uneventful pregnancies at one stud farm were evaluated. (Source: Theriogenology)
Source: Theriogenology - May 25, 2018 Category: Genetics & Stem Cells Authors: Sunita S. Jeawon, Lisa M. Katz, Noreen P. Galvin, Ursula M. Fogarty, Vivienne E. Duggan Source Type: research
Inhibition of PDE3A sustains meiotic arrest and gap junction of bovine growing oocytes in in vitro growth culture
In this study, we cultured growing oocytes for 5 days to examine the effects of different phosphodiesterase (PDE) inhibitors on meiotic arrest and acquisition of full maturation competence of growing oocytes, and their gap junctional communication with cumulus cells. (Source: Theriogenology)
Source: Theriogenology - May 25, 2018 Category: Genetics & Stem Cells Authors: Md Hasanur Alam, Jibak Lee, Takashi Miyano Source Type: research
Oxidative stress differentially impacts male and female bovine embryos depending on the culture medium and the stress condition
Male and female embryos are known to differ for their metabolism and response to environmental factors very early in development. The present study aimed to evaluate the response to oxidative stress of male and female bovine embryos at the morula-blastocyst stages in terms of developmental rates, total cell number and apoptotic rates in two culture conditions. Embryos where cultured in a medium supplemented with either 5% fetal calf serum (FCS) or 4 mg/mL bovine serum albumin and a mixture of insulin, transferrin and selenium (BSA-ITS). (Source: Theriogenology)
Source: Theriogenology - May 24, 2018 Category: Genetics & Stem Cells Authors: Matthew Dallemagne, Emmanuelle Ghys, Catalina De Schrevel, Ariane Mwema, Delphine De Troy, Catherine Rasse, Isabelle Donnay Source Type: research
Effects of seminal plasma and flash-freezing on DNA structure of stallion epididymal sperm exposed to different potentiators of DNA damage
In this study, fresh (FS) or flash-frozen (FZ) stallion epididymal sperm were exposed (SP+) or unexposed (SP −) to seminal plasma. Sperm were then evaluated to monitor the degree of change in DNA structure following challenge with chemical (dithiothreitol-DTT), oxidative (iron sulfate; FeSO4) or enzymatic (DNase I) potentiators of DNA damage. For sperm not treated with potentiators (controls), there was no effect of SP treatment (SP− vs. (Source: Theriogenology)
Source: Theriogenology - May 23, 2018 Category: Genetics & Stem Cells Authors: R. Serafini, D.D. Varner, T.L. Blanchard, S.R. Teague, K. LaCaze, C.C. Love Source Type: research
Improved embryo development using high cysteamine concentration during IVM and sperm co-culture with COCs previous to ICSI in bovine
In contrast to other species, intracytoplasmic sperm injection (ICSI) in bovine remains inefficient, resulting in low embryo developmental rates. It is unclear whether such inefficiency is due to the poor response of bovine ooplasms to the injection stimulus, or to the inability of bull sperm to induce oocyte activation. In order to facilitate these events, two strategies were assessed: the use of high concentration of cysteamine [Cys] during IVM; and the selection of sperm attached to cumulus cells after incubation with COCs for ICSI. (Source: Theriogenology)
Source: Theriogenology - May 19, 2018 Category: Genetics & Stem Cells Authors: Natalia Gabriela Canel, Mariana Suv á, Romina Jimena Bevacqua, María Elena Arias, Ricardo Felmer, Daniel Felipe Salamone Source Type: research
Assessment of uterine luminal pH in mares and the effect of dilute vinegar lavage on uterine luminal pH and endometrial health
The objectives of this study were to assess uterine luminal pH in mares during the estrous cycle, and evaluate the effect of dilute vinegar lavage on both uterine luminal pH and endometrial health. The study was conducted using a randomized block design in which eight mares (four Thoroughbred and four Standardbred) were aged matched then randomly assigned to two groups. (Source: Theriogenology)
Source: Theriogenology - May 18, 2018 Category: Genetics & Stem Cells Authors: Renee L. Thompson, Allan J. Gunn, Cyril P. Stephen, Heather Ip, Victoria J. Brookes Source Type: research
Combined effects of resveratrol and epigallocatechin-3-gallate on post thaw boar sperm and IVF parameters
Frozen-thawed boar semen suffer a fertility decrease that negatively affects its widespread use. In recent years supplementing frozen-thawed boar sperm with different antioxidants gave interesting and promising results; the aim of the present work was to study the effect of supplementing boar sperm thawing medium for 1 h with combination of epigallocatechin-3-gallate (EGCG, 50 μM) and Resveratrol (R, 2 mM), on boar sperm motility (assessed by CASA), viability, acrosome integrity, mitochondrial function, lipid peroxidation and DNA integrity (assessed by flow cytometry), protein tyrosine phosphorylation (ass essed by...
Source: Theriogenology - May 18, 2018 Category: Genetics & Stem Cells Authors: Diego Bucci, Marcella Spinaci, Marc Yeste, Beatrice Mislei, Beatrice Gadani, Noelia Prieto Martinez, Charles Love, Gaetano Mari, Carlo Tamanini, Giovanna Galeati Source Type: research
The expression of kisspeptin and its receptor in the domestic cat ovary and uterus in different stages of the ovarian cycle
In this study, we aimed: (1) to investigate the localization pattern of kisspeptin and its receptor in the domestic cat ovary and uterus throughout the ovarian cycle using immunohistochemistry; and (2) to compare the relative expression of ovarian Kiss1 mRNA levels at different ovarian stages with qPCR analysis. (Source: Theriogenology)
Source: Theriogenology - May 18, 2018 Category: Genetics & Stem Cells Authors: P. Tanyapanyachon, O. Amelkina, K. Chatdarong Source Type: research
Effect of urine contamination on stallion semen freezing ability
Urospermia is a common ejaculatory dysfunction of stallions. Current practice suggests that urine contaminated semen should not be used for cryopreservation. The aim of this study was to determine effects of urine contamination on semen cryopreservation. Sixty-five ejaculates from eight stallions were divided into no urine (CONT), low (20% urine, LOW), and high (50% urine, HIGH) samples. Semen was extended with a commercial cooling extender, cushion-centrifuged, resuspended to 200 million/mL in a commercial egg-yolk based extender, and cryopreserved. (Source: Theriogenology)
Source: Theriogenology - May 17, 2018 Category: Genetics & Stem Cells Authors: R.E. Ellerbrock, J. Honorato, B.R. Curcio, J.L. Stewart, J.A.T. Souza, C.C. Love, F.S. Lima, I.F. Canisso Source Type: research
Variables affecting semen quality and its relation to fertility in the dog: A retrospective study.
This study evaluated the effects of dogs ’ age (young: 0-24 months, adult: 25-84 months, old:>84 months), size (small:40 kg) and reason for semen collection (evaluation, artificial insemination, cryopreservation or research purposes) on the seminal characteristics and related the main seminal characteristics with fresh AI results. (Source: Theriogenology)
Source: Theriogenology - May 17, 2018 Category: Genetics & Stem Cells Authors: Matteo Tesi, Chiara Sabatini, Iacopo Vannozzi, Gloria Di Petta, Duccio Panzani, Francesco Camillo, Alessandra Rota Source Type: research
Anti-M üllerian hormone as a biomarker for acute testicular degeneration caused by toxic insults to stallion testes
Recently, anti-M üllerian hormone (AMH) was validated as a reliable marker of testicular damage caused by various chemotherapy drugs in humans and in mice. In horses, the reference values of AMH concentrations in normal stallions, during different seasons of a year, have been recently reported. However, this hormon e was not evaluated in subfertile or infertile stallions with testicular damage. Therefore, the objective of this study was to investigate the effects of experimentally induced testicular degeneration on the concentration of AMH in stallions. (Source: Theriogenology)
Source: Theriogenology - May 17, 2018 Category: Genetics & Stem Cells Authors: Malgorzata Pozor, Alan J. Conley, Janet F. Roser, Margaret Nolin, Gina Zambrano, Scott P. Runyon, Audrey A. Kelleman, Margo L. Macpherson Source Type: research
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(Source: Theriogenology)
Source: Theriogenology - May 16, 2018 Category: Genetics & Stem Cells Source Type: research
Progesterone stimulates the long-distance migration of capacitated ram spermatozoa through viscous media under geotactic condition
Forward progressive motility of spermatozoa is an essential prerequisite for reproductive success, and sperm navigation is assisted by guidance mechanisms that may depend on micro-environmental factors. In the present study, we performed an integrated analysis of long-distance ram sperm migration in vitro that combined two environmental factors (10 μM progesterone and a geotactic effect) and the physiological status of the cells (capacitation treatment). A penetration assay was used in which spermatozoa had to travel 20 mm in a viscous medium (two media of differing viscosity: acrylamide and hyaluronic acid) through a tub...
Source: Theriogenology - May 15, 2018 Category: Genetics & Stem Cells Authors: Carmen Mart ínez-Rodríguez, Luis Anel-López, Mercedes Alvarez, Cristina Ortega-Ferrusola, Juan Carlos Boixo, Fernando J. Peña, Luis Anel, Paulino de Paz Source Type: research
Anti-M üllerian Hormone profiling in prepubertal horses and its relationship with gonadal function
The objectives of the present study were to (1) assess AMH, LH, FSH, progesterone (females) and testosterone (males) dynamics in prepubertal horses from birth onwards and (2) determine whet her AMH concentrations detected in plasma of prepubertal female and male horses are correlated with postpubertal gonadal development. (Source: Theriogenology)
Source: Theriogenology - May 15, 2018 Category: Genetics & Stem Cells Authors: Dragos Scarlet, Manuela Wulf, Juliane Kuhl, Martin K öhne, Natascha Ille, Alan Conley, Christine Aurich Source Type: research
Endocrine and metabolic profile of peripubertal Standardbred colts
The objectives of this study were to determine the concentrations of reproductive and metabolic hormones during the peripubertal period and to assess their relationship with testicular development and body fat deposition. Blood samples were collected from 23 healthy Standardbred colts every four weeks for twelve months. Colts were weighed monthly, and percent of body fat and testicular volume estimated by ultrasound. Onset of puberty was determined as the month when testosterone was two standard deviations above the previous mean. (Source: Theriogenology)
Source: Theriogenology - May 14, 2018 Category: Genetics & Stem Cells Authors: Robyn E. Ellerbrock, Igor F. Canisso, Fabio S. Lima, Clifford F. Shipley, Donald L. Thompson, Alan J. Conley, Kevin H. Kline Source Type: research
