Effects of seminal plasma and flash-freezing on DNA structure of stallion epididymal sperm exposed to different potentiators of DNA damage

In this study, fresh (FS) or flash-frozen (FZ) stallion epididymal sperm were exposed (SP+) or unexposed (SP −) to seminal plasma. Sperm were then evaluated to monitor the degree of change in DNA structure following challenge with chemical (dithiothreitol-DTT), oxidative (iron sulfate; FeSO4) or enzymatic (DNase I) potentiators of DNA damage. For sperm not treated with potentiators (controls), there was no effect of SP treatment (SP− vs.

Source: Theriogenology - May 23, 2018 Category: Genetics & Stem Cells Authors: R. Serafini, D.D. Varner, T.L. Blanchard, S.R. Teague, K. LaCaze, C.C. Love Source Type: research