Cryopreservation and in vitro culture of white-tailed deer ovarian tissue
The aims of this study were to evaluate (1) the survivability of white-tailed deer ovarian tissue after cryopreservation by slow-freezing (SF) and vitrification (VIT) techniques and in vitro culture (IVC) for up to 7 days, and (2) the effects of cryopreservation techniques on protein expression of proliferative and apoptotic markers of ovarian tissue pre- and post-in vitro culture. Ovaries (n = 14) of seven white-tailed deer fawns ( (Source: Theriogenology)
Source: Theriogenology - March 8, 2018 Category: Genetics & Stem Cells Authors: G.D.A. Gastal, F.L.N. Aguiar, A.P.R. Rodrigues, J.M. Scimeca, G.A. Apgar, W.J. Banz, J.M. Feugang, E.L. Gastal Source Type: research
Cryopreservation and in vitro culture of white-tailed deer ovarian tissue
The aims of this study were to evaluate (1) the survivability of white-tailed deer ovarian tissue after cryopreservation by slow-freezing (SF) and vitrification (VIT) techniques and in vitro culture (IVC) for up to 7 days, and (2) the effects of cryopreservation techniques on protein expression of proliferative and apoptotic markers of ovarian tissue pre- and post-in vitro culture. Ovaries (n = 14) of seven white-tailed deer fawns ( (Source: Theriogenology)
Source: Theriogenology - March 8, 2018 Category: Genetics & Stem Cells Authors: G.D.A. Gastal, F.L.N. Aguiar, A.P.R. Rodrigues, J.M. Scimeca, G.A. Apgar, W.J. Banz, J.M. Feugang, E.L. Gastal Source Type: research
Eventual re-vitrification or storage in liquid nitrogen vapor does not jeopardize the practical handling and transport of vitrified pig embryos
This study aimed (1) to evaluate the in vitro post-warming survival of porcine embryos after re-vitrification and (2) to assess the efficacy of transport of embryos in dry shipper (DS) in maintaining the viability and quality of vitrified embryos for a 3-day period. Embryos at the compacted or cavitating morula (CCM) and unhatched blast ocyst (UBL) stages were surgically obtained from weaned, crossbred sows. In the first experiment, more than 85% of the embryos survived an initial vitrification and warming and achieved comparable survival rates to those of their fresh counterparts. (Source: Theriogenology)
Source: Theriogenology - March 7, 2018 Category: Genetics & Stem Cells Authors: Alicia Nohalez, Cristina A. Martinez, Inmaculada Parrilla, Carolina Maside, Jordi Roca, Mar ía A. Gil, Heriberto Rodriguez-Martinez, Emilio A. Martinez, Cristina Cuello Source Type: research
Macroscopic characteristics of the umbilical cord in Standardbred, Thoroughbred and Warmblood horses
The umbilical cord (UC), the connection between mother and fetus via the umbilical vessels, carries nutrients and oxygenated blood to the fetus through the umbilical vein and removes deoxygenated blood and waste products via the umbilical arteries. It is designed to protect blood flow to the fetus during pregnancy. In equine medicine, only a few studies have described the UC, and most of these involved Thoroughbreds. The present study describes and compares the macroscopic features of the equine umbilical cord in three different breeds and in relation to the foal's gender. (Source: Theriogenology)
Source: Theriogenology - March 7, 2018 Category: Genetics & Stem Cells Authors: Jole Mariella, Eleonora Iacono, Aliai Lanci, Barbara Merlo, Caterina Palermo, Lee Morris, Carolina Castagnetti Source Type: research
Eventual re-vitrification or storage in liquid nitrogen vapor does not jeopardize the practical handling and transport of vitrified pig embryos
This study aimed (1) to evaluate the in vitro post-warming survival of porcine embryos after re-vitrification and (2) to assess the efficacy of transport of embryos in dry shipper (DS) in maintaining the viability and quality of vitrified embryos for a 3-day period. Embryos at the compacted or cavitating morula (CCM) and unhatched blastocyst (UBL) stages were surgically obtained from weaned, crossbred sows. In the first experiment, more than 85% of the embryos survived an initial vitrification and warming and achieved comparable survival rates to those of their fresh counterparts. (Source: Theriogenology)
Source: Theriogenology - March 7, 2018 Category: Genetics & Stem Cells Authors: Alicia Nohalez, Cristina A. Martinez, Inmaculada Parrilla, Jordi Roca, Mar ía A. Gil, Heriberto Rodriguez-Martinez, Emilio A. Martinez, Cristina Cuello Source Type: research
Macroscopic characteristics of the umbilical cord in Standardbred, Thoroughbred and Warmblood horses
The umbilical cord (UC), the connection between mother and fetus via the umbilical vessels, carries nutrients and oxygenated blood to the fetus through the umbilical vein and removes deoxygenated blood and waste products via the umbilical arteries. It is designed to protect blood flow to the fetus during pregnancy. In equine medicine, only a few studies have described the UC, and most of these involved Thoroughbreds. The present study describes and compares the macroscopic features of the equine umbilical cord in three different breeds and in relation to the foal's gender. (Source: Theriogenology)
Source: Theriogenology - March 7, 2018 Category: Genetics & Stem Cells Authors: Mariella Jole, Eleonora Iacono, Aliai Lanci, Barbara Merlo, Palermo Caterina, Lee Morris, Castagnetti Carolina Source Type: research
Foreword
In the past 40 years (my life time carrier as a reproductive scientist), reproductive physiology has always been an active research field. Nowadays, this interest may be fading as it is common belief that most of the main reproductive problems can be solved with the existing tools and that training vet students in reproduction is no more a challenge! Despite this belief, there still is a large inflow of scientific manuscripts dedicated to reproduction in scientific journals. Indeed, a recent Scopus search combining the words “reproduction”, “cow”, “sow”, “cattle” or “swine” revealed that, between 2008 a...
Source: Theriogenology - March 4, 2018 Category: Genetics & Stem Cells Authors: Marc-Antoine Driancourt Source Type: research
Editorial Board
(Source: Theriogenology)
Source: Theriogenology - March 4, 2018 Category: Genetics & Stem Cells Source Type: research
Histology confirms that macroscopic evaluation of ovaries is a valid method for the assessment of the reproductive status in wild boar
Research on reproduction and fertility is an essential fundament of management strategies in European wild boar. Concerning the examination of the reproductive tract, methods still lack conformity and comparability. So far the reproductive status of female wild boar has been assessed by ovarian macroscopic examination. In order to find out if this is a valid method, 21 ovaries of 8 –34 months old animals covering (early) follicular phase and cyclic inactivity were evaluated microscopically. Number and dimension of follicles as well as the occurrence of atretic follicles were compared and related to age, weight, ovary wei...
Source: Theriogenology - March 2, 2018 Category: Genetics & Stem Cells Authors: Friederike Geth öffer, Christiane Pfarrer, Ursula Siebert Source Type: research
Lysophosphatidic acid increases in vitro maturation efficiency via uPA-uPAR signaling pathway in cumulus cells
Lysophosphatidic acid (LPA) is a phospholipid-derived signaling molecule with biological activities, such as stimulating cell proliferation, differentiation and migration. In the present study, we examined the effect of LPA on porcine oocytes during in vitro maturation (IVM) and subsequent embryonic development following parthenogenetic activation (PA) and in vitro fertilization (IVF). During IVM, the maturation medium was supplemented with various concentrations of LPA (0, 10, 30, and 60 μM). After 42 h of IVM, the 30 μM LPA-treated gr oup showed a significant (P (Source: Theriogenology)
Source: Theriogenology - March 2, 2018 Category: Genetics & Stem Cells Authors: Seon-Ung Hwang, Kyu-Jun Kim, Eunhye Kim, Junchul David Yoon, Kyu Mi Park, Minghui Jin, Yongquan Han, Mirae Kim, Gabsang Lee, Sang-Hwan Hyun Source Type: research
Histology confirms that macroscopic evaluation of ovaries is a valid method for the assessment of the reproductive status in wild boar
Research on reproduction and fertility is an essential fundament of management strategies in European wild boar. Concerning the examination of the reproductive tract, methods still lack conformity and comparability. So far the reproductive status of female wild boar has been assessed by ovarian macroscopic examination. In order to find out if this is a valid method, 21 ovaries of 8 –34 months old animals covering (early) follicular phase and cyclic inactivity were evaluated microscopically. Number and dimension of follicles as well as the occurrence of atretic follicles were compared and related to age, weight, ovary wei...
Source: Theriogenology - March 2, 2018 Category: Genetics & Stem Cells Authors: Friederike Geth öffer, Christiane Pfarrer, Ursula Siebert Source Type: research
Lysophosphatidic acid increases in vitro maturation efficiency via uPA-uPAR signaling pathway in cumulus cells
Lysophosphatidic acid (LPA) is a phospholipid-derived signaling molecule with biological activities, such as stimulating cell proliferation, differentiation and migration. In the present study, we examined the effect of LPA on porcine oocytes during in vitro maturation (IVM) and subsequent embryonic development following parthenogenetic activation (PA) and in vitro fertilization (IVF). During IVM, the maturation medium was supplemented with various concentrations of LPA (0, 10, 30, and 60 μM). After 42 h of IVM, the 30 μM LPA-treated group showed a significant (P (Source: Theriogenology)
Source: Theriogenology - March 2, 2018 Category: Genetics & Stem Cells Authors: Seon-Ung Hwang, Kyu-Jun Kim, Eunhye Kim, Junchul David Yoon, Kyu Mi Park, Minghui Jin, Yongquan Han, Mirae Kim, Gabsang Lee, Sang-Hwan Hyun Source Type: research
Testing cryopreserved European eel sperm for hybridization (A. japonica × A. anguilla)
The objective of this study was to assess impact of cryopreserved European eel sperm and Japanese eel native sperm on early fertilization, hatch, survival, and malformation rates of larvae, as well as develop molecular techniques to distinguish different eel species. Eggs from Japanese eel females (Anguilla japonica) were artificially fertilized with sperm of Japanese eel males and cryopreserved sperm from European eel (A. anguilla, extender was modified Tanaka solution and methanol as cryoprotectant). (Source: Theriogenology)
Source: Theriogenology - March 1, 2018 Category: Genetics & Stem Cells Authors: Tam ás Müller, Hajime Matsubara, Yuki Kubara, Ákos Horváth, Balázs Kolics, János Taller, Viktor Stéger, Balázs Kovács, László Horváth, Juan F. Asturiano, David S. Peñaranda, Béla Urbányi Source Type: research
