Testing cryopreserved European eel sperm for hybridization (A.  japonica × A. anguilla)

The objective of this study was to assess impact of cryopreserved European eel sperm and Japanese eel native sperm on early fertilization, hatch, survival, and malformation rates of larvae, as well as develop molecular techniques to distinguish different eel species. Eggs from Japanese eel females (Anguilla japonica) were artificially fertilized with sperm of Japanese eel males and cryopreserved sperm from European eel (A.  anguilla, extender was modified Tanaka solution and methanol as cryoprotectant).
Source: Theriogenology - Category: Genetics & Stem Cells Authors: Source Type: research
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