Molecular characterization of voltage-gated potassium channel (Kv) and its importance in functional dynamics in bull spermatozoa
Present study was undertaken to characterize the voltage gated potassium channel (Kv 1.1) in bull spermatozoa using sixty four ejaculates collected from four Hariana bulls. Functional characterization was undertaken using a selective blocker of Kv channel, 4-Aminopyridine (4-AP) while molecular presence of Kv on bull spermatozoa by immunoblotting and indirect immunofluorescence. Three sets of 100 μL diluted sperm samples namely-negative control (100 μL of sperm dilution medium (SDM) containing 10 × 106 cells), vehicle control (99 μL of SDM containing 10 × 106 cells, and DMSO- 1 μL) and 4-AP tr...
Source: Theriogenology - March 26, 2018 Category: Genetics & Stem Cells Authors: Rishi Kumar Gupta, Dilip Kumar Swain, Vijay Singh, Mukul Anand, Soumen Choudhury, Sarvajeet Yadav, Atul Saxena, Satish Kumar Garg Source Type: research
Effects of benign prostatic hyperplasia and finasteride therapy on prostatic blood flow in dogs
Benign prostatic hyperplasia (BPH) is characterized by an enlargement of the prostate accompanied by an increase in prostatic blood perfusion and vascularization. The most indicated treatment is to perform orchiectomy, however, medical treatment with finasteride can be an option for breeding dogs or elderly animals with a critical health status. In dogs, the influence of medical treatment on prostatic hemodynamics is still unknown. Therefore, this study aimed to evaluate the effects of benign prostatic hyperplasia and finasteride therapy on hemodynamic and vascular features of the canine prostate. (Source: Theriogenology)
Source: Theriogenology - March 26, 2018 Category: Genetics & Stem Cells Authors: D.S.R. Angrimani, G.R. Silvestrini, M.M. Brito, R.A. Abreu, L.L. Almeida, C.I. Vannucchi Source Type: research
Improved uterine immune mediators in Holstein cows supplemented with rumen-protected methionine and discovery of neutrophil extracellular traps (NET)
The objective of this study was to observe the effects of rumen-protected methionine (RPM) on plasma amino acid concentrations, uterine cytology, immunohistochemistry (IHC) of glutathione peroxidase 1 (GPX) and superoxide dismutase 1 (SOD), and to confirm neutrophil extracellular trap (NET) formation. Multiparous Holstein cows (n = 20) were randomly assigned to two treatments starting at 21 d before calving until 73 days in milk (DIM). (Source: Theriogenology)
Source: Theriogenology - March 26, 2018 Category: Genetics & Stem Cells Authors: S.L. Stella, D.A. Velasco-Acosta, C. Skenandore, Z. Zhou, A. Steelman, D. Luchini, F.C. Cardoso Source Type: research
Effect of inhibin A on proliferation of porcine granulosa cells in vitro
Inhibins regulate folliculogenesis, gametogenesis and hormone secretion via endocrine, paracrine and autocrine manners, and play roles in encouraging or suppressing proliferation of cells. In order to investigate the effects of inhibin A on proliferation and apoptosis of porcine granulosa cells (GCs), GCs were isolated from ovarian follicles (3 –6 mm), treated with inhibin A at different concentrations. The cell viability, mitochondrial membrane potential (MMP), expression of proliferation-related genes and cell cycle were detected. (Source: Theriogenology)
Source: Theriogenology - March 26, 2018 Category: Genetics & Stem Cells Authors: Wanhong Li, Chunjin Li, Shuxiong Chen, Lina Sun, Hongjiao Li, Lu Chen, Xu Zhou Source Type: research
Effects of maternal dietary selenium (Se-enriched yeast) on testis development, testosterone level and testicular steroidogenesis-related gene expression of their male kids in Taihang Black Goats
To investigate the effects of maternal dietary selenium (Se-enriched yeast) on testis development, testosterone level and steroidogenesis-related gene expression in testis of their male kids, selected pregnant Taihang Black Goats were randomly allotted to four treatment groups. They were fed the basal gestation and lactation diets supplemented with 0 (control), 0.5, 2.0 and 4.0 mg of Se/kg DM. Thirty days after weaning, testes were collected from the kids. After the morphological development status of testis was examined, tissue samples were collected for analyzing testosterone concentration and histological parameters....
Source: Theriogenology - March 26, 2018 Category: Genetics & Stem Cells Authors: Lei Shi, Ruigao Song, Xiaolei Yao, Yunli Duan, Youshe Ren, Chunxiang Zhang, Wenbin Yue, Fulin Lei Source Type: research
How does the microbial load affect the quality of equine cool-stored semen?
Contaminating bacteria present in stallion ejaculates may compromise sperm quality during storage. Different procedures have been used to reduce the load of microorganisms in semen and avoid bacterial growth during storage. The aims of this study were: 1) to evaluate different techniques to eliminate bacteria in semen 2) to study the relationship between total microflora load (TML) and ROS production; and 3) to determine if TML affects the functionality of cool-stored sperm. Ejaculates from 11 stallions were split and processed in 3 ways: A. (Source: Theriogenology)
Source: Theriogenology - March 26, 2018 Category: Genetics & Stem Cells Authors: E. Varela, E. Plaza, P. Mu ñoz de propios, J.M. Ortiz-Rodríguez, M. Álvarez, L. Anel-López, L. Anel, P. De Paz, M.C. Gil, J. Rey, J.M. Morrell, C. Ortega-Ferrusola Source Type: research
Molecular characterization of voltage-gated potassium channel (Kv) and its importance in functional dynamics in bull spermatozoa
Present study was undertaken to characterize the voltage gated potassium channel (Kv 1.1) in bull spermatozoa using sixty four ejaculates collected from four Hariana bulls. Functional characterization was undertaken using a selective blocker of Kv channel, 4-Aminopyridine (4-AP) while molecular presence of Kv on bull spermatozoa by immunoblotting and indirect immunofluorescence. Three sets of 100 μL diluted sperm samples namely-negative control (100 μL of sperm dilution medium (SDM) containing 10 × 106 cells), vehicle control (99 μL of SDM containing 10 × 106 cells, and DMSO- 1 μL) and 4-AP tr...
Source: Theriogenology - March 26, 2018 Category: Genetics & Stem Cells Authors: Rishi K. Gupta, Dilip Kumar Swain, Vijay Singh, Mukul Anand, Soumen Choudhury, Sarvajeet Yadav, Atul Saxena, Satish K. Garg Source Type: research
Effects of benign prostatic hyperplasia and finasteride therapy on prostatic blood flow in dogs
Benign prostatic hyperplasia (BPH) is characterized by an enlargement of the prostate accompanied by an increase in prostatic blood perfusion and vascularization. The most indicated treatment is to perform orchiectomy, however, medical treatment with finasteride can be an option for breeding dogs or elderly animals with a critical health status. In dogs, the influence of medical treatment on prostatic hemodynamics is still unknown. Therefore, this study aimed to evaluate the effects of benign prostatic hyperplasia and finasteride therapy on hemodynamic and vascular features of the canine prostate. (Source: Theriogenology)
Source: Theriogenology - March 26, 2018 Category: Genetics & Stem Cells Authors: D.S.R. Angrimani, G.R. Silvestrini, M.M. Brito, R.A. Abreu, L.L. Almeida, C.I. Vannucchi Source Type: research
Improved uterine immune mediators in Holstein cows supplemented with rumen-protected methionine and discovery of neutrophil extracellular traps (NET)
The objective of this study was to observe the effects of rumen-protected methionine (RPM) on plasma amino acid concentrations, uterine cytology, immunohistochemistry (IHC) of glutathione peroxidase 1 (GPX) and superoxide dismutase 1 (SOD), and to confirm neutrophil extracellular trap (NET) formation. Multiparous Holstein cows (n = 20) were randomly assigned to two treatments starting at 21 d before calving until 73 days in milk (DIM). (Source: Theriogenology)
Source: Theriogenology - March 26, 2018 Category: Genetics & Stem Cells Authors: S.L. Stella, D.A. Velasco-Acosta, C. Skenandore, Z. Zhou, A. Steelman, D. Luchini, F.C. Cardoso Source Type: research
Effect of inhibin A on proliferation of porcine granulosa cells in vitro
Inhibins regulate folliculogenesis, gametogenesis and hormone secretion via endocrine, paracrine and autocrine manners, and play roles in encouraging or suppressing proliferation of cells. In order to investigate the effects of inhibin A on proliferation and apoptosis of porcine granulosa cells (GCs), GCs were isolated from ovarian follicles (3 –6 mm), treated with inhibin A at different concentrations. The cell viability, mitochondrial membrane potential (MMP), expression of proliferation-related genes and cell cycle were detected. (Source: Theriogenology)
Source: Theriogenology - March 26, 2018 Category: Genetics & Stem Cells Authors: Wanhong Li, Chunjin Li, Shuxiong Chen, Lina Sun, Hongjiao Li, Lu Chen, Xu Zhou Source Type: research
Effects of maternal dietary selenium (Se-enriched yeast) on testis development, testosterone level and testicular steroidogenesis-related gene expression of their male kids in Taihang Black Goats
To investigate the effects of maternal dietary selenium (Se-enriched yeast) on testis development, testosterone level and steroidogenesis-related gene expression in testis of their male kids, selected pregnant Taihang Black Goats were randomly allotted to four treatment groups. They were fed the basal gestation and lactation diets supplemented with 0 (control), 0.5, 2.0 and 4.0 mg of Se/kg DM. Thirty days after weaning, testes were collected from the kids. After the morphological development status of testis was examined, tissue samples were collected for analyzing testosterone concentration and histological parameters....
Source: Theriogenology - March 26, 2018 Category: Genetics & Stem Cells Authors: Lei Shi, Ruigao Song, Xiaolei Yao, Yunli Duan, Youshe Ren, Chunxiang Zhang, Wenbin Yue, Fulin Lei Source Type: research
Reference range of gestational uterine artery resistance index in small canine breeds
The objectives of this study were to evaluate the uterine artery blood flow during the second half of normal pregnancy in small breed dogs and to determine the RI reference values of this artery in these bitches. Fifty-two, 1.5 –6.5 kg, purebred healthy pregnant bitches were included in this study. Doppler ultrasonographic examinations of the uterus were performed every 10 days from Day 30 of the estrous cycle to parturition. Peak systolic velocity (PSV) and end diastolic velocity (EDV) of the uterine artery were measur ed while RI [(PSV−EDV)/PSV] was automatically calculated. (Source: Theriogenology)
Source: Theriogenology - March 22, 2018 Category: Genetics & Stem Cells Authors: P.R. Batista, C. Gobello, A. Rube, J.P. Barrena, N.E. Re, P.G. Blanco Source Type: research
The anti-inflammatory effect of exogenous lactoferrin on breeding-induced endometritis when administered post-breeding in susceptible mares
The deposition of semen into the uterus of the horse induces a transient innate immune response that lasts 24 –36 h in the normal mare. There exists a subset of mares that are unable to resolve this inflammation in a timely manner, and are classified as susceptible to the disease of persistent breeding-induced endometritis (PBIE). Lactoferrin is a protein of interest as a potential therapeutic for this persistent inflammation due to its anti-inflammatory and bactericidal properties. The addition of human recombinant lactoferrin (hrLF) to the insemination dose was previously shown to suppress mRNA expression of the pro-...
Source: Theriogenology - March 22, 2018 Category: Genetics & Stem Cells Authors: C.E. Fedorka, K.E. Scoggin, Y.L. Boakari, N.E. Hoppe, E.L. Squires, B.A. Ball, M.H.T. Troedsson Source Type: research
Angiocoupling between the dominant follicle and corpus luteum during waves 1 and 2 in Bos taurus heifers
The local two-way effect of the future and established dominant follicle (DF) and corpus luteum (CL) on color-Doppler signals of blood flow on each other (angiocoupling) was studied from ovulation to 16 days after ovulation during wave 1 and for the first time in wave 2 in Bos taurus heifers (N = 24). Identity of follicles greater than 4 mm was maintained from day to day. Blood-flow signals in the follicle wall and in the CL were estimated after scanning the entire structure. Ovarian patterns were defined based on the presence of a DF and CL: DF−CL pattern (DF and CL in the same o vary), DF pattern or CL pattern (...
Source: Theriogenology - March 22, 2018 Category: Genetics & Stem Cells Authors: R.R. Domingues, O.J. Ginther Source Type: research
Hormonal strategy to reduce suckled beef cow handling for timed artificial insemination with sex-sorted semen
Two experiments were conducted to assess a hormonal strategy developed to reduce animal handling for timed artificial insemination (TAI) with sex-sorted semen. Four-hundred ninety-one (491) suckled beef cows received a progesterone (P4) intravaginal device and 2 mg intramuscular (im) injection of estradiol benzoate (EB) on a randomly chosen day of the estrus cycle (Day 0) in Experiment 1. Cows were treated with 500 μg of sodic cloprostenol (PGF2α) and with 300 IU of eCG at P4 device removal (Day 8); these cows were also randomly assigned to receive 1 mg of estradiol cypionate (EC) administered at P4 device remova...
Source: Theriogenology - March 22, 2018 Category: Genetics & Stem Cells Authors: M.A.V. Silva, C.S. Santos, I.G. Fran ça, H.G. Pereira, M.F. Sá Filho, B.G. Freitas, B.M. Guerreiro, A. Faquim, P.S. Baruselli, J.R.S. Torres-Júnior Source Type: research
