In vitro influence of selenium on the proliferation of and steroidogenesis in goat luteinized granulosa cells
In this study, we investigated the effects of Selenium (Se) on the proliferation of and steroidogenesis in goat luteinized granulosa cells (LGCs) and elucidated the mechanisms underlying these effects. Our results showed that proliferating cell nuclear antigen (PCNA), Akt, and phosphoinositide 3-kinase (PI3K) were expressed mainly in ovarian oocytes and granulosa cells (GCs). We observed that 5 ng/mL Se significantly stimulated LGC proliferation, which could be attributed to increases in PCNA, cyclin-dependent kinase 1 (CDK1), phosphorylated adenosine monophosphate-activated protein kinase (p-AMPK; Thr172), and phosphor...
Source: Theriogenology - March 20, 2018 Category: Genetics & Stem Cells Authors: Xiaolei Yao, M.A. EI-Samahy, Lijie Fan, Linfeng Zheng, Yuyue Jin, Jing pang, Guomin Zhang, Zifei Liu, Feng Wang Source Type: research
Influence of polygamous versus monogamous mating on embryo production in four different strains of mice after superovulatory treatment
We determined the effect of monogamous or polygamous mating with 2 females on vaginal plug (VP) rate, embryo donors (ED), 2-cell embryo production, and male performance after superovulation of females aging 24d or 45-48d. C57BL/6NCrl (B6N), BALB/cAnCrl (BALB/cN), FVB/NCrl (FVB/N), and Crl:CD1(ICR) (CD-1) females received 5 IU eCG and 5 IU hCG (24d) or 7.5 IU eCG and 7.5 IU hCG (45-48d) 48 h apart. After the hCG injection, females were paired with males, which alternated weekly in monogamous or polygamous mating. (Source: Theriogenology)
Source: Theriogenology - March 17, 2018 Category: Genetics & Stem Cells Authors: Malte Heykants, Hagen Scherb, Geert Michel, Esther Mahabir Source Type: research
Systemic profiling of ectopic fat deposits in the reproductive tract of dairy cows
During the transition period, high-yielding dairy cows suffer from negative energy balance, intense lipomobilization and impaired lipid metabolism; this metabolic condition can lead to overburdened triglycerides accumulation in the liver, known as liver lipidosis, which has been associated to impaired fertility in dairy cows. The mechanisms of this impairment can be in principle correlated with the presence and the extent of ectopic fat depots. However, current methods for evaluating fat accumulation in liver and in the reproductive tract suffer from low resolution, sensitivity, and specificity. (Source: Theriogenology)
Source: Theriogenology - March 17, 2018 Category: Genetics & Stem Cells Authors: Martina Crociati, Flavio Di Giacinto, Elisabetta Manuali, Giuseppe Stradaioli, Lakamy Sylla, Maurizio Monaci, Giuseppe Maulucci, Marco De Spirito Source Type: research
Influence of polygamous versus monogamous mating on embryo production in four different strains of mice after superovulatory treatment
We determined the effect of monogamous or polygamous mating with 2 females on vaginal plug (VP) rate, embryo donors (ED), 2-cell embryo production, and male performance after superovulation of females aging 24d or 45-48d. C57BL/6NCrl (B6N), BALB/cAnCrl (BALB/cN), FVB/NCrl (FVB/N), and Crl:CD1(ICR) (CD-1) females received 5 IU eCG and 5 IU hCG (24d) or 7.5 IU eCG and 7.5 IU hCG (45-48d) 48 h apart. After the hCG injection, females were paired with males, which alternated weekly in monogamous or polygamous mating. (Source: Theriogenology)
Source: Theriogenology - March 17, 2018 Category: Genetics & Stem Cells Authors: Malte Heykants, Hagen Scherb, Geert Michel, Esther Mahabir Source Type: research
Systemic profiling of ectopic fat deposits in the reproductive tract of dairy cows
During the transition period, high-yielding dairy cows suffer from negative energy balance, intense lipomobilization and impaired lipid metabolism; this metabolic condition can lead to overburdened triglycerides accumulation in the liver, known as liver lipidosis, which has been associated to impaired fertility in dairy cows. The mechanisms of this impairment can be in principle correlated with the presence and the extent of ectopic fat depots. However, current methods for evaluating fat accumulation in liver and in the reproductive tract suffer from low resolution, sensitivity, and specificity. (Source: Theriogenology)
Source: Theriogenology - March 17, 2018 Category: Genetics & Stem Cells Authors: Martina Crociati, Flavio Di Giacinto, Elisabetta Manuali, Giuseppe Stradaioli, Lakamy Sylla, Maurizio Monaci, Giuseppe Maulucci, Marco De Spirito Source Type: research
Plasma IGF-I, INSL3, testosterone, inhibin concentrations and scrotal circumferences surrounding puberty in Japanese Black beef bulls with normal and abnormal semen
The relationships between semen abnormalities and peripheral concentrations of testicular and metabolic hormones in beef bulls are unclear. Here we compared plasma insulin-like growth factor I (IGF-I), insulin-like peptide 3 (INSL3), testosterone, inhibin concentrations, and scrotal circumferences surrounding puberty in Japanese Black beef bulls (n = 66) with normal or abnormal semen. We collected blood samples and measured scrotal circumferences monthly from 4 to 24 months of age. Semen was collected weekly from 12 months until at least 18 months of age. (Source: Theriogenology)
Source: Theriogenology - March 15, 2018 Category: Genetics & Stem Cells Authors: W.W.P.N. Weerakoon, M. Sakase, N. Kawate, M.A. Hannan, N. Kohama, H. Tamada Source Type: research
An in vitro study on noradrenergic modulation of final oocyte maturation in the catfish Heteropneustes fossilis
This study was aimed to elucidate in vitro the effects of endogenous catecholamines: dopamine (DA), noradrenaline (NA) and adrenaline (A), and the β-adrenergic blocker propranolol on induction of final oocyte maturation (FOM) in the catfish Heteropneustes fossilis. With this aim, post vitellogenic follicles from sexually mature grav id female catfish were incubated with each of DA, NA and A in a concentration range of 5–250 μM, and propranolol in a concentration range of 1–200 μg/mL at time points varying from 0 to 30 h). (Source: Theriogenology)
Source: Theriogenology - March 15, 2018 Category: Genetics & Stem Cells Authors: Abha Mishra, Radha Chaube, K.P. Joy Source Type: research
Immunocastration of goats using anti-gonadotrophin releasing hormone vaccine
The aim of this study was to evaluate the immuno-sterilizing action of anti-gonadotrophin-releasing hormone (anti-GnRH) vaccine in goats. Eighteen male goats were randomly distributed to receive three treatments: T1 (control) - whole animals, and T2 and T3 - application of 0.5 and 1.0 mL of anti-GnRH vaccine, respectively, with six replicates and one goat per experimental unit. Vaccine was administered at 8 months of age and 30 days after the first immunization. Testicular biometry was evaluated monthly, along with seminal collections, for the physical and morphological evalua tion of semen. (Source: Theriogenology)
Source: Theriogenology - March 15, 2018 Category: Genetics & Stem Cells Authors: Maicon Pereira Lents, Larissa Pires Barbosa, Ana L úcia Almeida Santana, Emmanuel Emydio Gomes Pinheiro, Lopes César Mugabe, Carmo Emanuel Almeida Biscarde, Cláudia Kazumi Kiya, William Morais Machado, Rosileia Silva Souza Source Type: research
Quantifying known and emerging uterine pathogens, and evaluating their association with metritis and fever in dairy cows
This study aims to quantify known and emerging uterine pathogens, and to evaluate their association with metritis and fever in dairy cows. Metritis was diagnosed at 6 ± 2 days postpartum, a uterine swab was collected and rectal temperature was measured. (Source: Theriogenology)
Source: Theriogenology - March 15, 2018 Category: Genetics & Stem Cells Authors: Federico Cunha, Soo Jin Jeon, Rodolfo Daetz, Achilles Vieira-Neto, Jimena Laporta, K. Casey Jeong, Anthony F. Barbet, Carlos A. Risco, Klibs N. Galv ão Source Type: research
Proliferative and apoptotic changes in the healthy canine endometrium and in cystic endometrial hyperplasia
Proliferation and apoptosis play an important role in cyclic changes in the healthy canine endometrium. Proteins of the Bcl-2-family are known to be involved in the regulation of apoptosis. However, only few reports mention their expression patterns during cystic endometrial hyperplasia (CEH). In order to correlate proliferative and apoptotic processes, expression of the apoptosis-regulatory proteins Bcl-2 (anti-apoptotic) and Bax (pro-apoptotic) in healthy and cystic hyperplastic endometrial tissue as well as in pyometra was investigated. (Source: Theriogenology)
Source: Theriogenology - March 15, 2018 Category: Genetics & Stem Cells Authors: N. Reusche, A. Beineke, C. Urhausen, M. Beyerbach, M. Schmicke, S. Kramer, A.R. G ünzel-Apel Source Type: research
Interferon- τ regulates prostaglandin release in goat endometrial stromal cells via JAB1 - unfolded protein response pathway
Prostaglandins (PGs) are major products of the uterine endometrium, and they are critical for recognition of pregnancy in ruminants. During the peri-implantation period of pregnancy, interferon tau (IFN- τ) plays an important role in the regulation of endometrial PGs synthesis, but the underlying mechanisms remain poorly understood. In this work, the results demonstrated that IFN-τ increased the PGE2/PGF2α ratio, up-regulated the expression of JAB1 and activated the unfolded protein response (UPR ). (Source: Theriogenology)
Source: Theriogenology - March 15, 2018 Category: Genetics & Stem Cells Authors: Diqi Yang, Tingting Jiang, Jianguo Liu, Jin Hong, Pengfei Lin, Huatao Chen, Dong Zhou, Keqiong Tang, Aihua Wang, Yaping Jin Source Type: research
Expression patterns and role of SDF-1/CXCR4 axis in boar spermatogonial stem cells
The signaling of chemokine stromal cell-derived factor (SDF)-1 and its receptor C-X-C motif chemokine receptor 4 (CXCR4) is involved in the cellular proliferation, survival, and migration of various cell types. Although SDF-1/CXCR4 has been implicated in the maintenance of the spermatogonial population during mouse testis development, their expression patterns and functions in boar testis remain unclear. In the present study, the expression pattern of SDF-1 and CXCR4 was determined during pre-pubertal and post-pubertal stage boar testes and in vitro cultured porcine spermatogonial stem cells (pSSCs). (Source: Theriogenology)
Source: Theriogenology - March 15, 2018 Category: Genetics & Stem Cells Authors: Hyun Jung Park, Won-Yong Lee, Jin Hoi Kim, Chankyu Park, Hyuk Song Source Type: research
Capsaicin protects against testicular torsion injury through mTOR-dependent mechanism
Testicular torsion and subsequent release of reactive oxygen species (ROS) can cause infertility in adults. Oxidative stress following testicular torsion plays an important role in the ýonset and development of apoptotic cell death through dysregulation of the cellular signaling pathways. Anti-inflammatory and antioxidant properties of capsaicin, a bioactive composition present in red peppers, has already been exploited for treatment of the cancer and pain relief. In present work , we evaluated the role of the mammalian target of rapamycin (mTOR) in antioxidant effect of capsaicin against reperfusion injury following test...
Source: Theriogenology - March 15, 2018 Category: Genetics & Stem Cells Authors: Nasim Javdan, Seyed Abdulmajid Ayatollahi, Muhammad Iqbal Choudhary, Safaa Al-Hasani, Farzad Kobarfard, Ata Athar, Hamidreza Pazoki-Toroudi Source Type: research
Plasma IGF-I, INSL3, testosterone, inhibin concentrations and scrotal circumferences surrounding puberty in Japanese Black beef bulls with normal and abnormal semen
The relationships between semen abnormalities and peripheral concentrations of testicular and metabolic hormones in beef bulls are unclear. Here we compared plasma insulin-like growth factor I (IGF-I), insulin-like peptide 3 (INSL3), testosterone, inhibin concentrations, and scrotal circumferences surrounding puberty in Japanese Black beef bulls (n = 66) with normal or abnormal semen. We collected blood samples and measured scrotal circumferences monthly from 4 to 24 months of age. Semen was collected weekly from 12 months until at least 18 months of age. (Source: Theriogenology)
Source: Theriogenology - March 15, 2018 Category: Genetics & Stem Cells Authors: W.W.P.N. Weerakoon, M. Sakase, N. Kawate, M.A. Hannan, N. Kohama, H. Tamada Source Type: research
Interferon- τ regulates prostaglandin release in goat endometrial stromal cells via JAB1 - unfolded protein response pathway
Prostaglandins (PGs) are major products of the uterine endometrium, and they are critical for recognition of pregnancy in ruminants. During the peri-implantation period of pregnancy, interferon tau (IFN- τ) plays an important role in the regulation of endometrial PGs synthesis, but the underlying mechanisms remain poorly understood. In this work, the results demonstrated that IFN-τ increased the PGE2/PGF2α ratio, up-regulated the expression of JAB1 and activated the unfolded protein response (UPR ). (Source: Theriogenology)
Source: Theriogenology - March 15, 2018 Category: Genetics & Stem Cells Authors: Diqi Yang, Tingting Jiang, Jianguo Liu, Jin Hong, Pengfei Lin, Huatao Chen, Dong Zhou, Keqiong Tang, Aihua Wang, Yaping Jin Source Type: research
