Short term protein supplementation during a long interval prostaglandin-based protocol for timed AI in sheep
The aim of this study was to evaluate the reproductive impact of a short-term protein supplementation on a long interval prostaglandin-based protocol (two PG injections 15 d apart; PG15) for timed artificial insemination in sheep. During the breeding season, 437 multiparous Merino ewes grazing native pastures (forage allowance of 6 kg of dry matter/100 kg of live weight; crude protein: 10.8%, metabolic energy: 2.1 Mcal/kg of dry matter) were selected. Ewes were a llocated, according to body condition score (3.2 ± 0.2) and body weight (40.6 ± 4.9 kg, mean ± SD), to a 2 × 2 factorial design: type of estrus ...
Source: Theriogenology - March 22, 2018 Category: Genetics & Stem Cells Authors: N. Errandonea, S. Fierro, C. Vi ñoles, J. Gil, G. Banchero, J. Olivera-Muzante Source Type: research
Effects of natural infection by L. borgpetersenii serovar Hardjo type Hardjo-bovis, L. interrogans serovar Pomona and leptospiral vaccination on sheep reproduction
This study evaluated the impact of exposure to these serovars and of vaccination, on primiparous one- (P1) and two-year-old (P2) sheep reproduction outcomes. The study was designed as a split-flock vaccination trial, with a third of the animals vaccinated starting at one month of age. Reproduction outcomes were the proportion of bred P1 (7 months old) and as P2 (19 months old) scanned pregnant, the proportion of pregnant ewes rearing a lamb to tail docking and the proportion of docked lambs that were weaned. (Source: Theriogenology)
Source: Theriogenology - March 22, 2018 Category: Genetics & Stem Cells Authors: Emilie Vall ée, Cord Heuer, Julie M. Collins-Emerson, Jackie Benschop, Anne L. Ridler, Peter R. Wilson Source Type: research
Proteomic content of circulating exosomes in dairy cows with or without uterine infection
In the past few decades, there has been a global decrease in dairy cow reproductive performance. An activated inflammatory system, due to uterine infection, has been associated with decreased cow fertility and as such, there is a need to detect uterine disease earlier. Early detection could be achieved by identifying biomarkers for uterine disease. Exosomes are small nanovesicles known to package and deliver protein, mRNA, and miRNAs to near and distant sites. Therefore, the content of circulating exosomes may have the potential to carry biomarkers for earlier diagnosis of disease. (Source: Theriogenology)
Source: Theriogenology - March 22, 2018 Category: Genetics & Stem Cells Authors: Fatema B. Almughlliq, Yong Q. Koh, Hassendrini N. Peiris, Kanchan Vaswani, Scott McDougall, Elizabeth M. Graham, Chris R. Burke, Buddhika J. Arachchige, Sarah Reed, Murray D. Mitchell Source Type: research
In vitro sperm quality and DNA integrity of SexedULTRA™ sex-sorted sperm compared to non-sorted bovine sperm
SexedULTRA ™ is an improved method of sex-sorting sperm creating a less damaging environment to retain sperm integrity through the sorting process. The aim of this study was to evaluate the in vitro characteristics of fresh and frozen bovine sperm using the SexedULTRA™ method, and compare it to convention al (non-sorted) sperm. For both methods, percent total sperm motility was estimated visually and also classified into total and progressively motile using a computer assisted sperm analyzer (CASA). (Source: Theriogenology)
Source: Theriogenology - March 22, 2018 Category: Genetics & Stem Cells Authors: C. Gonz ález-Marín, C.E. Góngora, T.B. Gilligan, K.M. Evans, J.F. Moreno, R. Vishwanath Source Type: research
Bovine uterus-derived exosomes improve developmental competence of somatic cell nuclear transfer embryos
Exosomes widely exist in various tissues and body fluids, including blood, tissue fluid, and urine. In the present study, exosomes were first isolated from the early luteal phase uterus and confirmed through morphological examination, immunofluorescence (IF) staining of special membrane antigen, and Western blot. The effects of exosomes on the developmental competence of somatic cell nuclear transfer (SCNT) embryos were investigated. Transmission electron microscopy results showed that the isolated exsomes were spherical particles with a 50 nm–150 nm diameter. (Source: Theriogenology)
Source: Theriogenology - March 22, 2018 Category: Genetics & Stem Cells Authors: Fang Qiao, Hui Ge, Xiaonan Ma, Ying Zhang, Zhenzi Zuo, Mengyun Wang, Yong Zhang, Yongsheng Wang Source Type: research
The anti-inflammatory effect of exogenous lactoferrin on breeding-induced endometritis in susceptible mares when administered post-breeding in susceptible mares
The deposition of semen into the uterus of the horse induces a transient innate immune response that lasts 24 –36 h in the normal mare. There exists a subset of mares that are unable to resolve this inflammation in a timely manner, and are classified as susceptible to the disease of persistent breeding-induced endometritis (PBIE). Lactoferrin is a protein of interest as a potential therapeutic for this persistent inflammation due to its anti-inflammatory and bactericidal properties. The addition of human recombinant lactoferrin (hrLF) to the insemination dose was previously shown to suppress mRNA expression of the pro-...
Source: Theriogenology - March 22, 2018 Category: Genetics & Stem Cells Authors: C.E. Fedorka, K.E. Scoggin, Y.L. Boakari, N.E. Hoppe, E.L. Squires, B.A. Ball, M.H.T. Troedsson Source Type: research
Effects of natural infection by L. borgpetersenii serovar Hardjo type Hardjo-bovis, L. interrogans serovar Pomona and leptospiral vaccination on sheep reproduction
This study evaluated the impact of exposure to these serovars and of vaccination, on primiparous one- (P1) and two-year-old (P2) sheep reproduction outcomes. The study was designed as a split-flock vaccination trial, with a third of the animals vaccinated starting at one month of age. Reproduction outcomes were the proportion of bred P1 (7 months old) and as P2 (19 months old) scanned pregnant, the proportion of pregnant ewes rearing a lamb to tail docking and the proportion of docked lambs that were weaned. (Source: Theriogenology)
Source: Theriogenology - March 22, 2018 Category: Genetics & Stem Cells Authors: Emilie Vall ée, Cord Heuer, Julie M. Collins-Emerson, Jackie Benschop, Anne L. Ridler, Peter R. Wilson Source Type: research
Proteomic content of circulating exosomes in dairy cows with or without uterine infection
In the past few decades, there has been a global decrease in dairy cow reproductive performance. An activated inflammatory system, due to uterine infection, has been associated with decreased cow fertility and as such, there is a need to detect uterine disease earlier. Early detection could be achieved by identifying biomarkers for uterine disease. Exosomes are small nanovesicles known to package and deliver protein, mRNA, and miRNAs to near and distant sites. Therefore, the content of circulating exosomes may have the potential to carry biomarkers for earlier diagnosis of disease. (Source: Theriogenology)
Source: Theriogenology - March 22, 2018 Category: Genetics & Stem Cells Authors: Fatema B. Almughlliq, Yong Q. Koh, Hassendrini N. Peiris, Kanchan Vaswani, Scott McDougall, Elizabeth M. Graham, Chris R. Burke, Buddhika J. Arachchige, Sarah Reed, Murray D. Mitchell Source Type: research
Bovine uterus-derived exosomes improve developmental competence of somatic cell nuclear transfer embryos
Exosomes widely exist in various tissues and body fluids, including blood, tissue fluid, and urine. In the present study, exosomes were first isolated from the early luteal phase uterus and confirmed through morphological examination, immunofluorescence (IF) staining of special membrane antigen, and Western blot. The effects of exosomes on the developmental competence of somatic cell nuclear transfer (SCNT) embryos were investigated. Transmission electron microscopy results showed that the isolated exsomes were spherical particles with a 50 nm–150 nm diameter. (Source: Theriogenology)
Source: Theriogenology - March 22, 2018 Category: Genetics & Stem Cells Authors: Fang Qiao, Hui Ge, Xiaonan Ma, Ying Zhang, Zhenzi Zuo, Mengyun Wang, Yong Zhang, Yongsheng Wang Source Type: research
Reference range of gestational uterine artery resistance index in small canine breeds
The objectives of this study were to evaluate the uterine artery blood flow during the second half of normal pregnancy in small breed dogs and to determine the RI reference values of this artery in these bitches. Fifty-two, 1.5 –6.5 kg, purebred healthy pregnant bitches were included in this study. Doppler ultrasonographic examinations of the uterus were performed every 10 days from Day 30 of the estrous cycle to parturition. Peak systolic velocity (PSV) and end diastolic velocity (EDV) of the uterine artery were measur ed while RI [(PSV−EDV)/PSV] was automatically calculated. (Source: Theriogenology)
Source: Theriogenology - March 22, 2018 Category: Genetics & Stem Cells Authors: P.R. Batista, C. Gobello, A. Rube, J.P. Barrena, N.E. Re, P.G. Blanco Source Type: research
Angiocoupling between the dominant follicle and corpus luteum during waves 1 and 2 in Bos taurus heifers
The local two-way effect of the future and established dominant follicle (DF) and corpus luteum (CL) on color-Doppler signals of blood flow in each other (angiocoupling) was studied from ovulation to 16 days after ovulation during wave 1 and for the first time in wave 2 in Bos taurus heifers (N = 24). Identity of follicles greater than 4 mm was maintained from day to day. Blood-flow signals in the follicle wall and in the CL were estimated after scanning the entire structure. Ovarian patterns were defined based on the presence of a DF and CL: DF−CL pattern (DF and CL in the same o vary), DF pattern or CL pattern (...
Source: Theriogenology - March 22, 2018 Category: Genetics & Stem Cells Authors: R.R. Domingues, O.J. Ginther Source Type: research
Hormonal strategy to reduce suckled beef cow handling for timed artificial insemination with sex-sorted semen
Two experiments were conducted to assess a hormonal strategy developed to reduce animal handling for timed artificial insemination (TAI) with sex-sorted semen. Four-hundred ninety-one (491) suckled beef cows received a progesterone (P4) intravaginal device and 2 mg intramuscular (im) injection of estradiol benzoate (EB) on a randomly chosen day of the estrus cycle (Day 0) in Experiment 1. Cows were treated with 500 μg of sodic cloprostenol (PGF2α) and with 300 IU of eCG at P4 device removal (Day 8); these cows were also randomly assigned to receive 1 mg of estradiol cypionate (EC) administered at P4 device remova...
Source: Theriogenology - March 22, 2018 Category: Genetics & Stem Cells Authors: M.A.V. Silva, C.S. Santos, I.G. Fran ça, H.G. Pereira, M.F. Sá Filho, B.G. Freitas, B.M. Guerreiro, A. Faquim, P.S. Baruselli, J.R.S. Torres-Júnior Source Type: research
Short term protein supplementation during a long interval prostaglandin-based protocol for timed AI in sheep
The aim of this study was to evaluate the reproductive impact of a short-term protein supplementation on a long interval prostaglandin-based protocol (two PG injections 15 d apart; PG15) for timed artificial insemination in sheep. During the breeding season, 437 multiparous Merino ewes grazing native pastures (forage allowance of 6 kg of dry matter/100 kg of live weight; crude protein: 10.8%, metabolic energy: 2.1 Mcal/kg of dry matter) were selected. Ewes were a llocated, according to body condition score (3.2 ± 0.2) and body weight (40.6 ± 4.9 kg, mean ± SD), to a 2 × 2 factorial design: type of estrus ...
Source: Theriogenology - March 22, 2018 Category: Genetics & Stem Cells Authors: N. Errandonea, S. Fierro, C. Vi ñoles, J. Gil, G. Banchero, J. Olivera-Muzante Source Type: research
In vitro sperm quality and DNA integrity of SexedULTRA ™ sex-sorted sperm compared to non-sorted bovine sperm
SexedULTRA ™ is an improved method of sex-sorting sperm creating a less damaging environment to retain sperm integrity through the sorting process. The aim of this study was to evaluate the in vitro characteristics of fresh and frozen bovine sperm using the SexedULTRA™ method, and compare it to conventiona l (non-sorted) sperm. For both methods, percent total sperm motility was estimated visually and also classified into total and progressively motile using a computer assisted sperm analyzer (CASA). (Source: Theriogenology)
Source: Theriogenology - March 22, 2018 Category: Genetics & Stem Cells Authors: C. Gonz ález-Marín, C.E. Góngora, T.B. Gilligan, K.M. Evans, J.F. Moreno, R. Vishwanath Source Type: research
In vitro influence of selenium on the proliferation of and steroidogenesis in goat luteinized granulosa cells
In this study, we investigated the effects of Selenium (Se) on the proliferation of and steroidogenesis in goat luteinized granulosa cells (LGCs) and elucidated the mechanisms underlying these effects. Our results showed that proliferating cell nuclear antigen (PCNA), Akt, and phosphoinositide 3-kinase (PI3K) were expressed mainly in ovarian oocytes and granulosa cells (GCs). We observed that 5 ng/mL Se significantly stimulated LGC proliferation, which could be attributed to increases in PCNA, cyclin-dependent kinase 1 (CDK1), phosphorylated adenosine monophosphate-activated protein kinase (p-AMPK; Thr172), and phosphor...
Source: Theriogenology - March 20, 2018 Category: Genetics & Stem Cells Authors: Xiaolei Yao, M.A. EI-Samahy, Lijie Fan, Linfeng Zheng, Yuyue Jin, Jing pang, Guomin Zhang, Zifei Liu, Feng Wang Source Type: research
