In  vitro influence of selenium on the proliferation of and steroidogenesis in goat luteinized granulosa cells

In this study, we investigated the effects of Selenium (Se) on the proliferation of and steroidogenesis in goat luteinized granulosa cells (LGCs) and elucidated the mechanisms underlying these effects. Our results showed that proliferating cell nuclear antigen (PCNA), Akt, and phosphoinositide 3-kinase (PI3K) were expressed mainly in ovarian oocytes and granulosa cells (GCs). We observed that 5  ng/mL Se significantly stimulated LGC proliferation, which could be attributed to increases in PCNA, cyclin-dependent kinase 1 (CDK1), phosphorylated adenosine monophosphate-activated protein kinase (p-AMPK; Thr172), and phosphorylated Akt (p-Akt; Ser473) and decreases in p21 (P 

Source: Theriogenology - March 20, 2018 Category: Genetics & Stem Cells Authors: Xiaolei Yao, M.A. EI-Samahy, Lijie Fan, Linfeng Zheng, Yuyue Jin, Jing pang, Guomin Zhang, Zifei Liu, Feng Wang Source Type: research