Quantitative proteomic profiling indicates the difference in reproductive efficiency between Meishan and Duroc boar spermatozoa
The reproductive efficiency of Meishan pigs is higher than that of Duroc pigs, but the underlying molecular mechanism for this disparity remains unclear. No systematic quantitative proteomics studies, comparing global proteins in Meishan and Duroc boar spermatozoa have been reported. Therefore, we applied iTRAQ labeling coupled with mass spectrometry, and analyzed the differences in proteins between Meishan and Duroc sperm. In the present study, a total of 1597 proteins were quantified. Of these proteins, 190 showed statistically significant fold changes between Meishan and Duroc spermatozoa. (Source: Theriogenology)
Source: Theriogenology - April 30, 2018 Category: Genetics & Stem Cells Authors: Li Xinhong, Linqing Zhen, Jieli Fu, Lirui Wang, Qiangzhen Yang, Peifei Li, Yuhua Li Source Type: research
Delayed return to estrus following treatment with the gonadotrophin-releasing hormone agonist, Lucrin ® Depot, in the tammar wallaby
Gonadotrophin-releasing hormone agonists that induce a reversible contraceptive state in several marsupials have the potential to be used to synchronize estrus. We used a model macropod, the tammar wallaby (Notamacropus eugenii), to investigate whether Lucrin Depot (AbbVie), a GnRH agonist microsphere preparation, could (1) inhibit follicular development and estrus in a cycle reactivated by removal of pouch young (RPY) and (2) facilitate a synchronous return to estrus. Our results show that females reactivated with bromocriptine and RPY in early seasonal quiescence (July 2015) were inhibited by Lucrin Depot (0.125 –0.5...
Source: Theriogenology - April 30, 2018 Category: Genetics & Stem Cells Authors: Ryan R. Witt, Lyn A. Hinds, John C. Rodger Source Type: research
Biochemical blood analysis along pregnancy in Martina Franca jennies
The availability of biochemical blood data specific for the pregnant condition are essential for the correct management of both normal pregnancies and also for the prompt recognition of every abnormality. Because of the lacking knowledge about biochemical blood analysis in the donkey along the entire pregnancy, the study was designed to provide first preliminary data about the values and possible changes of blood alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL), alkaline phosphatase (ALP), creatine-kinase (CK), blood urea nitrogen (BUN), creatinine (CREA), uric acid (UA), amylase (AM...
Source: Theriogenology - April 28, 2018 Category: Genetics & Stem Cells Authors: Alessia Gloria, Maria Cristina Veronesi, Rita Carluccio, Salvatore Parrillo, Ippolito De Amicis, Alberto Contri Source Type: research
Endoscopy-mediated intratubal insemination in the cow - development of a novel minimally invasive AI technique
Conventionally inseminated spermatozoa suffer a dramatic reduction in numbers during their long journey until fertilization. In addition sperm survival seems to be strongly affected by the reconstitution of the female reproductive tract in the post partum period. The purpose of this study was to develop a novel AI technique for cattle that allows the deposition of spermatozoa directly into the ampulla in the immediate vicinity of the fertilization site. This new reproductive biotechnique was investigated with focus on semen origin, sperm dosage, semen preparation and time of insemination. (Source: Theriogenology)
Source: Theriogenology - April 27, 2018 Category: Genetics & Stem Cells Authors: Karina Radefeld, Sophie Papp, Vitezslav Havlicek, Jane M. Morrell, Gottfried Brem, Urban Besenfelder Source Type: research
Subclinical mastitis interferes with ovulation, oocyte and granulosa cell quality in dairy cows
The objective was to evaluate the effect of mastitis by somatic cell count (SCC) on follicular growth, ovulation, oocytes and cumulus cells quality and on the concentration and size of exosomes in follicular fluid of dairy cows. In the study, crossbred cows (Bos taurus – Holstein x Bos indicus – Gir) were classified for analysis as Control (SCC400.000 cells/mL) groups. In experiment 1 (follicular dynamics), cows (n=57) were submitted to ultrasound evaluations every 24 h, from progesterone-releasing-intravaginal-device (PRID) removal (D8) until 48 h later (D10). (Source: Theriogenology)
Source: Theriogenology - April 27, 2018 Category: Genetics & Stem Cells Authors: G. Santos, M.P. Bottino, A.P.C. Santos, L.M.S. Sim ões, J.C. Souza, M.B.D. Ferreira, J.C. da Silveira, A.C.F.C.M. Ávila, A. Bride, J.N.S. Sales Source Type: research
Presynchronization by induction of a largest follicle using a progesterone device in GnRH-based-ovulation synchronization protocol in crossbred dairy cows
The objective was to compare the fertility of dairy cows using a presynchronization protocol by induction of a largest follicle using a progesterone intravaginal device prior to an Ovsynch protocol (P4synch) with the Double-Ovsynch in lactating dairy cows. Lactating Bos indicus x Bos taurus crossbred cows (n=440) were randomly allocated to one of two treatments: (I) Double-Ov (n=228), GnRH (D-17), PGF2 α 7 days later (D-10) and GnRH 3 days later (D-7) followed by an Ovsynch protocol 7 days later (GnRH on D0, PGF on D7, GnRH on D9); (II) P4synch (n=212), insertion of a sustained release progesterone intravaginal device (D-...
Source: Theriogenology - April 27, 2018 Category: Genetics & Stem Cells Authors: L.A.C.L. Silva, L.M.S. Sim ões, M.P. Bottino, A.P.C. Santos, G. Santos, I.Y.H. Martinez, J.C. Souza, P.S. Baruselli, J.N.S. Sales Source Type: research
Sequential concentrations of placental growth factor and haptoglobin, and their relation to oestrone sulphate and progesterone in pregnant Spanish Purebred mare
The objectives of this study were to establish reference values for serum concentrations of placental growth factor (PlGF) and haptoglobin (Hp), and to analyze whether the levels of oestrone sulphate (E1S) and progesterone (P4) are physiologically involved in the dynamic modifications of the above parameters in pregnant mares. A total of 30 healthy Spanish Purebred mares ranging in age 9.33 ± 3.31 years were studied during the 11 months of gestation. Serum concentrations of PlGF were detected by EIA, Hp using commercial Phase Haptoglobin assay and E1S and P4 levels through RIA. (Source: Theriogenology)
Source: Theriogenology - April 27, 2018 Category: Genetics & Stem Cells Authors: K. Satu é, M. Marcilla, P. Medica, A. Ferlazzo, E. Fazio Source Type: research
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Source: Theriogenology - April 26, 2018 Category: Genetics & Stem Cells Source Type: research
Seminal plasma influences the fertilizing potential of cryopreserved stallion sperm
The objective of this study was to investigate the effect of adding pooled SP from “good” (GF) or “bad” (BF) freezer stallions on sperm cells' fertilizing ability. “Good freezers” refers to stallions that usually produce ejaculates which can withstand cryopreservation, whilst “bad freezer” stallions produce ejaculates which cannot tolerate the freezing process. (Source: Theriogenology)
Source: Theriogenology - April 26, 2018 Category: Genetics & Stem Cells Authors: Essraa M Al-Essawe, Margareta Wallgren, Manuela Wulf, Christine Aurich, Beatriz Mac ías-García, Ylva Sjunnesson, Jane M. Morrell Source Type: research
Seminal plasma influences the fertilizing potential of cryopreserved stallion sperm cells
The objective of this study was to investigate the effect of adding pooled SP from “good” (GF) or “bad” (BF) freezer stallions on sperms cells´ fertilizing ability. “Good freezers” refers to stallions that usually produce ejaculates which can withstand cryopreservation, whilst “bad freezer” stallions produce ejaculates which cannot tolerate the freezing process. (Source: Theriogenology)
Source: Theriogenology - April 26, 2018 Category: Genetics & Stem Cells Authors: Essraa M Al-Essawe, Margareta Wallgren, Manuela Wulf, Christine Aurich, Beatriz Macias-Garcia, Ylva Sjunnesson, Jane M. Morrell Source Type: research
Horse embryo diameter is influenced by the embryonic age but not by the type of semen used to inseminate donor mares
The diameter of embryos recovered from mares on Day 8 after ovulation varies greatly, from as little as 130 μm to as much as 2500 μm. Several factors have been proposed to affect embryo size at recovery, one of which is the type of semen (frozen vs fresh or cooled-transported) used to inseminate the mare. In addition, it has been shown that smaller embryos ( (Source: Theriogenology)
Source: Theriogenology - April 26, 2018 Category: Genetics & Stem Cells Authors: J. Cuervo-Arango, A.N. Claes, T.A.E. Stout Source Type: research
Arterial blood flow is the main source of testicular heat in bulls and higher ambient temperatures significantly increase testicular blood flow
Two experiments were done in bulls to determine: total testicular blood flow, testis oxygenation and heat, and effects of ambient temperature on testicular temperatures and blood flow. In Experiment 1, arterial blood flow to testes and testicular oxygenation and heat were determined in Angus bulls (n = 8). Blood temperature and hemoglobin O2 saturation were both greater (P (Source: Theriogenology)
Source: Theriogenology - April 25, 2018 Category: Genetics & Stem Cells Authors: C.M.Q. Barros Adwell, L.F.C. Brito, E. Oba, R.E. Wilde, G. Rizzoto, J.C. Thundathil, J.P. Kastelic Source Type: research
Pressure shock triploidization of Salmo trutta f. lacustris and Salvelinus umbla eggs and its impact on fish development
The study tested the efficiency of hydrostatic pressure triploidization methods for Salmo trutta f. lacustris and Salvelinus umbla and investigated the effects on survival rate, skeletal malformation, and on morphometrics and cellular composition of gills, spleen, liver, kidney, intestine, and blood. In Salmo trutta f. lacustris a 100% triploidy rate in combination with high larvae survival rate (80% in comparison to control) was obtained when treating eggs with a pressure of 66 × 103 kPa 360 °C temperature minutes (CTM) post fertilization for 5 min, in Salvelinus umbla with a similar pressure after 270 CTM. (...
Source: Theriogenology - April 24, 2018 Category: Genetics & Stem Cells Authors: Franz Lahnsteiner, Manfred Kletzl Source Type: research
Effects of melatonin administration on seminal plasma metabolites and sperm fertilization competence during the non-reproductive season in ram
The purpose of this study was to investigate the effects of ram melatonin treatment on the sperm quality and metabolite composition of the seminal plasma in the non-breeding season. Four mature rams were treated with 54 mg melatonin in March subcutaneous implants and four untreated rams served as control. At 0, 30, 90 and 120 days semen samples were collected and sperm, separated from seminal plasma, was evaluated for its capacity to fertilize and produce embryos in vitro. Seminal plasma metabolites were extrac ted and analyzed by capillary electrophoresis/mass spectroscopy. (Source: Theriogenology)
Source: Theriogenology - April 23, 2018 Category: Genetics & Stem Cells Authors: Valentina Satta, Maria Elena Manca, Laura Torres-Rovira, Sara Succu, Paolo Mereu, Michella Nehme, Gianpaolo Epifani, Marilia Gallus, Fiammetta Berlinguer, Salvatore Naitana, Giovanni Giuseppe Leoni Source Type: research
