Pressure shock triploidization of Salmo trutta f. lacustris and Salvelinus umbla eggs and its impact on fish development

The study tested the efficiency of hydrostatic pressure triploidization methods for Salmo trutta f. lacustris and Salvelinus umbla and investigated the effects on survival rate, skeletal malformation, and on morphometrics and cellular composition of gills, spleen, liver, kidney, intestine, and blood. In Salmo trutta f. lacustris a 100% triploidy rate in combination with high larvae survival rate (80% in comparison to control) was obtained when treating eggs with a pressure of 66  × 103 kPa 360 °C temperature minutes (CTM) post fertilization for 5 min, in Salvelinus umbla with a similar pressure after 270 CTM.
Source: Theriogenology - Category: Genetics & Stem Cells Authors: Source Type: research