Expression patterns and role of SDF-1/CXCR4 axis in boar spermatogonial stem cells
The signaling of chemokine stromal cell-derived factor (SDF)-1 and its receptor C-X-C motif chemokine receptor 4 (CXCR4) is involved in the cellular proliferation, survival, and migration of various cell types. Although SDF-1/CXCR4 has been implicated in the maintenance of the spermatogonial population during mouse testis development, their expression patterns and functions in boar testis remain unclear. In the present study, the expression pattern of SDF-1 and CXCR4 was determined during pre-pubertal and post-pubertal stage boar testes and in vitro cultured porcine spermatogonial stem cells (pSSCs). (Source: Theriogenology)
Source: Theriogenology - March 15, 2018 Category: Genetics & Stem Cells Authors: Hyun Jung Park, Won-Yong Lee, Jin Hoi Kim, Chankyu Park, Hyuk Song Source Type: research
An in vitro study on noradrenergic modulation of final oocyte maturation in the catfish Heteropneustes fossilis
This study was aimed to elucidate in vitro the effects of endogenous catecholamines: dopamine (DA), noradrenaline (NA) and adrenaline (A), and the β-adrenergic blocker propranolol on induction of final oocyte maturation (FOM) in the catfish Heteropneustes fossilis. With this aim, post vitellogenic follicles from sexually mature gravid female catfish were incubated with each of DA, NA and A in a concentration range of 5–250 μM, and propra nolol in a concentration range of 1–200 μg/mL at time points varying from 0 to 30 h). (Source: Theriogenology)
Source: Theriogenology - March 15, 2018 Category: Genetics & Stem Cells Authors: Abha Mishra, Radha Chaube, K.P. Joy Source Type: research
Capsaicin protects against testicular torsion injury through mTOR-dependent mechanism
Testicular torsion and subsequent release of reactive oxygen species (ROS) can cause infertility in adults. Oxidative stress following testicular torsion plays an important role in the ýonset and development of apoptotic cell death through dysregulation of the cellular signaling pathways. Anti-inflammatory and antioxidant properties of capsaicin, a bioactive composition present in red peppers, has already been exploited for treatment of the cancer and pain relief. In present work , we evaluated the role of the mammalian target of rapamycin (mTOR) in antioxidant effect of capsaicin against reperfusion injury following test...
Source: Theriogenology - March 15, 2018 Category: Genetics & Stem Cells Authors: Nasim Javdan, Seyed Abdulmajid Ayatollahi, Muhammad Iqbal Choudhary, Safaa Al-Hasani, Farzad Kobarfard, Ata Athar, Hamidreza Pazoki-Toroudi Source Type: research
Immunocastration of goats using anti-gonadotrophin releasing hormone vaccine
The aim of this study was to evaluate the immuno-sterilizing action of anti-gonadotrophin-releasing hormone (anti-GnRH) vaccine in goats. Eighteen male goats were randomly distributed to receive three treatments: T1 (control) - whole animals, and T2 and T3 - application of 0.5 and 1.0 mL of anti-GnRH vaccine, respectively, with six replicates and one goat per experimental unit. Vaccine was administered at 8 months of age and 30 days after the first immunization. Testicular biometry was evaluated monthly, along with seminal collections, for the physical and morphological evalua tion of semen. (Source: Theriogenology)
Source: Theriogenology - March 15, 2018 Category: Genetics & Stem Cells Authors: Maicon Pereira Lents, Larissa Pires Barbosa, Ana L úcia Almeida Santana, Emmanuel Emydio Gomes Pinheiro, Lopes César Mugabe, Carmo Emanuel Almeida Biscarde, Cláudia Kazumi Kiya, William Morais Machado, Rosileia Silva Souza Source Type: research
Quantifying known and emerging uterine pathogens, and evaluating their association with metritis and fever in dairy cows
This study aims to quantify known and emerging uterine pathogens, and to evaluate their association with metritis and fever in dairy cows. Metritis was diagnosed at 6 ± 2 days postpartum, a uterine swab was collected and rectal temperature was measured. (Source: Theriogenology)
Source: Theriogenology - March 15, 2018 Category: Genetics & Stem Cells Authors: Federico Cunha, Soo Jin Jeon, Rodolfo Daetz, Achilles Vieira-Neto, Jimena Laporta, K. Casey Jeong, Anthony F. Barbet, Carlos A. Risco, Klibs N. Galv ão Source Type: research
Proliferative and apoptotic changes in the healthy canine endometrium and in cystic endometrial hyperplasia
Proliferation and apoptosis play an important role in cyclic changes in the healthy canine endometrium. Proteins of the Bcl-2-family are known to be involved in the regulation of apoptosis. However, only few reports mention their expression patterns during cystic endometrial hyperplasia (CEH). In order to correlate proliferative and apoptotic processes, expression of the apoptosis-regulatory proteins Bcl-2 (anti-apoptotic) and Bax (pro-apoptotic) in healthy and cystic hyperplastic endometrial tissue as well as in pyometra was investigated. (Source: Theriogenology)
Source: Theriogenology - March 15, 2018 Category: Genetics & Stem Cells Authors: N. Reusche, A. Beineke, C. Urhausen, M. Beyerbach, M. Schmicke, S. Kramer, A.R. G ünzel-Apel Source Type: research
Effect of vitrification solutions on survival rate of cryopreserved Epinephelus moara embryos
In this study, embryos of Epinephelus moara were used to investigate the effects of various cryopreservation methods. Embryos in stages 10 pairs somite (10S), 18 pairs somite (18S), 22 pairs somite (22S), tail-bud (TB), embryo twitching (ET) and pre-hatch (PH) were treated with five-step equilibrium penetration in 40% PMG3T vitrification solution, which contained 15.75% 1,2-propylene glycol, 10.50% Methanol, 8.75% Glycerol and 5.00% Trehalose. (Source: Theriogenology)
Source: Theriogenology - March 12, 2018 Category: Genetics & Stem Cells Authors: Y.S. Tian, J.J. Zhang, Z.T. Li, J. Tang, M.L. Cheng, Y.P. Wu, W.H. Ma, Z.F. Pang, W.S. Li, J.M. Zhai, B. Li Source Type: research
An overview of Jatropha curcas meal-induced productive and reproductive toxicity in Japanese quail: Potential mechanisms and heat detoxification
The present study was carried out to investigate the toxic effects of dietary inclusion of raw Jatropha cucas meal [RJM, 3.5%] on productive and reproductive performances of laying Japanese quail and the influence of physical treatment of jatropha meal by heating at [100 oc] for 24 or 48 h [JH24 or JH48 respectively] on reducing these effects. The inclusion of RJM in quail diets significantly decreased the egg production, fertility and hatchability percentages and resulted in a high mortality rate. (Source: Theriogenology)
Source: Theriogenology - March 12, 2018 Category: Genetics & Stem Cells Authors: Mayada R. Farag, Mahmoud Alagawany, Mohamed M. Badr, Samah R. Khalil, Mohamed S. El-Kholy Source Type: research
Effect of vitrification solutions on survival rate of cryopreserved Epinephelus moara embryos
In this study, embryos of Epinephelus moara were used to investigate the effects of various cryopreservation methods. Embryos in stages 10 pairs somite (10S), 18 pairs somite (18S), 22 pairs somite (22S), tail-bud (TB), embryo twitching (ET) and pre-hatch (PH) were treated with five-step equilibrium penetration in 40% PMG3T vitrification solution, which contained 15.75% 1,2-propylene glycol, 10.50% Methanol, 8.75% Glycerol and 5.00% Trehalose. (Source: Theriogenology)
Source: Theriogenology - March 12, 2018 Category: Genetics & Stem Cells Authors: Y.S. Tian, J.J. Zhang, Z.T. Li, J. Tang, M.L. Cheng, Y.P. Wu, W.H. Ma, Z.F. Pang, W.S. Li, J.M. Zhai, B. Li Source Type: research
An overview of Jatropha curcas meal induced productive and reproductive toxicity in Japanese quail: Potential mechanisms and heat detoxification
The present study was carried out to investigate the toxic effects of dietary inclusion of raw Jatropha cucas meal [RJM, 3.5%] on productive and reproductive performances of laying Japanese quail and the influence of physical treatment of jatropha meal by heating at [100 oc] for 24 or 48 h [JH24 or JH48 respectively] on reducing these effects. The inclusion of RJM in quail diets significantly decreased the egg production, fertility and hatchability percentages and resulted in a high mortality rate. (Source: Theriogenology)
Source: Theriogenology - March 12, 2018 Category: Genetics & Stem Cells Authors: Mayada R. Farag, Mahmoud Alagawany, Mohamed M. Badr, Samah R. Khalil, Mohamed S. El-Kholy Source Type: research
Variations in bovine embryo production between individual donors for OPU-IVF are closely related to glutathione concentrations in oocytes during in vitro maturation
Variations in embryo production between individual oocyte donors represent a serious problem in cattle production, when implementing ovum pick-up (OPU) and in vitro maturation (IVM) of oocytes. However, the precise cause of this problem is unknown. Here, we aimed to investigate the relationship between the glutathione (GSH) concentration in IVM oocytes and embryo development to explore a potential cause of the variations between individual donors. First , we found a high positive correlation (r = 0.80) between the GSH concentration in IVM oocytes and the blastocyst development rate of oocytes collected in the same OP...
Source: Theriogenology - March 9, 2018 Category: Genetics & Stem Cells Authors: Takemasa Hidaka, Yutaka Fukumoto, Satoshi Yamamoto, Yasuhiro Ogata, Toshitaka Horiuchi Source Type: research
Influence of insemination time on the fertility of sex sorted frozen-thawed Y-sperm in red deer
The aim of this study was to assess the effect of insemination timing on pregnancy rates in red deer (Cervus elaphus) when using sex-sorted sperm samples. Semen was collected by electroejaculation from 8 mature stags and processed to obtain: Conventional samples, following standard freezing procedures for commercial purposes; Control sorted samples, diluted and handled as per sorted samples but without being submitted to the sorter passage; and Y Sex Sorted (YSS) samples. Hinds were synchronized via intravaginal CIDR (Controlled Internal Drug Release) placement and given eCG (Folligon ® PMSG Serum Gonadotrophin) on day 12...
Source: Theriogenology - March 9, 2018 Category: Genetics & Stem Cells Authors: Luis Anel-L ópez, Olga Garcia-Álvarez, Tatiana Tarantini, David Del Olmo, Jose Antonio Ortiz, Sergio Ledda, Emilio Arsenio Martinez, Ana J. Soler, Jordi Roca, María R. Fernández Santos, Juan Maria Vázquez, Inmaculada Parrilla, Jose Julián Garde Source Type: research
Variations in bovine embryo production between individual donors for OPU-IVF are closely related to glutathione concentrations in oocytes during in vitro maturation
Variations in embryo production between individual oocyte donors represent a serious problem in cattle production, when implementing ovum-pick up (OPU) and in vitro maturation (IVM) of oocytes. However, the precise cause of this problem is unknown. Here, we aimed to investigate the relationship between the glutathione (GSH) concentration in IVM oocytes and embryo development to explore a potential cause of the variations between individual donors. First, we found a high positive correlation (r = 0.80) between the GSH concentration in IVM oocytes and the blastocyst development rate of oocytes collected in the same OPU ...
Source: Theriogenology - March 9, 2018 Category: Genetics & Stem Cells Authors: Takemasa Hidaka, Yutaka Fukumoto, Satoshi Yamamoto, Yasuhiro Ogata, Toshitaka Horiuchi Source Type: research
Influence of insemination time on the fertility of sex sorted frozen-thawed Y-sperm in red deer
The aim of this study was to assess the effect of insemination timing on pregnancy rates in red deer (Cervus elaphus) when using sex-sorted sperm samples. Semen was collected by electroejaculation from 8 mature stags and processed to obtain: Conventional samples, following standard freezing procedures for commercial purposes; Control sorted samples, diluted and handled as per sorted samples but without being submitted to the sorter passage; and Y Sex Sorted (YSS) samples. Hinds were synchronized via intravaginal CIDR (Controlled Internal Drug Release) placement and given eCG (Folligon ® PMSG Serum Gonadotrophin) on day 12...
Source: Theriogenology - March 9, 2018 Category: Genetics & Stem Cells Authors: Luis Anel-L ópez, Olga Garcia-Álvarez, Tatiana Tarantini, David Del Olmo, Jose Antonio Ortiz, Sergio Ledda, Emilio Arsenio Martinez, Ana J. Soler, Jordi Roca, María R. Fernández Santos, Juan Maria Vázquez, Inmaculada Parrilla, Jose Julián Garde Source Type: research
