Effect of vitrification solutions on survival rate of cryopreserved Epinephelus moara embryos

In this study, embryos of Epinephelus moara were used to investigate the effects of various cryopreservation methods. Embryos in stages 10 pairs somite (10S), 18 pairs somite (18S), 22 pairs somite (22S), tail-bud (TB), embryo twitching (ET) and pre-hatch (PH) were treated with five-step equilibrium penetration in 40% PMG3T vitrification solution, which contained 15.75% 1,2-propylene glycol, 10.50% Methanol, 8.75% Glycerol and 5.00% Trehalose.

Source: Theriogenology - March 12, 2018 Category: Genetics & Stem Cells Authors: Y.S. Tian, J.J. Zhang, Z.T. Li, J. Tang, M.L. Cheng, Y.P. Wu, W.H. Ma, Z.F. Pang, W.S. Li, J.M. Zhai, B. Li Source Type: research