miRNA-185 regulates the VEGFA signaling pathway in dairy cows with retained fetal membranes
Retention of fetal membranes (RFM) of cows is an important reproductive disturbance, and is related to miRNAs. Vascular endothelial growth factor (VEGF)A, regulated by miRNA-185, can activate arachidonic acid (ARA) release via the VEGFA signaling pathway, which influences RFM. The aim of this study was to explore the pathogenic mechanism of RFM by investigating the regulatory relationship between miRNA-185 and the VEGFA signaling pathway. Serum samples of healthy Holstein dairy cows (n = 20) and RFM cows (n = 12), with a similar age, parity, weight, and milk yield, were collected to detect VEGFA and ARA concentrations...
Source: Theriogenology - January 9, 2018 Category: Genetics & Stem Cells Authors: C.Y. Zheng, X. Zou, H.J. Lin, B.C. Zhao, M.L. Zhang, C.H. Luo, S.X. Fu Source Type: research
The relationship between external auditory canal temperature and onset of estrus and ovulation in beef heifers
The aim of this study was to evaluate the relationship of body temperature fluctuations, as measured by external auditory canal temperature, to the onset of estrus and ovulation. Beef heifers (n = 44, mean age 23.5 ± 0.4 months, mean weight 603.3 ± 5.7 kg) were fitted with a Boviminder® ear tag 2 weeks before the start of the estrous synchronization protocol to allow acclimatization. The device recorded the temperature, accurate to 0.01° Fahrenheit, every 10 min and tran smitted the data via a base station over the internet where it could be accessed remotely. (Source: Theriogenology)
Source: Theriogenology - January 9, 2018 Category: Genetics & Stem Cells Authors: Federico Randi, Michael McDonald, Pat Duffy, Alan K. Kelly, Patrick Lonergan Source Type: research
Free gossypol supplementation frequency and reproductive toxicity in young bulls
The aim of the present study was to analyze seminal quality of young bulls subjected to different frequencies of gossypol supplementation. Forty-eight Nellore bulls, with 19 months of age and weighing 357.8 ± 7.2 kg, were used in this study. Animals were fed with 10.5 kg of standard supplement containing free-gossypol from whole cottonseed (WCS) at the following frequency: 3x/week (G3x), 5x/week (G5x) or 7x/week (G7x - Control). Additionally, a negative control was provided, and the treated a nimals received only mineral supplement (MM) ad libtum. (Source: Theriogenology)
Source: Theriogenology - January 8, 2018 Category: Genetics & Stem Cells Authors: M.F. Duarte J únior, L.K. Hatamoto-Zervoudakis, J.T. Zervoudakis, R.P. Silva-Marques, T.F. Motheo, M. Nichi, P.P. Tsuneda, R.D. Almeida, B.S.E. Santo Source Type: research
Vitrification of testicular tissue from prepubertal cats in cryotubes using different cryoprotectant associations
Protocols for the cryopreservation of testicular tissue are not yet established. In cats, few studies have been conducted on testicular vitrification using different cryoprotectant associations (CPAs). Thus, the objective of this study was to compare the effect of different CPAs on the vitrification of testicular tissue from prepubertal cats in cryotubes. We used 10 pairs of testicles, with each pair divided into 8 fragments that were distributed into different experimental groups. Two of these fragments were allocated into the control group (CG) and the other six were distributed according to the CPAs to be tested (dimeth...
Source: Theriogenology - January 4, 2018 Category: Genetics & Stem Cells Authors: David Baruc Cruvinel Lima, Ticiana Franco Pereira da Silva, Annice Aquino-Cortez, Johanna Leiva Revilla, L úcia Daniel Machado da Silva Source Type: research
Altered expression of IL-1 β, IL-1RI, IL-1RII, IL-1RA and IL-4 could contribute to anovulation and follicular persistence in cattle
Cystic ovarian disease (COD) is an important cause of infertility in dairy cattle. The main signs of this infertility are ovulation failure and follicular persistence. The aim of this study was to examine the expression of the cytokines IL-1 β, IL-1RI, IL-1RII, IL-1RA and IL-4 in ovarian follicular structures at different times of persistence in a model of follicular persistence induced by prolonged administration of progesterone in dairy cows. Protein expression of IL-1β, IL-1RI, IL-1RII, IL-1RA and IL-4 was evaluated by immunohistoc hemistry. (Source: Theriogenology)
Source: Theriogenology - January 3, 2018 Category: Genetics & Stem Cells Authors: A.F. Stassi, M.E. Baravalle, E.M. Belotti, A.N. Amweg, E. Angeli, M.M.L. Vel ázquez, F. Rey, N.R. Salvetti, H.H. Ortega Source Type: research
