Vitrification of testicular tissue from prepubertal cats in cryotubes using different cryoprotectant associations

Protocols for the cryopreservation of testicular tissue are not yet established. In cats, few studies have been conducted on testicular vitrification using different cryoprotectant associations (CPAs). Thus, the objective of this study was to compare the effect of different CPAs on the vitrification of testicular tissue from prepubertal cats in cryotubes. We used 10 pairs of testicles, with each pair divided into 8 fragments that were distributed into different experimental groups. Two of these fragments were allocated into the control group (CG) and the other six were distributed according to the CPAs to be tested (dimethyl sulfoxide (DMSO)/glycerol (GLY), ethylene glycol (EG)/GLY, or DMSO/EG).

Source: Theriogenology - January 4, 2018 Category: Genetics & Stem Cells Authors: David Baruc Cruvinel Lima, Ticiana Franco Pereira da Silva, Annice Aquino-Cortez, Johanna Leiva Revilla, L úcia Daniel Machado da Silva Source Type: research