Exogenous ascorbic acid enhances vitrification survival of porcine in vitro-developed blastocysts but fails to improve the in vitro embryo production outcomes
In this study, the effects of addition of the antioxidant ascorbic acid (AsA) were evaluated during porcine in vitro embryo production (IVP) and vitrification. In experiment 1, the effects of AsA supplementation during IVM, IVF and IVC were evaluated, using a total of 2744 oocytes in six replicates. The IVM, IVF and embryo IVC media were supplemented or not (control) with 50 μg/mL AsA in all possible combinations. No significant effects of AsA were detected in any of the maturation, fertilization or embryo development parameters assessed. (Source: Theriogenology)
Source: Theriogenology - February 17, 2018 Category: Genetics & Stem Cells Authors: A. Nohalez, C.A. Martinez, I. Parrilla, J. Roca, M.A. Gil, H. Rodriguez-Martinez, E.A. Martinez, C. Cuello Source Type: research
Effects of short-term feeding of different sources of fatty acids in pre-mating diets on reproductive performance and blood metabolites of fat-tailed Iranian Afshari ewes
The effects of dietary omega-3 and omega-6 fatty acids (FAs) in pre-mating diets on reproductive performances and metabolic status of ewes have not been well investigated. Therefore, this study investigated the effect of supplementing different source of FAs on different aspects of reproductive performance of fat-tailed Iranian Afshari ewes. Thirty-two cycling, multiparous Afshari ewes were divided into four groups and fed one of the isocaloric and isonitrogenous diets supplemented with Sunflower oil (SFO), Fish oil (FO), calcium salt of palm oil (CaPO) and/or an equal mixture of SFO + FO (SFOFO). (Source: Theriogenology)
Source: Theriogenology - February 15, 2018 Category: Genetics & Stem Cells Authors: H. Mirzaei-Alamouti, Z. Mohammadi, M.H. Shahir, M. Vazirigohar, M. Mansouryar Source Type: research
Brief hypoxic cycles improve uterine contractile function after prolonged hypoxia in term-pregnant but not in nonpregnant rats in vitro
During labour, the uterus itself is vulnerable to hypoxia/ischemia that can occur with each strong contraction and this may ultimately cause dysfunctional labour in some women. Periods of Intermittent re-oxygenations are beneficial to tissues subjected to hypoxia to wash out metabolic by-products that have been accumulated during hypoxic stresses which may affect the tissue viability. We proposed that short intermittent hypoxic episodes may protect the uterus from subsequent sustained long hypoxia. (Source: Theriogenology)
Source: Theriogenology - February 12, 2018 Category: Genetics & Stem Cells Authors: Mohammed Alotaibi Source Type: research
Heat stress responses in spermatozoa: Mechanisms and consequences for cattle fertility
Currently, the world is facing the negative impact of global warming on all living beings. Adverse effects of global warming are also becoming obvious in dairy cattle breeding. In dairy bulls, low fertility has frequently been reported during summer season especially in tropical or subtropical conditions. Typically, spermatozoa at post-meiotic stages of development are more susceptible to heat stress. During this period extensive incorporation of histone modifications and hyperacetylation turns the chromatin into an unstable conformation. (Source: Theriogenology)
Source: Theriogenology - February 12, 2018 Category: Genetics & Stem Cells Authors: Mohammad Bozlur Rahman, Karl Schellander, N úria Llamas Luceño, Ann Van Soom Source Type: research
SNP rs403212791 in exon 2 of the MTNR1A gene is associated with reproductive seasonality in the Rasa aragonesa sheep breed
The aim of this study was to characterize and identify causative SNPs in the MTNR1A gene responsible for the reproductive seasonality traits in the Rasa aragonesa sheep breed. A total of 290 ewes (155, 84 and 51 mature, young and ewe lambs, respectively) from one flock were controlled from January to August. The following three reproductive seasonality traits were considered: the total days of anoestrus (TDA) and the progesterone cycling months (P4CM); both ovarian function seasonality traits based on blood progesterone levels; and the oestrus cycling months (OCM) based on oestrous detection, which indicate behavioural sig...
Source: Theriogenology - February 12, 2018 Category: Genetics & Stem Cells Authors: J.H. Calvo, M. Serrano, A. Martinez-Royo, B. Lahoz, P. Sarto, A. Iba ñez-Deler, J. Folch, J.L. Alabart Source Type: research
Brief hypoxic cycles improve uterine contractile function after prolonged hypoxia in term-pregnant but not in nonpregnant rats in vitro
During labour, the uterus itself is vulnerable to hypoxia/ischemia that can occur with each strong contraction and this may ultimately cause dysfunctional labour in some women. Periods of Intermittent re-oxygenations are beneficial to tissues subjected to hypoxia to wash out metabolic by-products that have been accumulated during hypoxic stresses which may affect the tissue viability. We proposed that short intermittent hypoxic episodes may protect the uterus from subsequent sustained long hypoxia. (Source: Theriogenology)
Source: Theriogenology - February 12, 2018 Category: Genetics & Stem Cells Authors: Mohammed Alotaibi Source Type: research
Heat stress responses in spermatozoa: Mechanisms and consequences for cattle fertility
Currently, the world is facing the negative impact of global warming on all living beings. Adverse effects of global warming are also becoming obvious in dairy cattle breeding. In dairy bulls, low fertility has frequently been reported during summer season especially in tropical or subtropical conditions. Typically, spermatozoa at post-meiotic stages of development are more susceptible to heat stress. During this period extensive incorporation of histone modifications and hyperacetylation turns the chromatin into an unstable conformation. (Source: Theriogenology)
Source: Theriogenology - February 12, 2018 Category: Genetics & Stem Cells Authors: Mohammad Bozlur Rahman, Karl Schellander, N úria Llamas Luceño, Ann Van Soom Source Type: research
SNP rs403212791 in exon 2 of the MTNR1A gene is associated with reproductive seasonality in the Rasa aragonesa sheep breed
The aim of this study was to characterize and identify causative SNPs in the MTNR1A gene responsible for the reproductive seasonality traits in the Rasa aragonesa sheep breed. A total of 290 ewes (155, 84 and 51 mature, young and ewe lambs, respectively) from one flock were controlled from January to August. The following three reproductive seasonality traits were considered: the total days of anoestrus (TDA) and the progesterone cycling months (P4CM); both ovarian function seasonality traits based on blood progesterone levels; and the oestrus cycling months (OCM) based on oestrous detection, which indicate behavioural sig...
Source: Theriogenology - February 12, 2018 Category: Genetics & Stem Cells Authors: J.H. Calvo, M. Serrano, A. Martinez-Royo, B. Lahoz, P. Sarto, A. Iba ñez-Deler, J. Folch, J.L. Alabart Source Type: research
Is the biparietal diameter of fetuses in late gestation too variable to predict readiness for cesarean section in dogs?
The objectives of this study were to determine whether fetal biparietal diameter in late gestation in the dog could be used to predict readiness for cesarean section by having reached a minimum cut-off value and to correlate the biparietal diameter to birth weight. (Source: Theriogenology)
Source: Theriogenology - February 10, 2018 Category: Genetics & Stem Cells Authors: K.G.M. De Cramer, J.O. N öthling Source Type: research
Assessment of the temperature cut-off point by a commercial intravaginal device to predict parturition in Piedmontese beef cows
Dystocic parturitions have an adverse impact on animal productivity and therefore the profitability of the farm. In this regard, accurate prediction of calving is essential since it allows for efficient and prompt assistance of the dam and the calf. Numerous approaches to predict parturition have been studied, among these, measurement of intravaginal temperature (IVT) is the most effective method at the field level. Thus, objectives of this experiment were, 1) to find an IVT cut-off to predict calving within 24 h, and 2) to clarify the use of IVT as an automated method of calving detection in housed beef cows. (Source: Theriogenology)
Source: Theriogenology - February 10, 2018 Category: Genetics & Stem Cells Authors: A. Ricci, V. Racioppi, B. Iotti, A. Bertero, K.F. Reed, O.B. Pascottini, L. Vincenti Source Type: research
Is the biparietal diameter of fetuses in late gestation too variable to predict readiness for cesarean section in dogs?
The objectives of this study were to determine whether fetal biparietal diameter in late gestation in the dog could be used to predict readiness for cesarean section by having reached a minimum cut-off value and to correlate the biparietal diameter to birth weight. (Source: Theriogenology)
Source: Theriogenology - February 10, 2018 Category: Genetics & Stem Cells Authors: K.G.M. De Cramer, J.O. N öthling Source Type: research
Assessment of the temperature cut-off point by a commercial intravaginal device to predict parturition in Piedmontese beef cows
Dystocic parturitions have an adverse impact on animal productivity and therefore the profitability of the farm. In this regard, accurate prediction of calving is essential since it allows for efficient and prompt assistance of the dam and the calf. Numerous approaches to predict parturition have been studied, among these, measurement of intravaginal temperature (IVT) is the most effective method at the field level. Thus, objectives of this experiment were, 1) to find an IVT cut-off to predict calving within 24 h, and 2) to clarify the use of IVT as an automated method of calving detection in housed beef cows. (Source: Theriogenology)
Source: Theriogenology - February 10, 2018 Category: Genetics & Stem Cells Authors: A. Ricci, V. Racioppi, B. Iotti, A. Bertero, K.F. Reed, O.B. Pascottini, L. Vincenti Source Type: research
Enhanced contraception of canine zona pellucida 3 DNA vaccine via targeting DEC-205 in mice
In this study, we designed a canine ZP3 (CZP3) DNA vaccine through targeting DEC-205 (named as pcD-scFv-CZP3c) and investigated its contraceptive effect in mice. Female BALB/c mice were intramuscularly immunized 3 times at 2 weeks intervals. After immunization, humoral and cellular immune responses were detected by ELISA and flow cytometry. The results showed that pcD-CZP3 and pcD-scFv-CZP3c induced CZP3-specific antibody (Ab) responses both in serum and vaginal secretions compared to pcDNA3.1. (Source: Theriogenology)
Source: Theriogenology - February 9, 2018 Category: Genetics & Stem Cells Authors: Ying Wang, Beibei Zhang, Jinyao Li, Adila Aipire, Yijie Li, Fuchun Zhang Source Type: research
Effect of sodium alginate on mouse ovary vitrification
The survival rate of vitrified-thawed ovarian tissues after autotransplantation still needs to be improved. Therefore finding an ideal cryoprectant to reduce the damage to ovaries that caused by vitrification will pave the way for application of ovary cryopreservation on clinics. Experiments were conducted to investigate the effect of sodium alginate in cryoprotectant solution on mouse ovaries during the vitrification process. The ovaries obtained from 6-weeks old CD1 were assigned into six groups from A to F. (Source: Theriogenology)
Source: Theriogenology - February 9, 2018 Category: Genetics & Stem Cells Authors: Gang Liu, Shubin Li, Haihan Yuan, Mengrui Hao, Wurihan, Zhizhong Yun, Jie Zhao, Yuzhen Ma, Yanfeng Dai Source Type: research
