Effects of maternal nutrient restriction followed by realimentation during early and mid-gestation in beef cows. II. Placental development, umbilical blood flow, and uterine blood flow responses to diet alterations
The objectives were to examine the effects of maternal nutrient restriction followed by realimentation during early to mid-gestation on placental development and uterine and umbilical hemodynamics in the beef cow. On day 30 of pregnancy, multiparous, non-lactating beef cows (620.5 ± 11.3 kg) were assigned to 1 of 2 dietary treatments: control (C; 100% National Research Council [NRC] recommendations; n = 18) and restricted (R; 60% NRC; n = 30). On day 85, cows were slaughtered (C, n = 6; R, n = 6), remained on control (CC; n = 12) and restricted (RR ; n = 12), or were realimented to control (...
Source: Theriogenology - April 23, 2018 Category: Genetics & Stem Cells Authors: L.E. Camacho, C.O. Lemley, S.T. Dorsam, K.C. Swanson, K.A. Vonnahme Source Type: research
Laminarin improves developmental competence of porcine early stage embryos by inhibiting oxidative stress
Laminarin (LMA), a β-glucan mixture with good biocompatibility, improves the growth performance and immune response when used as food additives and nutraceuticals. The aim of the present research was to explore the effects of LMA on porcine early stage embryo development, as well as the underlying mechanisms. The res ults showed that the developmental competence of porcine early stage embryos was dramatically improved after LMA supplementation during the in vitro culture period. The presence of 20 μg/mL LMA during the in vitro culture period significantly improved cleavage rate, blastocyst formation rates, ha tching ra...
Source: Theriogenology - April 23, 2018 Category: Genetics & Stem Cells Authors: Hao Jiang, Shuang Liang, Xue-Rui Yao, Yong-Xun Jin, Xing-Hui Shen, Bao Yuan, Jia-Bao Zhang, Nam-Hyung Kim Source Type: research
Effects of maternal nutrient restriction followed by realimentation during early and mid-gestation in beef cows. II. Placental development, umbilical blood flow, and uterine blood flow responses to diet alterations
The objectives were to examine the effects of maternal nutrient restriction followed by realimentation during early to mid-gestation on placental development and uterine and umbilical hemodynamics in the beef cow. On day 30 of pregnancy, multiparous, non-lactating beef cows (620.5 ± 11.3 kg) were assigned to 1 of 2 dietary treatments: control (C; 100% National Research Council [NRC] recommendations; n = 18) and restricted (R; 60% NRC; n = 30). On day 85, cows were slaughtered (C, n = 6; R, n = 6), remained on control (CC; n = 12) and restricted (RR ; n = 12), or were realimented to control (...
Source: Theriogenology - April 23, 2018 Category: Genetics & Stem Cells Authors: L.E. Camacho, C.O. Lemley, S.T. Dorsam, K.C. Swanson, K.A. Vonnahme Source Type: research
Effects of melatonin administration on seminal plasma metabolites and sperm fertilization competence during the non-reproductive season in ram
The purpose of this study was to investigate the effects of ram melatonin treatment on the sperm quality and metabolite composition of the seminal plasma in the non-breeding season. Four mature rams were treated with 54 mg melatonin in March subcutaneous implants and four untreated rams served as control. At 0, 30, 90 and 120 days semen samples were collected and sperm, separated from seminal plasma, was evaluated for its capacity to fertilize and produce embryos in vitro. Seminal plasma metabolites were extract ed and analyzed by capillary electrophoresis/mass spectroscopy. (Source: Theriogenology)
Source: Theriogenology - April 23, 2018 Category: Genetics & Stem Cells Authors: Satta Valentina, Manca Maria Elena, Torres-Rovira Laura, Succu Sara, Mereu Paolo, Nehme Michella, Epifani Gianpaolo, Gallus Marilia, Berlinguer Fiammetta, Naitana Salvatore, Leoni Giovanni Giuseppe Source Type: research
Laminarin improves developmental competence of porcine early stage embryos by inhibiting oxidative stress
Laminarin (LMA), a β-glucan mixture with good biocompatibility, improves the growth performance and immune response when used as food additives and nutraceuticals. The aim of the present research was to explore the effects of LMA on porcine early stage embryo development, as well as the underlying mechanisms. The res ults showed that the developmental competence of porcine early stage embryos was dramatically improved after LMA supplementation during the in vitro culture period. The presence of 20 μg/mL LMA during the in vitro culture period significantly improved cleavage rate, blastocyst formation rates, ha tching ra...
Source: Theriogenology - April 23, 2018 Category: Genetics & Stem Cells Authors: Hao Jiang, Shuang Liang, Xue-Rui Yao, Yong-Xun Jin, Xing-Hui Shen, Bao Yuan, Jia-Bao Zhang, Nam-Hyung Kim Source Type: research
Acute phase proteins and antioxidant responses in queens with pyometra
This study aimed to evaluate the APP and the antioxidant responses at diagnosis and in the post-operative period in feline pyometra. Serum concentrations of serum amyloid A (SAA), haptoglobin (Hp), albumin, total serum thiols (Thiol) and total antioxidant capacity determined by different assays, including trolox equivalent antioxidant capacity (TEAC) assessed by two different methodologies (TEAC1 and TEAC2), ferric reducing ability of plasma (FRAP), and cupric reducing antioxidant capacity (CUPRAC), were determined in 23 queens with pyometra at diagnosis and in 13 healthy control queens submitted to elective ovariohysterec...
Source: Theriogenology - April 21, 2018 Category: Genetics & Stem Cells Authors: Hugo Vilhena, Marta Figueiredo, Jos é Cerón, Josep Pastor, Sónia Miranda, Hélder Craveiro, Maria A. Pires, Fernando Tecles, Camila P. Rubio, Roman Dabrowski, Sofia Duarte, Ana C. Silvestre-Ferreira, Asta Tvarijonaviciute Source Type: research
Effect of hCG and Ovaprim ™ on reproductive characteristics of male Levantine scraper, Capoeta damascina (Valenciennes, 1842)
Species richness and abundance within the genus Capoeta has been depleted. As such, there is great need for developing assisted reproductive technologies for controlling reproduction in captivity. Here, we conducted in vivo studies with single administrations of human chorionic gonadotropin (hCG) and Ovaprim™ [(D-Arg6, Pro9NEt)-sGnRH + domperidone] in wild-caught Levantine scraper, Capoeta damascina and then evaluated milt characteristics, fertilization success, serum sex steroids, and spermatogenesis via hi stological testicular development. (Source: Theriogenology)
Source: Theriogenology - April 19, 2018 Category: Genetics & Stem Cells Authors: Vahid Zadmajid, Somayeh Bashiri, Nastaran Sharafi, Ian Anthony Ernest Butts Source Type: research
Effect of hCG and Ovaprim ™ on reproductive characteristics of male Levantine scraper (Capoeta damascina)
Species richness and abundance within the genus Capoeta has been severely depleted. As such, there is great need for developing assisted reproductive technologies for controlling reproduction in captivity. Here, we conducted in vivo studies with single administrations of human chorionic gonadotropin (hCG) and Ovaprim ™ [(D-Arg6, Pro9NEt)-sGnRH + domperidone] in wild-caught Levantine scraper, Capoeta damascina and then evaluated milt characteristics, fertilization success, serum sex steroids, and spermatogenesis via histological testicular development. (Source: Theriogenology)
Source: Theriogenology - April 19, 2018 Category: Genetics & Stem Cells Authors: Vahid Zadmajid, Somayeh Bashiri, Nastaran Sharafi, Ian Anthony Ernest Butts Source Type: research
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Source: Theriogenology - April 18, 2018 Category: Genetics & Stem Cells Source Type: research
Hypoxia-dependent accumulation of hypoxia-inducible factor-1 alpha induces transient cell cycle arrest in porcine trophectoderm cells
In this study, we determined mechanisms underlying the adaptation of pig embryos to oxygen deficiency in the maternal-conceptus microenvironment in in vitro experiments using our established porcine trophectoderm (pTr) cells in culture. The transition from G1 to S phase in pTr cells was reduced in response to 2% oxygen during a short period ( (Source: Theriogenology)
Source: Theriogenology - April 14, 2018 Category: Genetics & Stem Cells Authors: Wooyoung Jeong, Seoungo Jung, Fuller W. Bazer, Jinyoung Kim Source Type: research
Hypoxia-Dependent Accumulation of Hypoxia-Inducible Factor-1 Alpha Induces Transient Cell Cycle Arrest in Porcine Trophectoderm Cells
In this study, we determined mechanisms underlying the adaptation of pig embryos to oxygen deficiency in the maternal-conceptus microenvironment in in vitro experiments using our established porcine trophectoderm (pTr) cells in culture. The transition from G1 to S phase in pTr cells was reduced in response to 2% oxygen during a short period ( (Source: Theriogenology)
Source: Theriogenology - April 14, 2018 Category: Genetics & Stem Cells Authors: Wooyoung Jeong, Seoungo Jung, Fuller W. Bazer, Jinyoung Kim Source Type: research
Simultaneous effects of IGF1 and Fadrozole on parthenogenesis and pluripotency markers in chicken embryo
So far, a synergistic effect was detected between insulin-like growth factor-I (IGF1) and anti-aromatase for sex reversal and pre-/post-natal growth of fertilized chicken embryo. Here, we hypothesized whether the growth and sexual female-to-male reversal effects of IGF1 and an anti-aromatase, Fadrozole, could improve the development of unfertilized, parthenogenetic chicken embryos. Simultaneous administration of IGF1 and Fadrozole increased the percentage of grade A embryos from 1.7% (no injection group) to 70.6%. (Source: Theriogenology)
Source: Theriogenology - April 13, 2018 Category: Genetics & Stem Cells Authors: Zohreh Hajihoseini, Shahin Eghbalsaied Source Type: research
