Bovine uterus-derived exosomes improve developmental competence of somatic cell nuclear transfer embryos

Exosomes widely exist in various tissues and body fluids, including blood, tissue fluid, and urine. In the present study, exosomes were first isolated from the early luteal phase uterus and confirmed through morphological examination, immunofluorescence (IF) staining of special membrane antigen, and Western blot. The effects of exosomes on the developmental competence of somatic cell nuclear transfer (SCNT) embryos were investigated. Transmission electron microscopy results showed that the isolated exsomes were spherical particles with a 50  nm–150 nm diameter.

Source: Theriogenology - March 22, 2018 Category: Genetics & Stem Cells Authors: Fang Qiao, Hui Ge, Xiaonan Ma, Ying Zhang, Zhenzi Zuo, Mengyun Wang, Yong Zhang, Yongsheng Wang Source Type: research