Molecular characterization of voltage-gated potassium channel (Kv) and its importance in functional dynamics in bull spermatozoa

Present study was undertaken to characterize the voltage gated potassium channel (Kv 1.1) in bull spermatozoa using sixty four ejaculates collected from four Hariana bulls. Functional characterization was undertaken using a selective blocker of Kv channel, 4-Aminopyridine (4-AP) while molecular presence of Kv on bull spermatozoa by immunoblotting and indirect immunofluorescence. Three sets of 100  μL diluted sperm samples namely-negative control (100 μL of sperm dilution medium (SDM) containing 10 × 106 cells), vehicle control (99 μL of SDM containing 10 × 106 cells, and DMSO- 1  μL) and 4-AP treatment group (99 μL of SDM containing 10 × 106 cells, and d rug 1 μL 4-AP) were used in the study.

Source: Theriogenology - March 26, 2018 Category: Genetics & Stem Cells Authors: Rishi Kumar Gupta, Dilip Kumar Swain, Vijay Singh, Mukul Anand, Soumen Choudhury, Sarvajeet Yadav, Atul Saxena, Satish Kumar Garg Source Type: research