Effect of urine contamination on stallion semen freezing ability

Urospermia is a common ejaculatory dysfunction of stallions. Current practice suggests that urine contaminated semen should not be used for cryopreservation. The aim of this study was to determine effects of urine contamination on semen cryopreservation. Sixty-five ejaculates from eight stallions were divided into no urine (CONT), low (20% urine, LOW), and high (50% urine, HIGH) samples. Semen was extended with a commercial cooling extender, cushion-centrifuged, resuspended to 200 million/mL in a commercial egg-yolk based extender, and cryopreserved.

Source: Theriogenology - May 17, 2018 Category: Genetics & Stem Cells Authors: R.E. Ellerbrock, J. Honorato, B.R. Curcio, J.L. Stewart, J.A.T. Souza, C.C. Love, F.S. Lima, I.F. Canisso Source Type: research