Protection of GNRH analogue by chitosan-dextran sulfate nanoparticles for intravaginal application in rabbit artificial insemination

The present study was designed to prove new rabbit insemination extenders containing aminopeptidase inhibitors (AMIs) with or without chitosan (CS)-dextran sulfate (DS) nanoparticles entrapping the GnRH analogue. In addition, different hormone concentrations were tested in these extenders, evaluating their in vivo effect on rabbit reproductive performance after artificial insemination. A total of 911 females were inseminated with semen diluted with the four experimental extenders (C4 group: 4 µg buserelin/doe in control medium (Tris-citric acid-glucose supplemented with bestatin 10 µM and EDTA 20 mM), C5 group: 5 µg of buserelin/doe in control medium, Q4 group: 4 µg of buserelin/doe into CS-DS nanoparticles in control medium, Q5 group: 5 µg of busereline/doe into CS-DS nanoparticles in control medium).
Source: Theriogenology - Category: Genetics & Stem Cells Authors: Source Type: research