Protective effect of Chitosan nanoparticles against the inhibitory effect of linoleic acid supplementation on maturation and developmental competence of bovine oocytes

The current study was conducted to investigate effect of linoleic acid –treated oocytes during in vitro maturation (IVM) on cumulus cell expansion, oocyte nuclear maturation, blastocyst development and DNA damage of cumulus cells and how its effects were alleviated by supplementation of the maturation medium with Chitosan nanoparticles (CSNPs). Inhibitory effects of LA at a concentration (100 μM/ml) or more significantly decreased the percentage of oocytes reaching metaphase II stage, the rate of fully expanded cumulus cells 24 h after IVM and the percentage of blastocyst rate compared with the control (p 
Source: Theriogenology - Category: Genetics & Stem Cells Authors: Source Type: research