Intra cytoplasmic sperm injection (ICSI) of in vitro matured oocytes with stored epididymal spermatozoa in camel (Camelus dromedarius): Effect of exogenous activation on in vitro embryo development

Experiments were conducted to investigate the development of in vitro matured camel oocytes after their intra-cytoplasmic sperm injection (ICSI) with epididymal sperm collected from slaughtered male camels. Ovaries and testes were collected from a local slaughterhouse in normal saline solution (NSS) at 37  °C and on ice (0–1 °C), respectively. Cumulus-oocyte complexes (COCs) aspirated from the follicles were randomly distributed to 4-well culture plates (20–25 COCs/well) containing 500 μL of maturation medium and cultured at 38.5 °C in an atmosphere of 5% CO2 in air for about 30 h.
Source: Theriogenology - Category: Genetics & Stem Cells Authors: Source Type: research