Enhanced contraception of canine zona pellucida 3 DNA vaccine via targeting DEC-205 in mice

In this study, we designed a canine ZP3 (CZP3) DNA vaccine through targeting DEC-205 (named as pcD-scFv-CZP3c) and investigated its contraceptive effect in mice. Female BALB/c mice were intramuscularly immunized 3 times at 2 weeks intervals. After immunization, humoral and cellular immune responses were detected by ELISA and flow cytometry. The results showed that pcD-CZP3 and pcD-scFv-CZP3c induced CZP3-specific Ab responses both in serum and vaginal secretions compared to pcDNA3.1.
Source: Theriogenology - Category: Genetics & Stem Cells Authors: Source Type: research