Protection of bone marrow, mononuclear, and CD34+ cells by enclosing within the biochemical compound solution during and after transplantation
We have chosen collagen, chitosan acetate, hyaluronic acid, and propolis as model biochemical compound solution to determine the influence of cell carrier mechanics on cell viability and functionality during and after transplantation. Suspending of bone marrow (BM), mononuclear (MN), and CD34+ cells into a biochemical compounds solution is an attractive tool to achieve to protect and ensure reproducible deliver. Hyperglycemic rats were randomly divided into 2 groups: to receive no cell treatment or approximately 1 × 105 of BM, MN, and CD34+ cells within the PBS or biochemical compound solution. These cells were infused ...
Source: Cell Biochemistry and Function - August 28, 2017 Category: Biochemistry Authors: Durdi Qujeq, Zeinab Abedian Tags: RESEARCH ARTICLE Source Type: research
Effect of orthodontic force on the expression of PI3K, Akt, and P70S6 K in the human periodontal ligament during orthodontic loading
In this study, we investigated the role of PI3K, Akt, and P70S6 K in human periodontal tissue remodelling during orthodontic loading. The prepared tissue specimens taken from 4 extracted premolars were processed for immunolabelling. The changes in the expression of PI3K, Akt, and P70S6 K in the periodontal tissues were detected by real‐time quantitative‐polymerase chain reaction and Western blot analysis. The results from real‐time quantitative‐polymerase chain reaction and Western blot both showed that the expression of PI3K, Akt, and P70S6 K in the experimental group began to increase at 3 days and increased sig...
Source: Cell Biochemistry and Function - August 28, 2017 Category: Biochemistry Authors: Yunhe Xu, Jiayuan Shen, Fenik Kaml Muhammed, Bowen Zheng, Yuejiao Zhang, Yi Liu Tags: RESEARCH ARTICLE Source Type: research
Serine peptidase inhibitor Kazal type I (SPINK1) promotes BRL ‐3A cell proliferation via p38, ERK, and JNK pathways
In this study, we investigated the role of SPINK1 in a rat liver hepatocyte line of BRL‐3A in vitro. The results showed the upregulation of endogenous Spink1 (gene addition) significantly increased not only the cell viability, cell numbers in S and G2/M phase, but also upregulated the genes/proteins expression related to cell proliferation and anti‐apoptosis in BRL‐3A. In contrast, the cell number in G1 phase and the expression of pro‐apoptosis‐related genes/proteins were significantly decreased. The similar results were observed when the cells were treated with exogenous rat recombinant SPINK1. Immunoblotting su...
Source: Cell Biochemistry and Function - August 27, 2017 Category: Biochemistry Authors: Cuifang Chang, Weiming Zhao, Yaru Luo, Lingling Xi, Shasha Chen, Congcong Zhao, Gaiping Wang, Jianlin Guo, Cunshuan Xu Tags: RESEARCH ARTICLE Source Type: research
RGS3 inhibits TGF ‐β1/Smad signalling in adventitial fibroblasts
In conclusion, these results suggest that RGS3 may provide protection against pathological changes of AFs and the development of atherosclerosis by inhibiting TGF‐β1/Smad signalling. RGS3 may be a potential therapeutic target for atherosclerosis. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - August 27, 2017 Category: Biochemistry Authors: Fang Xu, Ying Liu, Lei Shi, Hongjing Cai, Wei Liu, Yejia Hu, Yuling Li, Wendan Yuan Tags: RESEARCH ARTICLE Source Type: research
Extracellular NAMPT/visfatin causes p53 deacetylation via NAD production and SIRT1 activation in breast cancer cells
In conclusion, the results show that extracellular visfatin produces NAD that causes upregulation of SIRT1 activity and p53 deacetylation. These findings explain the relationship between visfatin and breast cancer progression. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - August 27, 2017 Category: Biochemistry Authors: Kiarash Behrouzfar, Mohammad Alaee, Mitra Nourbakhsh, Zafar Gholinejad, Abolfazl Golestani Tags: RESEARCH ARTICLE Source Type: research
Fractalkine/CX3CR1 induces apoptosis resistance and proliferation through the activation of the AKT/NF ‐κB cascade in pancreatic cancer cells
In this study, we performed an analysis to determine the effects of fractalkine/CX3CR1 on modulating apoptosis and explored the related mechanisms. The expression of fractalkine/CX3CR1 was detected by immunohistochemistry and western blotting. The levels of AKT/p‐AKT, BCL‐xl, and BCL‐2 were detected by western blotting. Then, the effects of exogenous and endogenous fractalkine on the regulation of tumour apoptosis and proliferation were investigated. The mechanism of fractalkine/CX3CR1 on modulating apoptosis in cancer cells through the activation of AKT/NF‐κB/p65 signals was evaluated. The effect of fractalkine o...
Source: Cell Biochemistry and Function - August 27, 2017 Category: Biochemistry Authors: Hui Wang, Jun Cai, Shaoxia Du, Zhongkui Guo, Beibei Xin, Juan Wang, Wei Wei, Xiaohong Shen Tags: RESEARCH ARTICLE Source Type: research
Reptin regulates insulin ‐stimulated Akt phosphorylation in hepatocellular carcinoma via the regulation of SHP‐1/PTPN6
In conclusion, we have uncovered a new function for Reptin in regulating insulin signalling in HCC cells via the regulation of SHP‐1 expression. We suggest that the regulation of insulin sensitivity by Reptin contributes to its oncogenic action in the liver. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - August 22, 2017 Category: Biochemistry Authors: Anne ‐Aurélie Raymond, Joaquim Javary, Osman Breig, Véronique Neaud, Jean Rosenbaum Tags: RESEARCH ARTICLE Source Type: research
Effect of an acute moderate ‐exercise session on metabolic and inflammatory profile of PPAR‐α knockout mice
Conclusion: PPAR‐α seems to be needed for metabolic glucose homeostasis and anti‐inflammatory effect of acute exercise. Its absence may induce over‐expression of pro‐inflammatory cytokines in LPS stimulus. Moreover, moderate exercise or PPAR‐γ agonist did not reverse this response. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - August 1, 2017 Category: Biochemistry Authors: Loreana S. Silveira, Gustavo D. Pimentel, Camila O. Souza, Luana A. Biondo, Alexandre Ab ílio S. Teixeira, Edson A. Lima, Helena A. P. Batatinha, José C. Rosa Neto, Fábio S. Lira Tags: RESEARCH ARTICLE Source Type: research
Wnt/ β‐catenin signalling pathway mediated aberrant hippocampal neurogenesis in kainic acid‐induced epilepsy
Temporal lobe epilepsy is a chronic disorder of nerve system, mainly characterized by hippocampal sclerosis with massive neuronal loss and severe gliosis. Aberrant neurogenesis has been shown in the epileptogenesis process of temporal lobe epilepsy. However, the molecular mechanisms underlying aberrant neurogenesis remain unclear. The roles of Wnt signalling cascade have been well established in neurogenesis during multiple aspects. Here, we used kainic acid‐induced rat epilepsy model to investigate whether Wnt/β‐catenin signalling pathway is involved in the aberrant neurogenesis in temporal lobe epilepsy. Immunostain...
Source: Cell Biochemistry and Function - August 1, 2017 Category: Biochemistry Authors: Zhengyi Qu, Fang Su, Xueting Qi, Jianbo Sun, Hongcai Wang, Zhenkui Qiao, Hong Zhao, Yulan Zhu Tags: RESEARCH ARTICLE Source Type: research
Ursodeoxycholic acid protects cardiomyocytes against cobalt chloride induced hypoxia by regulating transcriptional mediator of cells stress hypoxia inducible factor 1 α and p53 protein
This study provides an insight of UDCA mechanism in protecting cardiomyocytes against hypoxia. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - August 1, 2017 Category: Biochemistry Authors: Anis Syamimi Mohamed, Noorul Izzati Hanafi, Siti Hamimah Sheikh Abdul Kadir, Julina Md Noor, Narimah Abdul Hamid Hasani, Sharaniza Ab Rahim, Rosfaiizah Siran Tags: RESEARCH ARTICLE Source Type: research
ATF1 and RAS in exosomes are potential clinical diagnostic markers for cervical cancer
Cervical cancer is one of the most common cancers among women worldwide. It is highly lethal yet can be treated when found in early stage. Thus, early detection is of significant important for early diagnosis of cervical cancer. Exosomes have been used as biomarkers in clinical diagnosis. It is unknown that whether blood exosomes associated with cervical cancer can be detected and if these exosomes can accurately represent the developmental stage of cervical cancer. Mouse models were made out of a relapsed cervical cancer patient's tumour sample for original and recurrent cervical cancer, and gene analysis in both tumours ...
Source: Cell Biochemistry and Function - August 1, 2017 Category: Biochemistry Authors: Yanhua Shi, Wei Wang, Baozhi Yang, Hongge Tian Tags: RESEARCH ARTICLE Source Type: research
A polysaccharide from Dictyophora indusiata inhibits the immunosuppressive function of cancer ‐associated fibroblasts
In this study, we found that polysaccharides extracted from Dictyophora indusiata may affect the micro‐environment of tumours and inhibit the growth of the tumours. Our results suggest that polysaccharides may modulate negative immune regulation and enhance antitumour immunity, which is important for clinical therapy. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - August 1, 2017 Category: Biochemistry Authors: Shuyu Han, Chaoxiong Ma, Minghua Hu, Yuanyuan Wang, Fangli Ma, Ning Tao, Zhihai Qin Tags: RESEARCH ARTICLE Source Type: research
Effect of metformin and celecoxib on cytotoxicity and release of GDF ‐15 from human mesenchymal stem cells in high glucose condition
Mesenchymal stem cells (MSCs) have therapeutic potential for treatment of diabetes. However, in vitro behavior of MSCs in high glucose condition as well as presence of glucose lowering agents is not fully understood. Because MSCs have an important role in tissue repair, we examined the effects of metformin and celecoxib on viability of MSCs in different glucose conditions. MSCs, from umbilical cord blood, were cultured in normoglycemic (glucose 5.5 mM), midglycemic (glucose 10 mM), and hyperglycemic (glucose 25 mM) conditions, and the cell viability was evaluated by MTT assay. The cytotoxicity and secretion of GDF‐15 ...
Source: Cell Biochemistry and Function - August 1, 2017 Category: Biochemistry Authors: Elaheh Zafarvahedian, Azam Roohi, Mohammad Reza Sepand, Seyed Nasser Ostad, Mohammad Hossein Ghahremani Tags: RESEARCH ARTICLE Source Type: research
Differential expression of microRNAs in TM3 Leydig cells of mice treated with brain ‐derived neurotrophic factor
This study indicated that miRNAs might be involved in the BDNF‐regulated cellular functions of Leydig cells. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - August 1, 2017 Category: Biochemistry Authors: Shan Gao, Chunjin Li, Ying Xu, Shuxiong Chen, Yun Zhao, Lu Chen, Yanwen Jiang, Zhuo Liu, Rong Fan, Liting Sun, Fengge Wang, Xiaoling Zhu, Jing Zhang, Xu Zhou Tags: RESEARCH ARTICLE Source Type: research
HOXA9 is critical in the proliferation, differentiation, and malignancy of leukaemia cells both in vitro and in vivo
In this study, we elucidated the direct correlation between HoxA9 expression and progression of leukaemia using 2 different types of leukaemia cells HL‐60 and MOLT‐3. The HoxA9 expression level was decreased in leukaemia cells with the treatment of all‐trans retinoic acid or arsenic trioxide (As2O3). Downregulation of HoxA9 could impair the proliferation and promote the leukaemia cell death. HoxA9 silencing also potentiated the differentiation of leukaemia cells, and in vivo studies demonstrated that HoxA9 downregulation could interfere the tumour growth. Interestingly, HoxA9 silencing also led to the alteration in m...
Source: Cell Biochemistry and Function - August 1, 2017 Category: Biochemistry Authors: Shibing Chen, Juan Yu, Xin Lv, Lijuan Zhang Tags: RESEARCH ARTICLE Source Type: research
