Increased 4 ‐hydroxy‐2‐nonenal‐induced proteasome dysfunction is correlated with cardiac damage in streptozotocin‐injected rats with isoproterenol infusion
Increase in 4‐hydroxy‐2‐nonenal (4HNE) due to oxidative stress has been observed in a variety of cardiac diseases such as diabetic cardiomyopathy. 4HNE exerts a damaging effect in the myocardium by interfering with subcellular organelles like mitochondria by forming adducts. Therefore, we hypothesized that increased 4HNE adduct formation in the heart results in proteasome inactivation in isoproterenol (ISO)‐infused type 1 diabetes mellitus (DM) rats. Eight‐week‐old male Sprague Dawley rats were injected with streptozotocin (STZ, 65 mg kg−1). The rats were infused with ISO (5 mg kg−1) for 2 weeks b...
Source: Cell Biochemistry and Function - June 6, 2016 Category: Biochemistry Authors: Mandar Deshpande, Vishal R. Mali, Guodong Pan, Jiang Xu, Xiao ‐Ping Yang, Rajarajan A. Thandavarayan, Suresh Selvaraj Palaniyandi Tags: Research Article Source Type: research
Suppressive effect of microRNA ‐29b on hepatic stellate cell activation and its crosstalk with TGF‐β1/Smad3
The microRNA (miR)‐29 family is closely associated with fibrotic processes by virtue of its low expression in many tissues during organ fibrosis. The present study investigated whether miR‐29b overexpression suppressed hepatic stellate cell (HSC) activation and its interactions with transforming growth factor (TGF)‐β1/mothers against decapentaplegic homolog 3 (Smad3), a classical signal transduction pathway contributing to the activation of HSCs. The results showed that transfection of LX‐2 (human HSC) cells with miR‐29b mimic or pSUPER‐Smad3 silencing (si)RNA resulted in significantly increased expression of ...
Source: Cell Biochemistry and Function - June 6, 2016 Category: Biochemistry Authors: Chunli Liang, Shurui Bu, Xiaoming Fan Tags: Research Article Source Type: research
Platelet aggregation and serum adenosine deaminase (ADA) activity in pregnancy associated with diabetes, hypertension and HIV
In this study, the increased platelet aggregation and ADA activity in pregnancy and pregnancy‐associated diseases suggest that platelet aggregation and ADA activity could serve as peripheral markers for the development of effective therapy in the maintenance of homeostasis and some inflammatory process in these pathophysiological conditions. Copyright © 2016 John Wiley & Sons, Ltd. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - June 6, 2016 Category: Biochemistry Authors: Claudio A. M. Leal, Daniela B. R. Leal, Stephen A. Adefegha, Vera M. Morsch, José E. P. Silva, João F. P. Rezer, Clarissa M. L. Schrekker, Faida H. Abdalla, Maria R. C. Schetinger Tags: Research Article Source Type: research
Suppressive effect of microRNA‐29b on hepatic stellate cell activation and its crosstalk with TGF‐β1/Smad3
The microRNA (miR)‐29 family is closely associated with fibrotic processes by virtue of its low expression in many tissues during organ fibrosis. The present study investigated whether miR‐29b overexpression suppressed hepatic stellate cell (HSC) activation and its interactions with transforming growth factor (TGF)‐β1/mothers against decapentaplegic homolog 3 (Smad3), a classical signal transduction pathway contributing to the activation of HSCs. The results showed that transfection of LX‐2 (human HSC) cells with miR‐29b mimic or pSUPER‐Smad3 silencing (si)RNA resulted in significantly increased expression of ...
Source: Cell Biochemistry and Function - June 6, 2016 Category: Biochemistry Authors: Chunli Liang, Shurui Bu, Xiaoming Fan Tags: Research Article Source Type: research
Hepatoma‐derived growth factor promotes growth and metastasis of hepatocellular carcinoma cells
In conclusion, HDGF was overexpressed in HCC patients and cells, and HDGF might be closely correlated with HCC metastasis via regulating ERK signalling pathway. Copyright © 2016 John Wiley & Sons, Ltd. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - June 6, 2016 Category: Biochemistry Authors: Guang‐yun Yang, Ai‐qun Zhang, Jing Wang, Chong‐hui Li, Xian‐qiang Wang, Ke Pan, Cheng Zhou, Jia‐hong Dong Tags: Research Article Source Type: research
Issue Information
No abstract is available for this article. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - June 6, 2016 Category: Biochemistry Tags: Issue Information Source Type: research
Down‐regulation of semaphorin 3F in rat retinal ganglion cells in response to optic nerve crush
Glaucoma is characterized by degeneration of optic nerve axons and death of retinal ganglion cells (RGCs). Nerve crush and axotomy of the optic nerve are studied as models of RGC death in glaucoma and of axon regeneration. The mechanisms underlying the response of RGCs to axonal injury remain unclear, however. We have now examined the effects of optic nerve crush on the expression of members of the semaphorin family of neuronal guidance proteins in the rat retina. The expression of semaphorin 3F (Sema3F) in the retina was down‐regulated at both the mRNA and protein levels at 7 days after optic nerve injury, whereas tha...
Source: Cell Biochemistry and Function - May 31, 2016 Category: Biochemistry Authors: Ji‐Ae Ko, Akira Minamoto, Yosuke Sugimoto, Yoshiaki Kiuchi Tags: Research Article Source Type: research
Ceranib‐2‐induced suicidal erythrocyte death
Ceramide is known to trigger apoptosis of nucleated cells and eryptosis of erythrocytes. Eryptosis is characterized by cell shrinkage and cell membrane scrambling with phosphatidylserine translocation to the erythrocyte surface. Besides ceramide, stimulators of eryptosis include increase of cytosolic Ca2+‐activity ([Ca2+]i) and oxidative stress. Ceramide is degraded by acid ceramidase and inhibition of the enzyme similarly triggers apoptosis. The present study explored, whether ceramidase inhibitor Ceranib‐2 induces eryptosis. Flow cytometry was employed to quantify phosphatidylserine‐exposure at the cell surface fro...
Source: Cell Biochemistry and Function - May 31, 2016 Category: Biochemistry Authors: Elena Signoretto, Jens Zierle, Abdulla Al Mamun Bhuyan, Michela Castagna, Florian Lang Tags: Research Article Source Type: research
Increased 4‐hydroxy‐2‐nonenal‐induced proteasome dysfunction is correlated with cardiac damage in streptozotocin‐injected rats with isoproterenol infusion
Increase in 4‐hydroxy‐2‐nonenal (4HNE) due to oxidative stress has been observed in a variety of cardiac diseases such as diabetic cardiomyopathy. 4HNE exerts a damaging effect in the myocardium by interfering with subcellular organelles like mitochondria by forming adducts. Therefore, we hypothesized that increased 4HNE adduct formation in the heart results in proteasome inactivation in isoproterenol (ISO)‐infused type 1 diabetes mellitus (DM) rats. Eight‐week‐old male Sprague Dawley rats were injected with streptozotocin (STZ, 65 mg kg−1). The rats were infused with ISO (5 mg kg−1) for 2 weeks b...
Source: Cell Biochemistry and Function - May 31, 2016 Category: Biochemistry Authors: Mandar Deshpande, Vishal R. Mali, Guodong Pan, Jiang Xu, Xiao‐Ping Yang, Rajarajan A. Thandavarayan, Suresh Selvaraj Palaniyandi Tags: Research Article Source Type: research
Accumulation of apoptosis ‐insensitive human bone marrow‐mesenchymal stromal cells after long‐term expansion
In this study, we investigated whether long‐term‐cultured human bone marrow mesenchymal stromal cells (MSCs) are insensitive to apoptotic stimulation. To examine this, we established replicative senescent cells from long‐term cultures of human bone marrow MSCs. Senescent cells were identified based on declining population doublings, increased expression of senescence markers p16 and p53 and increased senescence‐associated β‐gal activity. In cell viability assays, replicative senescent MSCs in late passages (i.e. 15–19 passages) resisted damage induced by oxidative stress more than those in early passages did (...
Source: Cell Biochemistry and Function - May 21, 2016 Category: Biochemistry Authors: Sin ‐Gu Jeong, Goang‐Won Cho Tags: Research Article Source Type: research
Differential expression of long non ‐coding RNAs in hyperoxia‐induced bronchopulmonary dysplasia
Bronchopulmonary dysplasia (BPD) is a common complication of premature birth that seriously affects the survival rate and quality of life among preterm neonates. Long non‐coding RNAs (lncRNAs) have been implicated in many human diseases. However, the role of lncRNAs in the pathogenesis of BPD remains poorly understood. Here, we exposed neonatal C57BL/6J mice to 95% concentrations of ambient oxygen and established a mouse lung injury model that mimicked human BPD. Next, we compared lncRNA and messenger RNA (mRNA) expression profiles between BPD and normal lung tissues using a high‐throughput mouse lncRNA + mRNA arra...
Source: Cell Biochemistry and Function - May 2, 2016 Category: Biochemistry Authors: Tian ‐Ping Bao, Rong Wu, Huai‐Ping Cheng, Xian‐Wei Cui, Zhao‐Fang Tian Tags: Research Article Source Type: research
Issue Information
No abstract is available for this article. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - April 3, 2016 Category: Biochemistry Tags: Issue Information Source Type: research
Accumulation of apoptosis‐insensitive human bone marrow‐mesenchymal stromal cells after long‐term expansion
In this study, we investigated whether long‐term‐cultured human bone marrow mesenchymal stromal cells (MSCs) are insensitive to apoptotic stimulation. To examine this, we established replicative senescent cells from long‐term cultures of human bone marrow MSCs. Senescent cells were identified based on declining population doublings, increased expression of senescence markers p16 and p53 and increased senescence‐associated β‐gal activity. In cell viability assays, replicative senescent MSCs in late passages (i.e. 15–19 passages) resisted damage induced by oxidative stress more than those in early passages did (...
Source: Cell Biochemistry and Function - March 31, 2016 Category: Biochemistry Authors: Sin‐Gu Jeong, Goang‐Won Cho Tags: Research Article Source Type: research
Proteomic profiling of lymphedema development in mouse model
In this study, we investigated differential protein expressions in sham‐operated and lymphedema‐operated mice for 3 days, using two‐dimensional gel electrophoresis (2‐DE) and mass spectrometry analysis. Male improved methodology for culturing noninbred (ICR) mice developed lymphedema in the right hindlimb. Twenty functional proteins were found to be differentially expressed between lymphedema induced‐right leg tissue and normal left leg tissue. Out of these proteins, the protein levels of apolipoprotein A‐1 preprotein, alpha‐actinin‐3, mCG21744, parkinson disease, serum amyloid P‐component precursor, an...
Source: Cell Biochemistry and Function - March 31, 2016 Category: Biochemistry Authors: Joomin Lee, Haeun Song, Kangsan Roh, Sungrae Cho, Sukchan Lee, Chang‐Hwan Yeom, Seyeon Park Tags: Research Article Source Type: research
Differential expression of long non‐coding RNAs in hyperoxia‐induced bronchopulmonary dysplasia
Bronchopulmonary dysplasia (BPD) is a common complication of premature birth that seriously affects the survival rate and quality of life among preterm neonates. Long non‐coding RNAs (lncRNAs) have been implicated in many human diseases. However, the role of lncRNAs in the pathogenesis of BPD remains poorly understood. Here, we exposed neonatal C57BL/6J mice to 95% concentrations of ambient oxygen and established a mouse lung injury model that mimicked human BPD. Next, we compared lncRNA and messenger RNA (mRNA) expression profiles between BPD and normal lung tissues using a high‐throughput mouse lncRNA + mRNA arra...
Source: Cell Biochemistry and Function - March 31, 2016 Category: Biochemistry Authors: Tian‐Ping Bao, Rong Wu, Huai‐Ping Cheng, Xian‐Wei Cui, Zhao‐Fang Tian Tags: Research Article Source Type: research
