Issue Information
No abstract is available for this article. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - December 21, 2015 Category: Biochemistry Tags: Issue Information Source Type: research
Involvement of autophagy in the procedure of endoplasmic reticulum stress introduced apoptosis in bone marrow mesenchymal stem cells from nonobese diabetic mice
In this study, endoplasmic reticulum (ER) stress and autophagy were evidenced in BM‐MSCs from NOD mice for the first time. We found the ER stress‐mediated apoptosis was supported by the up‐regulation of ER stress markers including augmented phosphorylation of phosphorylated protein kinase RNA‐like ER kinase and eukaryotic translation initiator factor 2α as well as cleavage of caspase‐3. Evidence of autophagy included the formation of the acidic vesicular organelles and increase of LC3 accumulation. Intriguingly, blockage of ER stress could reduce the apoptosis of BM‐MSCs from NOD mice and alleviated accumulati...
Source: Cell Biochemistry and Function - December 1, 2015 Category: Biochemistry Authors: Yan Meng, Juan Ji, Wei Tan, Genkai Guo, Yunfei Xia, Chun Cheng, Zhifeng Gu, Zhiwei Wang Tags: Research Article Source Type: research
α‐Lipoic acid inhibits sevoflurane‐induced neuronal apoptosis through PI3K/Akt signalling pathway
This study elucidated the potential clinical role of α‐lipoic acid, providing a promising way in the prevention and treatment of long‐term cognitive impairment induced by sevoflurane general anesthesia. Copyright © 2016 John Wiley & Sons, Ltd. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - December 1, 2015 Category: Biochemistry Authors: Rong Ma, Xiang Wang, Peipei Peng, Jingwei Xiong, Hongquan Dong, Lixia Wang, Zhengnian Ding Tags: Research Article Source Type: research
Cell culture density affects the proliferation activity of human adipose tissue stem cells
In this study, we investigated the effect of cell density on the proliferation activity of human mesenchymal stem cells (MSCs) derived from adipose tissue (AT‐MSCs) over time in culture. Passage #4 (P4) and #12 (P12) AT‐MSCs from two donors were plated at a density of 200 (culture condition 1, CC1) or 5000 (culture condition 2, CC2) cells cm−2. After 7 days of incubation, P4 and P12 AT‐MSCs cultured in CC1 were thin and spindle‐shaped, whereas those cultured in CC2 had extensive cell‐to‐cell contacts and an expanded cell volume. In addition, P4 and P12 AT‐MSCs in CC1 divided more than three times, while...
Source: Cell Biochemistry and Function - December 1, 2015 Category: Biochemistry Authors: Dae Seong Kim, Myoung Woo Lee, Young Jong Ko, Yong Hoon Chun, Hyung Joon Kim, Ki Woong Sung, Hong Hoe Koo, Keon Hee Yoo Tags: Research Article Source Type: research
Stat3‐siRNA inhibits the growth of gastric cancer in vitro and in vivo
Gastric cancer remains one of the most prevalent and lethal malignancies in the world. Despite new advances in treatment and diagnosis, patients with advanced gastric cancer are still difficult to cure resulting in a high mortality rate and poor prognosis. Signal transducer and activator of transcription 3 (Stat3) is observed aberrant in multiple tumours, including gastric cancer. Stat3 overexpression was confirmed performing a vital role in tumorigenesis. In the present study, we constructed a pSi‐Stat3 plasmid to silence Stat3 and investigated the effect of pSi‐Stat3 on cell proliferation, apoptosis and cell cycle pr...
Source: Cell Biochemistry and Function - October 20, 2015 Category: Biochemistry Authors: Ying Sun, Bao‐feng Guo, Li‐bo Xu, Jia‐teng Zhong, Zhe‐wen Liu, Hang Liang, Nai‐yan Wen, Wen‐jing Yun, Ling Zhang, Xue‐jian Zhao Tags: Research Article Source Type: research
Role of Spm–Cer‐S1P signalling pathway in MMP‐2 mediated U46619‐induced proliferation of pulmonary artery smooth muscle cells: protective role of epigallocatechin‐3‐gallate
During remodelling of pulmonary artery, marked proliferation of pulmonary artery smooth muscle cells (PASMCs) occurs, which contributes to pulmonary hypertension. Thromboxane A2 (TxA2) has been shown to produce pulmonary hypertension. The present study investigates the inhibitory effect of epigallocatechin‐3‐gallate (EGCG) on the TxA2 mimetic, U46619‐induced proliferation of PASMCs. U46619 at a concentration of 10 nM induces maximum proliferation of bovine PASMCs. Both pharmacological and genetic inhibitors of p38MAPK, NF‐κB and MMP‐2 significantly inhibit U46619‐induced cell proliferation. EGCG markedly abr...
Source: Cell Biochemistry and Function - October 20, 2015 Category: Biochemistry Authors: Animesh Chowdhury, Jaganmay Sarkar, Tapati Chakraborti, Sajal Chakraborti Tags: Research Article Source Type: research
Issue Information
No abstract is available for this article. (Source: Cell Biochemistry and Function)
Source: Cell Biochemistry and Function - October 2, 2015 Category: Biochemistry Tags: Issue Information Source Type: research
Stress response factors as hub‐regulators of microRNA biogenesis: implication to the diseased heart
MicroRNAs (miRNAs) are important regulators of heart function and then an intriguing therapeutic target for plenty of diseases. The problem raised is that many data in this area are contradictory, thus limiting the use of miRNA‐based therapy. The goal of this review is to describe the hub‐mechanisms regulating the biogenesis and function of miRNAs, which could help in clarifying some contradictions in the miRNA world. With this scope, we analyse an array of factors, including several known agents of stress response, mediators of epigenetic changes, regulators of alternative splicing, RNA editing, protein synthesis and ...
Source: Cell Biochemistry and Function - October 1, 2015 Category: Biochemistry Authors: Veronika Gurianova, Dmytro Stroy, Rachele Ciccocioppo, Iveta Gasparova, Daniel Petrovic, Miroslav Soucek, Victor Dosenko, Peter Kruzliak Tags: Review Article Source Type: research
1,4‐Dihydropyridine derivatives without Ca2+‐antagonist activity up‐regulate Psma6 mRNA expression in kidneys of intact and diabetic rats
Impaired degradation of proteins by the ubiquitin–proteasome system (UPS) is observed in numerous pathologies including diabetes mellitus (DM) and its complications. Dysregulation of proteasomal degradation might be because of altered expression of genes and proteins involved in the UPS. The search for novel compounds able to normalize expression of the UPS appears to be a topical problem. A novel group of 1,4‐dihydropyridine (1,4‐DHP) derivatives lacking Ca2+‐antagonists activities, but capable to produce antidiabetic, antioxidant and DNA repair enhancing effects, were tested for ability to modify Psma6 mRNA expre...
Source: Cell Biochemistry and Function - October 1, 2015 Category: Biochemistry Authors: Kristīne Ošiņa, Evita Rostoka, Jelizaveta Sokolovska, Natalia Paramonova, Egils Bisenieks, Gunars Duburs, Nikolajs Sjakste, Tatjana Sjakste Tags: Rapid Communication Source Type: research
The conditioned medium from osteo‐differentiating human mesenchymal stem cells affects the viability of triple negative MDA‐MB231 breast cancer cells
This study aimed to investigate the effect of conditioned media (CM) from osteo‐differentiating and adipo‐differentiating human mesenchymal stem cells (MSCs) isolated from lipoaspirates of healthy female donors on the viability of triple‐negative breast cancer cells MDA‐MB231. The CM of undifferentiated and differentiating MSCs were collected after 7, 14, 21 and 28 days of culture. The effects of MSC CM on cell proliferation were assessed using an 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay after 24 h. The effects of osteo‐differentiating cell CM on apoptotic promotion...
Source: Cell Biochemistry and Function - October 1, 2015 Category: Biochemistry Authors: Mariangela Librizzi, Edda Tobiasch, Claudio Luparello Tags: Research Article Source Type: research
Platelet endothelial cell adhesion molecule‐1 (PECAM1) plays a critical role in the maintenance of human vascular endothelial barrier function
This study aims to investigate the role of platelet endothelial cell adhesion molecule‐1 (PECAM1) in the maintenance of the vascular endothelial barrier integrate. Human umbilical vein endothelial cells (HUVECs) were cultured into monolayers using as an in vitro model to assess the endothelial barrier function. Knockdown of the gene of PECAM1 markedly reduced the transendothelial resistance and increased the permeability of the HUVEC monolayers. From the wild HUVECs, we detected a complex of PECAM1, claudin1, occluding and endothelial cell selective adhesion molecule (ESAM); such a complex was not detected in the PECAM1...
Source: Cell Biochemistry and Function - October 1, 2015 Category: Biochemistry Authors: Qi Ren, Limin Ren, Changjie Ren, Xuefei Liu, Chun Dong, Xinghua Zhang Tags: Research Article Source Type: research
Effects of physical exercise training in DNA damage and repair activity in humans with different genetic polymorphisms of hOGG1 (Ser326Cys)
The main purpose of this pilot study was to investigate the possible influence of genetic polymorphisms of the hOGG1 (Ser326Cys) gene in DNA damage and repair activity by 8‐oxoguanine DNA glycosylase 1 (OGG1 enzyme) in response to 16 weeks of combined physical exercise training. Thirty‐two healthy Caucasian men (40–74 years old) were enrolled in this study. All the subjects were submitted to a training of 16 weeks of combined physical exercise. The subjects with Ser/Ser genotype were considered as wild‐type group (WTG), and Ser/Cys and Cys/Cys genotype were analysed together as mutant group (MG). We used come...
Source: Cell Biochemistry and Function - October 1, 2015 Category: Biochemistry Authors: Jorge Pinto Soares, Ana Inês Silva, Amélia M. Silva, Vanessa Almeida, Manuela Matos, Isabel Gaivão, Maria Paula Mota Tags: Research Article Source Type: research
miR‐539 induces cell cycle arrest in nasopharyngeal carcinoma by targeting cyclin‐dependent kinase 4
Abstract
Dysregulation of microRNAs has been demonstrated to contribute to malignant progression of cancers, including nasopharyngeal carcinoma (NPC). miR‐539 was previously reported to be significantly downregulated in osteosarcoma. However, the potential role and mechanism of action of miR‐539 in the initiation and progression of NPC remain largely unknown. Quantitative reverse transcription (RT)‐PCR demonstrated that miR‐539 was significantly downregulated in NPC tumour tissues compared with nontumour tissues. The cell viability, colony formation assay and tumourigenicity assays in nude mice showed that miR‐53...
Source: Cell Biochemistry and Function - October 1, 2015 Category: Biochemistry Authors: Ling‐yan Lv, Yu‐zhi Wang, Qian Zhang, Hong‐rui Zang, Xing‐jie Wang Tags: Research Article Source Type: research
Phenethyl isothiocyanate potentiates anti‐tumour effect of doxorubicin through Akt‐dependent pathway
The present study aims to investigate the in vivo and in vitro anti‐tumour properties of phenethyl isothiocyanate (PEITC) alone and in combination with doxorubicin (Dox). The anti‐tumour activity was evaluated in vitro by MTT assay using cultured human breast cancer cell line (MCF‐7) and human hepatoma cell line (HepG‐2) cell lines. In vivo, Ehrlich solid tumour model was used. Tumour volume, weight and antioxidant parameters were determined. Immunohistochemistry analysis for active (cleaved) caspase‐3 was also performed. We tested the effect of PEITC treatment on pAkt/Akt ratio, NF‐κB p65 DNA binding activity...
Source: Cell Biochemistry and Function - October 1, 2015 Category: Biochemistry Authors: Nada H. Eisa, Nehal M. ElSherbiny, Abdelhadi M. Shebl, Laila A. Eissa, Mamdouh M. El‐Shishtawy Tags: Research Article Source Type: research
PUGNAc induces protein ubiquitination in C2C12 myotube cells
O‐linked β‐N‐acetylglucosaminylation (O‐GlcNAcylation) regulates many cellular processes including the cell cycle, cell signaling, and protein trafficking. Dysregulation of O‐GlcNAcylation may be involved in the development of insulin resistance and type 2 diabetes. Therefore, it is necessary to identify cellular proteins that are induced by elevated O‐GlcNAcylation. Here, using adenosine 5′‐triphosphate affinity chromatography, we employed a proteomic approach in order to identify differentially expressed proteins in response to treatment with the O‐GlcNAcase inhibitor, O‐(2‐acetamido‐2‐deoxy‐...
Source: Cell Biochemistry and Function - October 1, 2015 Category: Biochemistry Authors: Ja‐Hye Park, Jeong‐Eun Lee, Pyong‐Gon Moon, Moon‐Chang Baek Tags: Research Article Source Type: research
